Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	268,692	T→A	D90V (GAT→GTT)	ykfA ←	CP4‑6 prophage; putative GTP‑binding protein YkfA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	268,692	0	T	A	100.0%	261.7 / NA	75	D90V (GAT→GTT)	ykfA	CP4‑6 prophage; putative GTP‑binding protein YkfA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (37/38);  total (37/38)

AAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACA  >  NC_000913/268555‑268823                                                                                                                                          |                                                                                                                                    aaaaaTGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAAcc                                                                                                                                   <  2:402920/140‑1 (MQ=255)   aaaTGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCAt                                                                                                                                 <  2:399886/140‑1 (MQ=255)   aaaTGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCAt                                                                                                                                 >  2:584857/1‑140 (MQ=255)     aTGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCa                                                                                                                               <  1:892654/140‑1 (MQ=255)     aTGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCa                                                                                                                               <  1:584857/140‑1 (MQ=255)      tGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAg                                                                                                                              >  1:832656/1‑140 (MQ=255)      tGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAg                                                                                                                              <  2:773448/140‑1 (MQ=255)        ttCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCg                                                                                                                            <  2:347667/140‑1 (MQ=255)        ttCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCg                                                                                                                            >  1:281741/1‑140 (MQ=255)          cATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCa                                                                                                                               <  2:310763/135‑1 (MQ=255)          cATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCa                                                                                                                               >  1:310763/1‑135 (MQ=255)            tCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTtat                                                                                                                        >  2:114683/1‑140 (MQ=255)                ccGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCAt                                                                                                                                 <  2:563129/127‑1 (MQ=255)                ccGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCAt                                                                                                                                 >  1:563129/1‑127 (MQ=255)                 cGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGc                                                                                                                   >  2:906321/1‑140 (MQ=255)                   tCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCgg                                                                                                                 >  2:352863/1‑140 (MQ=255)                       cgcCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGc                                                                                                             >  2:735356/1‑140 (MQ=255)                       cgcCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGc                                                                                                             >  1:642388/1‑140 (MQ=255)                         cccGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCg                                                                                                           <  1:735356/140‑1 (MQ=255)                           cGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGAc                                                                                                         >  1:796304/1‑140 (MQ=255)                             gTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACtt                                                                                                       >  1:398412/1‑140 (MQ=255)                                        tAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAg                                                                                            <  2:195694/140‑1 (MQ=255)                                           tCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAgcgc                                                                                         <  1:687906/140‑1 (MQ=255)                                                cacaGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTgg                                                                                    <  2:281655/140‑1 (MQ=255)                                                cacaGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTgg                                                                                    <  2:266188/140‑1 (MQ=255)                                                cacaGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTgg                                                                                    <  1:114683/140‑1 (MQ=255)                                                cacaGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTgg                                                                                    <  1:843226/140‑1 (MQ=255)                                                   aGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTc                                                                                 <  1:606993/140‑1 (MQ=255)                                                   aGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTc                                                                                 >  1:191217/1‑140 (MQ=255)                                                   aGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTc                                                                                 >  2:799534/1‑140 (MQ=255)                                                     tACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCAc                                                                               <  2:876871/140‑1 (MQ=255)                                                          ggTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCTGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTa                                                                          >  2:594493/1‑140 (MQ=255)                                                            tCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACa                                                                        <  2:832656/140‑1 (MQ=255)                                                            tCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACa                                                                        >  2:8430/1‑140 (MQ=255)                                                            tCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACa                                                                        >  1:920933/1‑140 (MQ=255)                                                               aggcgaggAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGgc                                                                     >  2:714385/1‑140 (MQ=255)                                                               aggcgaggAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGgc                                                                     >  1:100719/1‑140 (MQ=255)                                                               aggcgaggAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGgc                                                                     >  1:354973/1‑140 (MQ=255)                                                                     ggAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCcac                                                               >  2:231013/1‑140 (MQ=255)                                                                            gTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTg                                                        >  1:320527/1‑140 (MQ=255)                                                                             ttCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGa                                                       <  1:839228/140‑1 (MQ=255)                                                                              tCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGAc                                                      <  2:234159/140‑1 (MQ=255)                                                                              tCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGAc                                                      <  1:231013/140‑1 (MQ=255)                                                                              tCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGAc                                                      <  2:100719/140‑1 (MQ=255)                                                                              tCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGAc                                                      <  1:906321/140‑1 (MQ=255)                                                                               cgcgGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGAcc                                                     <  1:799534/140‑1 (MQ=255)                                                                                 cgGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCTGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCgg                                                   <  1:594493/140‑1 (MQ=255)                                                                                       aGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGatat                                             <  2:908070/140‑1 (MQ=255)                                                                                         cgcAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGatatat                                           >  2:107881/1‑140 (MQ=255)                                                                                         cgcAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGatatat                                           >  1:13780/1‑140 (MQ=255)                                                                                         cgcAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGatatat                                           >  2:239774/1‑140 (MQ=255)                                                                                              caTACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCg                                                    >  2:557793/1‑126 (MQ=255)                                                                                              caTACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCg                                                    <  1:557793/126‑1 (MQ=255)                                                                                              caTACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCg                                      >  1:809917/1‑140 (MQ=255)                                                                                              caTACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCg                                      >  2:674773/1‑140 (MQ=255)                                                                                                   tCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCaaa                                 >  1:218255/1‑140 (MQ=255)                                                                                                     ggTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACa                               <  1:107881/140‑1 (MQ=255)                                                                                                        aTCGCGAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAggg                            >  1:181608/1‑140 (MQ=255)                                                                                                         tCGCGAGCGCCACTTTCGCCCACGCCCGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGc                           <  1:674773/140‑1 (MQ=255)                                                                                                          cgcgAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCa                          <  2:326812/140‑1 (MQ=255)                                                                                                            cgAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGc                                    >  2:339377/1‑128 (MQ=255)                                                                                                            cgAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGc                                    <  1:339377/128‑1 (MQ=255)                                                                                                            cgAGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCAtt                        <  2:13780/140‑1 (MQ=255)                                                                                                              aGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGc                      >  2:635798/1‑140 (MQ=255)                                                                                                              aGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGc                      <  2:642388/140‑1 (MQ=255)                                                                                                              aGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGc                      <  2:20969/140‑1 (MQ=255)                                                                                                              aGCGCCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGc                      <  2:316075/140‑1 (MQ=255)                                                                                                               gcgcCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAgg                             >  2:286656/1‑132 (MQ=255)                                                                                                               gcgcCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAgg                             <  1:286656/132‑1 (MQ=255)                                                                                                                 gcCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGcaca                   >  2:687432/1‑140 (MQ=255)                                                                                                                 gcCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGcaca                   >  1:772274/1‑140 (MQ=255)                                                                                                                 gcCACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGcaca                   >  2:353739/1‑140 (MQ=255)                                                                                                                   cACTTTCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGa                 >  1:149372/1‑140 (MQ=255)                                                                                                                        tCGCCCACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGct            <  1:643084/140‑1 (MQ=255)                                                                                                                            ccACGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTc        <  2:281741/140‑1 (MQ=255)                                                                                                                               cGCCGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAATCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCa     >  2:701648/1‑140 (MQ=255)                                                                                                                                  cGGGCAGAACCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAaca  <  1:239774/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    AAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGGCAAACAGGGCATTGCACAGACTGCTCTTCCCAACA  >  NC_000913/268555‑268823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑