Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	31,923	T→C	G369G (GGT→GGC)	carB →	carbamoyl phosphate synthetase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	31,923	0	T	C	100.0%	188.2 / NA	53	G369G (GGT→GGC)	carB	carbamoyl phosphate synthetase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/27);  total (26/27)

CGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTCGCACGCAGCAGGAATCCCTGCAAAAAGCGCTGCGCGGCCTGGAAGTCGGTGCGACTGGATTCGACCCGAAAGT  >  NC_000913/31784‑32054                                                                                                                                             |                                                                                                                                    cggcgAAACTGGCGGTGGGTTACACCCTCCACGAAATGATGAACGACATCACTGGCGGGCGGACTCCCGGCCCCCTTC‑AGGCGTCCCTTGACTATTTGGGTTCTAAAAATTCTCCCTTCAACTTTGGAAAAATTGCCTGt                                                                                                                                     >  2:520089/1‑140 (MQ=255)        aCTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACg                                                                                                                              <  1:489385/140‑1 (MQ=255)            gCGGTGGGTTACCCCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCg                                                                                                                          <  2:602612/140‑1 (MQ=255)            gCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCg                                                                                                                          <  2:513776/140‑1 (MQ=255)            gCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCg                                                                                                                          <  2:627331/140‑1 (MQ=255)                 gggTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGa                                                                                                                     <  1:73831/140‑1 (MQ=255)                 gggTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGa                                                                                                                     <  1:552249/140‑1 (MQ=255)                  ggTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGAc                                                                                                                    <  1:930515/140‑1 (MQ=255)                      acacCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACt                                                                                                                >  1:694369/1‑140 (MQ=255)                      acacCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACt                                                                                                                >  2:263366/1‑140 (MQ=255)                            tCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATg                                                                                                          >  2:357144/1‑140 (MQ=255)                                 gaACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATc                                                                                                     <  1:609808/140‑1 (MQ=255)                                    cTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGt                                                                                                  <  1:384243/140‑1 (MQ=255)                                       atgaACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTgg                                                                                               >  1:779108/1‑140 (MQ=255)                                       atgaACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTgg                                                                                               >  1:228267/1‑140 (MQ=255)                                         gaACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCg                                                                                             <  1:697760/140‑1 (MQ=255)                                            cGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAg                                                                                          >  1:710877/1‑140 (MQ=255)                                               cATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCgg                                                                                                   <  2:340796/128‑1 (MQ=255)                                               cATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCgg                                                                                                   >  1:340796/1‑128 (MQ=255)                                                    cTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCg                                                                                  >  2:546936/1‑140 (MQ=255)                                                            cGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGAt                                                                                <  1:308260/134‑1 (MQ=255)                                                            cGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGAt                                                                                >  2:308260/1‑134 (MQ=255)                                                              tACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTcgca                                                                        <  1:263366/140‑1 (MQ=255)                                                              tACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTcgca                                                                        <  1:546936/140‑1 (MQ=255)                                                              tACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTcgca                                                                        <  1:820862/140‑1 (MQ=255)                                                               aCT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTcgcac                                                                       >  2:554790/1‑140 (MQ=255)                                                                      gCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTcgca                                                                        >  2:326261/1‑133 (MQ=255)                                                                      gCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTcgca                                                                        <  1:326261/133‑1 (MQ=255)                                                                      gCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTCGCACgcagca                                                                 >  1:856467/1‑140 (MQ=255)                                                                      gCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTCGCACgcagca                                                                 >  2:233196/1‑140 (MQ=255)                                                                        ctcctTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTCGCACGCAGCAgg                                                               >  2:341671/1‑140 (MQ=255)                                                                         tcctTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTcgca                                                                        <  2:653163/130‑1 (MQ=255)                                                                         tcctTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTcgca                                                                        >  1:653163/1‑130 (MQ=255)                                                                                aGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTCGCACGCAGCAGGAATCCCTg                                                       >  2:828423/1‑140 (MQ=255)                                                                                aGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTCGCACGCAGCAGGAATCCCTg                                                       >  2:930369/1‑140 (MQ=255)                                                                                     tCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTCGCACGCAGCAGGAATCCCTGCaaaa                                                  >  2:422815/1‑140 (MQ=255)                                                                                           gACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTCGCACGCAGCAGGAATCCCTGCAAAAAGCGCt                                            <  2:856467/140‑1 (MQ=255)                                                                                              tATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTCGCACGCAGCAGGAATCCCTGCAAAAAGCGCTgcg                                         <  1:828423/140‑1 (MQ=255)                                                                                              tATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTCGCACGCAGCAGGAATCCCTGCAAAAAGCGCTgcg                                         <  1:357144/140‑1 (MQ=255)                                                                                              tATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTCGCACGCAGCAGGAATCCCTGCAAAAAGCGCTgcg                                         <  1:341671/140‑1 (MQ=255)                                                                                                       aCTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTCGCACGCAGCAGGAATCCCTGCAAAAAGCGCTGCGCGGCCTGGa                                >  2:327890/1‑140 (MQ=255)                                                                                                       aCTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTCGCACGCAGCAGGAATCCCTGCAAAAAGCGCTGCGCGGCCTGGa                                >  1:355034/1‑140 (MQ=255)                                                                                                          aaaaTTCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTCGCACGCAGCAGGAATCCCTGCAAAAAGCGCTGCGCGGCCTGGAAGt                             >  1:233033/1‑140 (MQ=255)                                                                                                              ttCCTCGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTCGCACGCAGCAGGAATCCCTGCAAAAAGCGCTGCGCGGCCTGGAAGTCGGt                         >  1:207382/1‑140 (MQ=255)                                                                                                                   cGCTTCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTCGCACGCAGCAGGAATCCCTGCAAAAAGCGCTGCGCGGCCTGGAAGTCGGTGCGAc                    <  1:327890/140‑1 (MQ=255)                                                                                                                      ttCAACTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTCGCACGCAGCAGGAATCCCTGCAAAAAGCGCTGCGCGGCCTGGAAGTCGGTGCGACTgg                 >  1:1989/1‑140 (MQ=255)                                                                                                                         aaCTTCGAAAAATTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTCGCACGCAGCAGGAATCCCTGCAAAAAGCGCTGCGCGGCCTGGAAGTCGGTGCGACTGGAtt              <  2:694369/140‑1 (MQ=255)                                                                                                                                aaaaaTTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTCGCACGCAGCAGGAATCCCTGCAAAAAGCGCTGCGCGGCCTGGAAGTCGGTGCGAc                    <  1:953162/127‑1 (MQ=255)                                                                                                                                aaaaaTTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTCGCACGCAGCAGGAATCCCTGCAAAAAGCGCTGCGCGGCCTGGAAGTCGGTGCGAc                    >  2:953162/1‑127 (MQ=255)                                                                                                                                aaaaaTTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTCGCACGCAGCAGGAATCCCTGCAAAAAGCGCTGCGCGGCCTGGAAGTCGGTGCGACTGGATTCGACCCg       >  2:941796/1‑140 (MQ=255)                                                                                                                                aaaaaTTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATAGGTTGGCGAAGTGATGGCGATTGGTCGCACGCAGCAGGAATCCCTGCAAAAAGCGCTGCGCGGCCTGGAAGTCGGTGCGACTGGATTCGACCCg       >  1:168286/1‑140 (MQ=255)                                                                                                                                    aTTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTCGCACGCAGCAGGAATCCCTGCAAAAAGCGCTGCGCGGCCTGGAAGTCGGTGCGACTGGATTCGACCCGAAAg   <  2:355034/140‑1 (MQ=255)                                                                                                                                    aTTCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTCGCACGCAGCAGGAATCCCTGCAAAAAGCGCTGCGCGGCCTGGAAGTCGGTGCGACTGGATTCGACCCGAAAg   <  1:488648/140‑1 (MQ=255)                                                                                                                                     ttCGCCGGCGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTCGCACGCAGCAGGAATCCCTGCAAAAAGCGCTGCGCGGCCTGGAAGTCGGTGCGACTGGATTCGACCCGAAAGt  <  2:1989/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                                    CGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACT‑CCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATGAAATCGGTTGGCGAAGTGATGGCGATTGGTCGCACGCAGCAGGAATCCCTGCAAAAAGCGCTGCGCGGCCTGGAAGTCGGTGCGACTGGATTCGACCCGAAAGT  >  NC_000913/31784‑32054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑