| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,972,741 | (G)6→7 | intergenic (‑50/+95) | tar ← / ← cheW | methyl‑accepting chemotaxis protein Tar/chemotaxis protein CheW |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,972,735 | 1 | . | G | 87.1% | 92.6 / 7.4 | 31 | intergenic (‑44/+101) | tar/cheW | methyl‑accepting chemotaxis protein Tar/chemotaxis protein CheW |
| Reads supporting (aligned to +/- strand): ref base . (2/2); new base G (22/5); total (24/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
AAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑A‑GGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAG > NC_000913/1972598‑1972868 | aagaaAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAa‑gg‑a‑gg > 1:257604/1‑140 (MQ=255) gaaAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑A‑gggg < 2:257604/140‑1 (MQ=255) aaaaCAGACTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑A‑gggg‑ggga < 1:87198/140‑3 (MQ=255) tAACAGTGCGAATACCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACccgcc > 2:30830/1‑140 (MQ=255) tGCGAATACCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGaaa > 1:236284/1‑140 (MQ=255) cGAATACCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAAtt > 1:99754/1‑140 (MQ=255) cGAATACCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAAtt > 1:710502/1‑140 (MQ=255) acagccccAGCACCATTACCAACAGCGTGACTACGGGGATACGGTTAATCAAAAGGCCCCTTCCTAAAAACAAGTTGATCTCTTTATCGGAAAGGG‑G‑GGGG‑GG‑AAA‑CTTTATTGCTGATGCCCCCCGCCGCGAAATTGaa < 2:495444/136‑1 (MQ=255) cACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAAcc < 1:30830/140‑1 (MQ=255) aTTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGAt > 1:124204/1‑140 (MQ=255) ttACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAAcccctgt > 1:495444/1‑136 (MQ=255) aaCAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAg > 1:688216/1‑140 (MQ=255) aCAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGa > 1:77022/1‑140 (MQ=255) aGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GGTG‑GGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGatc > 1:833470/1‑140 (MQ=255) cGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAGAAACCCGATGCGCAGatcat > 1:712070/1‑140 (MQ=255) cGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGatcct > 1:417667/1‑138 (MQ=255) cGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGatcat > 1:388336/1‑140 (MQ=255) aCTCCGCGGATACGGTTAATCATAAGGCTCCTTCTGAAAAACAAGTTGATCTCGTTATCGGCAAGGG‑G‑GGGG‑GG‑AAC‑CTTTTTTGTTGTCGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCACATCATCggg < 2:833470/140‑1 (MQ=255) agcgcgGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGtt > 2:555356/3‑140 (MQ=255) gcgGATACGTTAAATCATAAGCCCCCTTCCTAAAAACAAGTTGATCTCGTTACCGGAAA‑GG‑G‑GGGG‑GGAAAA‑TTTTTTTGCTGATGCCCCCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCAt < 2:124204/140‑1 (MQ=255) aTACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCa > 2:376843/1‑140 (MQ=255) tACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCaa > 1:896431/1‑140 (MQ=255) cGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAAtt > 1:323964/1‑140 (MQ=255) gTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGa > 1:69174/1‑140 (MQ=255) tCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGaaa > 2:500443/1‑140 (MQ=255) agcccccTCCCTAAAAACAAGTTGATCTCTTTATGCGCAG‑GG‑G‑GGGG‑GGAAAA‑TTTTATTGCTGAGGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCggg < 2:663519/135‑1 (MQ=255) aCCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGaa < 1:555356/140‑1 (MQ=255) ccTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAAt > 2:930224/1‑140 (MQ=255) ccTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAAt > 1:447932/1‑140 (MQ=255) ccTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAAt > 2:645685/1‑140 (MQ=255) ccTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAAt < 1:500443/140‑1 (MQ=255) tCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTAc > 1:267407/1‑140 (MQ=255) aaaaCAAGTTGATCTCGTTATCGGCAA‑GG‑A‑GGGGAGG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCAc > 1:768091/1‑140 (MQ=255) tttGATCTCGTTATGGGCAA‑GG‑A‑GGGGAGA‑AAA‑TTTTATTGCTGATCCCCCCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGAc < 2:768091/139‑1 (MQ=255) ttctcTTTACCGGAAA‑GG‑G‑GGGG‑GGAAAA‑TTTTTTTGCTGATGCCCCCCGCCGCGAAATTGAAATAAGAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGAcgccg < 2:712070/139‑1 (MQ=255) aTCTCGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGAcgccg < 2:388336/140‑1 (MQ=255) aTCTCGTTACCGGAAG‑GG‑G‑GGGG‑GGAAAA‑TTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGAcgccg < 2:447932/140‑1 (MQ=255) tctcGTTAGGGGCAG‑GG‑G‑GGGG‑GG‑AAATTTTTATTGTTGATCCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGAcgccgc < 2:236284/140‑1 (MQ=255) ctcGTTATCGGCAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCcgcg < 1:645685/140‑1 (MQ=255) agacagaTCGGAAA‑GG‑G‑GGGG‑GGAAAA‑TTTTATTGCTGATGCCCCCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCcgcg < 2:28896/134‑1 (MQ=255) cAA‑GG‑AGGGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAg > 2:64790/1‑140 (MQ=255) | AAGAAAAAAACAGACTGCCGGAAATAAGCTGTAACAGTGCGAATACCCCCAGCACCATTACCAACAGCGTGACTACGCGGATACGGTTAATCATAAGGCACCTTCCTGAAAACAAGTTGATCTCGTTATCGGCAA‑GG‑A‑GGGG‑GG‑AAA‑CTTTATTGCTGATGCCACCCGCCGCGAAATTGAAATAAAAAACCCGATGCGCAGATCATCGGGTTCATTTCAATTGAGGAAATCGGGAGAATTACGCCACTTCTGACGCCGCGCTATCTAACAG > NC_000913/1972598‑1972868 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |