Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,242,2340CT42.0% 23.6 / 57.8 50G361S (GGC→AGC) uxaAD‑altronate dehydratase
Reads supporting (aligned to +/- strand):  ref base C (13/16);  new base T (9/12);  total (22/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.84e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CAGCCCGCACCCGCCAGGGCGCTGGTCGCTACGGCATCGTTACCCGGCGCACTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCT  >  NC_000913/3242095‑3242370
                                                                                                                                           |                                                                                                                                        
cAGCCCGCACCCGCCAGGGCGCTGGTCGCTACGGCATCGTTACCCGGCGCACTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGcc                                                                                                                                          >  2:866662/1‑140 (MQ=255)
   cccGCACCCGCCAGGGCGCTGGTCGCTACGGCATCGTTACCCGGCGCACTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAg                                                                                                                                       >  2:216014/1‑140 (MQ=255)
     cGCACCCGCCAGGGCGCTGGTCGCTACGGCATCGTTACCCGGCGCACTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCTAAGTg                                                                                                                                     <  2:328262/140‑1 (MQ=255)
     cGCACCCGCCAGGGCGCTGGTCGCTACGGCATCGTTACCCGGCGCACTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTg                                                                                                                                     >  2:942651/1‑140 (MQ=255)
     cGCACCCGCCAGGGCGCTGGTCGCTACGGCATCGTTACCCGGCGCACTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTg                                                                                                                                     <  1:906284/140‑1 (MQ=255)
     cGCACCCGCCAGGGCGCTGGTCGCTACGGCATCGTTACCCGGCGCACTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTg                                                                                                                                     >  2:731234/1‑140 (MQ=255)
         cccGCCAGGGCGCTGGTCGCTACGGCATCGTTACCCGGCGCACTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCTAAGTGAttt                                                                                                                                 <  2:592162/140‑1 (MQ=255)
         cccGCCAGGGCGCTGGTCGCTACGGCATCGTTACCCGGCGCACTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGAttt                                                                                                                                 <  1:758190/140‑1 (MQ=255)
              cAGGGCGCTGGTCGCTACGGCATCGTTACCCGGCGCACTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCtt                                                                                                                            <  1:731234/140‑1 (MQ=255)
               aGGGCGCTGGTCGCTACGGCATCGTTACCCGGCGCACTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTc                                                                                                                           <  1:39611/140‑1 (MQ=255)
                gggCGCTGGTCGCTACGGCATCGTTACCCGGCGCACTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCTAAGTGATTTGTCTTcc                                                                                                                          <  2:770052/140‑1 (MQ=255)
                gggCGCTGGTCGCTACGGCATCGTTACCCGGCGCACTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTcc                                                                                                                          <  2:884910/140‑1 (MQ=255)
                  gcgcTGGTCGCTACGGCATCGTTACCCGGCGCACTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCTAAGTGATTTGTCTTCCAg                                                                                                                        <  2:228789/140‑1 (MQ=255)
                  gcgcTGGTCGCTACGGCATCGTTACCCGGCGCACTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCTAAGTGATTTGTCTTCCAg                                                                                                                        <  1:468662/140‑1 (MQ=255)
                  gcgcTGGTCGCTACGGCATCGTTACCCGGCGCACTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAg                                                                                                                        <  1:521321/140‑1 (MQ=255)
                  gcgcTGGTCGCTACGGCATCGTTACCCGGCGCACTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAg                                                                                                                        <  1:866662/140‑1 (MQ=255)
                                    tCGTTACCCGGCGCACTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCTAAGTGATTTGTCTTCCAGCGTGGTGATAccgcccgc                                                                                                      >  1:483633/1‑140 (MQ=255)
                                               cgcACTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCTAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCg                                                                                           >  2:609694/1‑140 (MQ=255)
                                               cgcACTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCg                                                                                           >  2:464970/1‑140 (MQ=255)
                                               cgcACTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCg                                                                                           >  2:249893/1‑140 (MQ=255)
                                               cgcACTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCg                                                                                           >  1:581672/1‑140 (MQ=255)
                                                   cTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCTAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGa                                                                                       <  2:587139/140‑1 (MQ=255)
                                                   cTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGa                                                                                       <  1:249893/140‑1 (MQ=255)
                                                   cTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGa                                                                                       <  1:464970/140‑1 (MQ=255)
                                                    ttAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGAt                                                                                      >  1:388843/1‑140 (MQ=255)
                                                       aCAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCTAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATggg                                                                                   <  1:496943/140‑1 (MQ=255)
                                                       aCAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCTAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATggg                                                                                   <  2:390406/140‑1 (MQ=255)
                                                       aCAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATggg                                                                                   <  1:216014/140‑1 (MQ=255)
                                                             tCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCg                                                                                        >  1:841183/1‑129 (MQ=255)
                                                             tCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCg                                                                                        <  2:841183/129‑1 (MQ=255)
                                                              cAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCTAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCAt                                                                            >  2:741779/1‑140 (MQ=255)
                                                              cAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCTGCGATGGGTTTTCAt                                                                            >  2:626990/1‑140 (MQ=255)
                                                              cAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCAt                                                                            >  2:381690/1‑140 (MQ=255)
                                                                gCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCTAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAg                                                                          >  2:604089/1‑140 (MQ=255)
                                                                 cccTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGa                                                                         >  1:898373/1‑140 (MQ=255)
                                                                 cccTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGa                                                                         <  2:388843/140‑1 (MQ=255)
                                                                  ccTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCTAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGAt                                                                        >  1:778934/1‑140 (MQ=255)
                                                                  ccTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGAt                                                                        <  2:898373/140‑1 (MQ=255)
                                                                     ggCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCTAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCgg                                                                     <  1:277697/140‑1 (MQ=255)
                                                                     ggCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCTAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCgg                                                                     >  2:917547/1‑140 (MQ=255)
                                                                      gCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCTAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGc                                                                    >  1:763529/1‑140 (MQ=255)
                                                                           ttCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCTAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATc                                                               <  1:604089/140‑1 (MQ=255)
                                                                              aGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCTAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATg                                                            <  1:206209/140‑1 (MQ=255)
                                                                                aCGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCTGCGATGGGTTTTCATAGATCGGCTGATCATggg                                                          <  1:626990/140‑1 (MQ=255)
                                                                                                      tCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCTAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTc                                    <  2:763529/140‑1 (MQ=255)
                                                                                                          ccACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTg                                >  1:774230/1‑140 (MQ=255)
                                                                                                              gacgCTGGAACCCGCTTTCTGGGTACAGCTAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCa                            >  1:213129/1‑140 (MQ=255)
                                                                                                                      aaCCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCATGGTGAcc                    <  2:774230/140‑1 (MQ=255)
                                                                                                                         ccGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCATGGTGACCAGt                 <  1:381690/140‑1 (MQ=255)
                                                                                                                          cGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCATGGTGACCAGtt                >  1:38486/1‑140 (MQ=255)
                                                                                                                                        aGCTAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCt  >  1:323612/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                        
CAGCCCGCACCCGCCAGGGCGCTGGTCGCTACGGCATCGTTACCCGGCGCACTTAACAAGTTCAGCCCTGGCGTTTTCAGACGCTCGCCGTAACGCAGCACGTCAACCACGACGCTGGAACCCGCTTTCTGGGTACAGCCAAGTGATTTGTCTTCCAGCGTGGTGATACCGCCCGCTTTGTTCCCCGGCGATGGGTTTTCATAGATCGGCTGATCATGGGCAATAAAGTACTGTTTGAAGTCGTTGACCATGGTGACCAGTTTTTCAAACGTTGCT  >  NC_000913/3242095‑3242370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑