Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,077,174	(G)5→6	coding (63/369 nt)	cbeA →	CP4‑44 prophage; cytoskeleton bundling‑enhancing antitoxin CbeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,077,169	1	.	G	100.0%	143.8 / NA	41	W20G (TGG→GGG)	cbeA	CP4‑44 prophage; cytoskeleton bundling‑enhancing antitoxin CbeA
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (20/21);  total (20/21)

CTGAACTGAGTGGGAATTCTGATGAGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGT‑G‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTCAGCGATGCAGATGCGTACCACCTGGA  >  NC_000913/2077030‑2077299                                                                                                                                              |                                                                                                                                    cTGAACTGAGTGGGAATTCTGATGAGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCtggt                                                                                                                                      <  2:927620/140‑1 (MQ=255)       tGAGTGGGAATTCTGATGAGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGT‑g‑ggggg                                                                                                                              <  2:268001/140‑2 (MQ=255)       tGAGTGGGAATTCTGATGAGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGT‑g‑ggggg                                                                                                                              <  2:678697/140‑2 (MQ=255)       tGAGTGGGAATTCTGATGAGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGT‑g‑ggggg                                                                                                                              <  2:682054/140‑2 (MQ=255)        gAGTGGGAATTCTGATGAGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGT‑g‑gggggc                                                                                                                             >  2:914144/1‑138 (MQ=255)         aGTGGGAATTCTGATGAGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGT‑g‑gggggct                                                                                                                            >  1:794110/1‑137 (MQ=255)         aGTGGGAATTCTGATGAGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGT‑g‑ggggg                                                                                                                              >  1:912298/1‑137 (MQ=255)         aGTGGGAATTCTGATGAGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGT‑g‑ggggg                                                                                                                              <  2:287459/138‑2 (MQ=255)         aGTGGGAATTCTGATGAGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGT‑g‑ggggg                                                                                                                              <  2:912298/138‑2 (MQ=255)         aGTGGGAATTCTGATGAGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGT‑g‑ggggg                                                                                                                              >  1:287459/1‑137 (MQ=255)          gTGGGAATTCTGATGAGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGT‑g‑gggggctg                                                                                                                           >  2:807452/1‑136 (MQ=255)          gTGGGAATTCTGATGAGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGT‑g‑gggggctg                                                                                                                           >  2:14580/1‑136 (MQ=255)                 ttCTGATGAGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCa                                                                                                                     <  2:189714/140‑1 (MQ=255)                   cTGATGAGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCAcc                                                                                                                   >  2:705039/1‑140 (MQ=255)                            aGAATTATCGCCACTACCGGCCCATTCTTAGCCGTTTTTCTGTAAGGATTTTATCGCGTAAGACAC‑ACTGCCCGGGACAACGCTTCCCGACGACAATCCCGGCCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGccc                                                                                                          <  2:480706/140‑1 (MQ=255)                                 tATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGttt                                                                                                     <  2:914701/140‑1 (MQ=255)                                      ccACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCgggg                                                                                                <  1:1367/140‑1 (MQ=255)                                       cACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGc                                                                                               <  1:924010/140‑1 (MQ=255)                                          tACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGc                                                                                               <  2:93542/137‑1 (MQ=255)                                          tACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGc                                                                                               >  1:93542/1‑137 (MQ=255)                                          tACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACg                                                                                            >  2:207697/1‑140 (MQ=255)                                           aCCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGt                                                                                           <  1:14580/140‑1 (MQ=255)                                            ccGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTc                                                                                          <  2:794110/140‑1 (MQ=255)                                               gACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTgg                                                                                       >  1:235954/1‑140 (MQ=255)                                                    ttCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCag                                                                                  >  1:932547/1‑140 (MQ=255)                                                       ttAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCaggag                                                                               >  1:641458/1‑140 (MQ=255)                                                         aGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAggg                                                                             <  1:807452/140‑1 (MQ=255)                                                              aTTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAAcc                                                                        >  1:477896/1‑140 (MQ=255)                                                               ttttCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCATGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCg                                                                       <  1:554385/140‑1 (MQ=255)                                                                tttCTGTAAGGATTTTATCGTGTCAGACAC‑CCTCCCCGGGACAACTCTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCgg                                                                      <  2:249916/140‑1 (MQ=255)                                                                          gATTTTATCGTGTCAG‑CACGGCGCCCCGGGACAACCCTTCTCGACTCCAATCACATTCACCCCTGGG‑GCGGGCCTGCCGTGCCCCGTAACGCCCTGTTTCGGGGCACGTGTGGTGCAGCAGGGTAACCGATTGAATTAcc                                                            <  2:842855/140‑1 (MQ=255)                                                                             ttttTCGTGTCAGACAC‑CCCCCCCGGGACAACCCTCCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGGCGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTg                                                         <  2:477896/140‑1 (MQ=255)                                                                             tttATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTg                                                         >  2:531465/1‑140 (MQ=255)                                                                                    tgtCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACcg                                                  >  2:257408/1‑140 (MQ=255)                                                                                      tCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACcgcg                                                <  2:932547/140‑1 (MQ=255)                                                                                      tCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACcgcg                                                <  1:411718/140‑1 (MQ=255)                                                                                         gACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCg                                             <  1:531465/140‑1 (MQ=255)                                                                                               acTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATc                                        >  2:816942/1‑140 (MQ=255)                                                                                                cTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCa                                       <  1:675265/140‑1 (MQ=255)                                                                                                cTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCa                                       >  2:675265/1‑140 (MQ=255)                                                                                                   cccGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCagag                                    >  2:437911/1‑140 (MQ=255)                                                                                                   cccGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCagag                                    >  2:741091/1‑140 (MQ=255)                                                                                                        gACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTg                               >  1:582215/1‑140 (MQ=255)                                                                                                          caacaCTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGtt                             <  1:207697/140‑1 (MQ=255)                                                                                                           aacaCTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTc                            >  2:433181/1‑140 (MQ=255)                                                                                                             cacTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCagag                                    >  1:171110/1‑130 (MQ=255)                                                                                                             cacTTCCCGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCagag                                    <  2:171110/130‑1 (MQ=255)                                                                                                                  cccGACGACAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTCAGCGATg                     >  1:906929/1‑140 (MQ=255)                                                                                                                     gacgacAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTCAGCGATGCAg                  <  1:433181/140‑1 (MQ=255)                                                                                                                      acgacAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTCAGCGATGCAGa                 <  1:493853/140‑1 (MQ=255)                                                                                                                          cAATCACGACCGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTCAGCGATGCAGATGCg             >  2:785231/1‑140 (MQ=255)                                                                                                                                    cGCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTCAGCGATGCAGATGCGTACCACCTgg   >  2:524592/1‑140 (MQ=255)                                                                                                                                     gCCCCTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTCAGCGATGCAGATGCGTACCACCTGGa  <  2:235954/140‑1 (MQ=255)                                                                                                                                        ccTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTCAGCGATGCAGATGCGt            <  1:271321/127‑1 (MQ=255)                                                                                                                                        ccTGGTGG‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTCAGCGATGCAGATGCGt            >  2:271321/1‑127 (MQ=255)                                                                                                                                              |                                                                                                                                    CTGAACTGAGTGGGAATTCTGATGAGCAGAATTATCGCCACTACCGGACCATTCTTAGCCGATTTTCTGTAAGGATTTTATCGTGTCAGACAC‑ACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGT‑G‑GGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTCAGCGATGCAGATGCGTACCACCTGGA  >  NC_000913/2077030‑2077299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑