Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	746,576	(G)5→6	coding (642/792 nt)	nei →	endonuclease VIII

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	746,571	1	.	G	100.0%	135.2 / NA	39	R213G (CGG→GGG)	nei	endonuclease VIII
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (24/15);  total (24/15)

TTATTTGCGGGTGGAGATCCTCTGGCAGGTTGGGTTGACTGGAAATCATAAAGCGAAAGATCTCAATGCGGCGCAACTGGATGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGC‑GGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTT  >  NC_000913/746441‑746698                                                                                                                                    |                                                                                                                                ttattTGCGGGTGGAGATCCTCTGGCAGGTTGGGTTGACTGGAAATCATAAAGCGAAAGATCTCAATGCGGCGCAACTGGATGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGC‑ggggggcag                                                                                                                        >  1:13033/1‑136 (MQ=255) ttattTGCGGGTGGAGATCCTCTGGCAGGTTGGGTTGACTGGAAATCATAAAGCGAAAGATCTCAATGCGGCGCAACTGGATGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGC‑ggggggcag                                                                                                                        >  2:627272/1‑136 (MQ=255) ttattTGCGGGTGGAGATCCTCTGGCAGGTTGGGTTGACTGGAAATCATAAAGCGAAAGATCTCAATGCGGCGCAACTGGATGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGC‑ggggggcag                                                                                                                        >  2:27632/1‑136 (MQ=255)  tattTGCGGGTGGAGATCCTCTGGCAGGTTGGGTTGACTGGAAATCATAAAGCGAAAGATCTCAATGCGGCGCAACTGGATGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGC‑ggggggcagg                                                                                                                       <  2:404079/140‑6 (MQ=255)       gCGGGTGGAGATCCTCTGGCAGGTTGGGTTGACTGGAAATCATAAAGCGAAAGATCTCAATGCGGCGCAACTGGATGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGAt                                                                                                                   <  1:27632/140‑1 (MQ=255)       gCGGGTGGAGATCCTCTGGCAGGTTGGGTTGACTAGAAATCATAAAGCGAAAGATCTCAATGCGGCGCAACTGGATGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGAt                                                                                                                   <  1:627272/140‑1 (MQ=255)       gCGGGTGGAGAACCTCTGGCCGGTTGGGTTGACTGGAAATCATAAAGCGAAAGATCTCTCTGCGGCGCAACTGGATGCACTCGCACACGCGTTACTGGAGATTCATCGATTTTCCTACGCTACGCGGGGGGCAGGGGGAt                                                                                                                   >  2:255165/1‑140 (MQ=255)           gTGGAGATCCTCTGGCAGGTTGGGTTGACTGGAAATCATAAAGCGAAAGATCTCAATGCGGCGCAACTGGATGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATgaga                                                                                                               >  2:20622/1‑140 (MQ=255)            tGGAGATCCTCTGGCAGGTTGGGTTGACTGGAAATCATAAAGCGAAAGATCTCAATGCGGCGCAACTGGATGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATg                                                                                                                  >  2:49162/1‑136 (MQ=255)            tGGAGATCCTCTGGCAGGTTGGGTTGACTGGAAATCATAAAGCGAAAGATCTCAATGCGGCGCAACTGGATGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATg                                                                                                                  <  1:49162/136‑1 (MQ=255)            tGGAGATCCTCTGGCAGGTTGGGTTGACTGGAAATCATAAAGCGAAAGATCTCAATGCGGCGCAACTGGATGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGC‑ggggggcag                                                                                                                        >  2:104220/1‑125 (MQ=255)            tGGAGATCCTCTGGCAGGTTGGGTTGACTGGAAATCATAAAGCGAAAGATCTCAATGCGGCGCAACTGGATGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGC‑ggggggcag                                                                                                                        <  1:104220/129‑5 (MQ=255)                     tctGGCAGGTTGGGTTGACTGGAAATCATAAAGCGAAAGATCTCAATGCGGCGCAACTGGATGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGcatca                                                                                                     >  2:322364/1‑140 (MQ=255)                          cAGGTTGGGTTGACTGGAAATCATAAAGCGAAAGATCTCAATGCGGCGCAACTGGATGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTGCGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATgggg                                                                                                <  1:323415/140‑1 (MQ=255)                               tgggttgACTGGAAATCATAAAGCGAAAGATCTCAATGCGGCGCAACTGGATGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTg                                                                                           >  2:385071/1‑140 (MQ=255)                                  gttgACTGGAAATCATAAAGCGAAAGATCTCAATGCGGCGCAACTGGATGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGttt                                                                                        <  1:385071/140‑1 (MQ=255)                                       cTGGAAATCATAAAGCGAAAGATCTCAATGCGGCGCAACTGGATGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCtt                                                                                   >  1:341320/1‑140 (MQ=255)                                                      cGAAAGATCTCAATGCGGCGCAACTGGATGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCg                                                                    <  2:537627/140‑1 (MQ=255)                                                      cGAAAGATCTCAATGCGGCGCAACTGGATGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCg                                                                    <  2:13033/140‑1 (MQ=255)                                                         aaGATCTCAATGCGGCGCAACTGGATGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCgaga                                                                 >  1:414121/1‑140 (MQ=255)                                                                cAATGCGGCGCAACTGGATGCCCTCGCACGCGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGaa                                                          <  2:341320/140‑1 (MQ=255)                                                                        cgcAACTGGATGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCg                                                                    <  2:430409/122‑1 (MQ=255)                                                                        cgcAACTGGATGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCg                                                                    >  1:430409/1‑122 (MQ=255)                                                                        cgcAACTGGATGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGa                                                  >  1:370474/1‑140 (MQ=255)                                                                           aaCTGGATGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACg                                               >  2:241728/1‑140 (MQ=255)                                                                                 aTGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTgg                                         <  2:414121/140‑1 (MQ=255)                                                                                    cACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGgcag                                      <  1:254518/140‑1 (MQ=255)                                                                                    cACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGgcag                                      >  2:735569/1‑140 (MQ=255)                                                                                            cacGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTg                              >  1:60757/1‑140 (MQ=255)                                                                                            cacGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTg                              >  2:641197/1‑140 (MQ=255)                                                                                             acGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTga                             <  1:735569/140‑1 (MQ=255)                                                                                               gcgTTACTGGAGATTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTgaga                           >  2:462445/1‑140 (MQ=255)                                                                                                     cTGGAGATTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCa                     <  1:241728/140‑1 (MQ=255)                                                                                                         agaTTCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCt                 >  2:186934/1‑140 (MQ=255)                                                                                                             tCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCAtt                               <  2:810429/122‑1 (MQ=255)                                                                                                             tCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCAtt                               >  1:810429/1‑122 (MQ=255)                                                                                                             tCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCa             >  1:288624/1‑140 (MQ=255)                                                                                                             tCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCa             >  2:219228/1‑140 (MQ=255)                                                                                                             tCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCa             >  2:120304/1‑140 (MQ=255)                                                                                                             tCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCa             >  1:549984/1‑140 (MQ=255)                                                                                                             tCCTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCa             >  1:390151/1‑140 (MQ=255)                                                                                                               cTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCAc                    <  2:403561/131‑1 (MQ=255)                                                                                                               cTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCAc                    >  1:403561/1‑131 (MQ=255)                                                                                                               cTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCAtc           >  2:163383/1‑140 (MQ=255)                                                                                                               cTCGATTTTCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCAtc           >  1:85315/1‑140 (MQ=255)                                                                                                                    ttttCCTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGcc      <  1:186934/140‑1 (MQ=255)                                                                                                                        ccTACGCTACGCGGGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGtt  >  1:668225/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                TTATTTGCGGGTGGAGATCCTCTGGCAGGTTGGGTTGACTGGAAATCATAAAGCGAAAGATCTCAATGCGGCGCAACTGGATGCACTCGCACACGCGTTACTGGAGATTCCTCGATTTTCCTACGCTACGC‑GGGGGCAGGTGGATGAGAATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTT  >  NC_000913/746441‑746698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑