Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,596,4650TC71.4% 61.0 / 27.8 42T366A (ACG→GCG) livKL‑leucine/L‑phenylalanine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (6/6);  new base C (16/14);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CGTAGGTACTGCCCAGCGTGACGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCA  >  NC_000913/3596337‑3596601
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cgTAGGTACTGCCCAGCGTGACGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGCGGATGAACCGt                                                                                                                               >  1:537944/1‑140 (MQ=255)
  tAGGTACTGCCCAGCGTGACGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGCGGATGAACCGTCg                                                                                                                             <  1:635559/140‑1 (MQ=255)
    ggTACTGCCCAGCGTGACGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGCGGATGAACCGTCGGc                                                                                                                           <  2:34269/140‑1 (MQ=255)
     gTACTGCCCAGCGTGACGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGCGGATGAACCGTCGGCg                                                                                                                          <  2:537944/140‑1 (MQ=255)
      tACTGCCCAGCGTGACGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGt                                                                                                                         <  2:424781/140‑1 (MQ=255)
                cGTGACGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGCGGATGAACCGTCGGCGTGCCACTGGaa                                                                                                               >  2:210979/1‑140 (MQ=255)
                  tGACGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGCGGATGAACCGTCGGCGTGCCa                                                                                                                     <  1:691048/132‑1 (MQ=255)
                  tGACGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGCGGATGAACCGTCGGCGTGCCa                                                                                                                     >  2:691048/1‑132 (MQ=255)
                     cGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGacac                                                                                                          >  1:497965/1‑140 (MQ=255)
                          ttAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGCGGATGAACCGTCGGCGTGCCACTGGAAGACACCaaaa                                                                                                     >  2:329721/1‑140 (MQ=255)
                              aCATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGCGGATGAACCGTCGGCGTGCCACTGGAAGACACCAaaatcaa                                                                                                 <  1:281334/140‑1 (MQ=255)
                               cATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAGCCGTCGGCGTGCCACTGGAAGACACCAaaatcaaa                                                                                                <  2:12730/140‑1 (MQ=255)
                               cATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGCGGATGAACCGTCGGCGTGCCACTGGAAGACACCAaaatcaaa                                                                                                <  1:398281/140‑1 (MQ=255)
                                 tCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGCGGATGAACCGTCGGCGTGCCACTGGAAGACACCAaaatcaaatc                                                                                              <  1:329721/140‑1 (MQ=255)
                                    gctgcAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCt                                                                                           >  1:308279/1‑140 (MQ=255)
                                    gctgcAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGCGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCt                                                                                           >  1:720903/1‑140 (MQ=255)
                                    gctgcAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGCGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCt                                                                                           >  2:832398/1‑140 (MQ=255)
                                          aGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGAt                                                                                     >  1:388683/1‑140 (MQ=255)
                                                   aaaaCTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCttttt                                                                            <  2:388683/140‑1 (MQ=255)
                                                         gCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATccc                                                                      >  2:101994/1‑140 (MQ=255)
                                                                         aCCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGCGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAAt                                                      >  2:891959/1‑140 (MQ=255)
                                                                            tttCTCAACCCGCCCGCATTTTACGGCCGGTGGGATGATCACTTGGCTGCCGCGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCAc                                                   <  2:851559/140‑1 (MQ=255)
                                                                             ttCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGCGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACg                                                  <  2:720903/140‑1 (MQ=255)
                                                                             ttCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGCGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACg                                                  >  1:604845/1‑140 (MQ=255)
                                                                              tCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCaa                                                       >  2:298074/1‑134 (MQ=255)
                                                                              tCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCaa                                                       <  1:298074/134‑1 (MQ=255)
                                                                              tCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGCGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACgg                                                 <  1:210979/140‑1 (MQ=255)
                                                                                        cccgcATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACTGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCAcc                                       >  2:350764/1‑140 (MQ=255)
                                                                                           gcATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGtt                                    <  2:497965/140‑1 (MQ=255)
                                                                                           gcATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGCGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGtt                                    <  1:625323/140‑1 (MQ=255)
                                                                                                   cGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGTTAGCTTTTa                            <  1:454523/140‑1 (MQ=255)
                                                                                                         gTGGGATGATCACTTGGCTGCCGCGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCtt                      >  2:870870/1‑140 (MQ=255)
                                                                                                           gggATGATCACTTGGCTGCCGCGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGTTAGCTTTTaaa                          >  1:525294/1‑134 (MQ=255)
                                                                                                           gggATGATCACTTGGCTGCCGCGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGTTAGCTTTTaaa                          <  2:525294/134‑1 (MQ=255)
                                                                                                           gggATGATCACTTGGCTGCCGCGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGTTAGCTTTTaaa                          <  2:842937/134‑1 (MQ=255)
                                                                                                           gggATGATCACTTGGCTGCCGCGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGTTAGCTTTTaaa                          >  1:842937/1‑134 (MQ=255)
                                                                                                           gggATGATCACTTGGCTGCCGCGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTc                    >  2:368378/1‑140 (MQ=255)
                                                                                                                    aCTTGGCTGCCGCGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCAccagcgcc           >  1:432144/1‑140 (MQ=255)
                                                                                                                      ttGGCTGCCGCGGATGAACCGTCTGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCAccagcgccag         >  2:273/1‑140 (MQ=255)
                                                                                                                        ggCTGCCGCGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCAccagcgccagcg       >  1:233541/1‑140 (MQ=255)
                                                                                                                           tGCCGCGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCt    >  2:216506/1‑140 (MQ=255)
                                                                                                                             ccGCGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCa  <  1:870870/140‑1 (MQ=255)
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CGTAGGTACTGCCCAGCGTGACGCCGTTAAACATCTGCTGCAAGAAATACAAAAACTGCTCAGACATAAGGTAACCTTTCTAAACCCGCCCGCATTTTACGGGCGGTGGGATGATCACTTGGCTGCCGTGGATGAACCGTCGGCGTGCCACTGGAAGACACCAAAATCAAATCCCTTAAGATCGCCTTTTTCATCCCAGTTCAGCGGCCCAATCACGGTGTTTGCACCGTTAGCTTTTAAATCTTTCACCAGCGCCAGCGGCTCA  >  NC_000913/3596337‑3596601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑