Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,232,517	G→A	G225D (GGC→GAC)	cdd →	cytidine/deoxycytidine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,232,517	0	G	A	100.0%	158.7 / NA	46	G225D (GGC→GAC)	cdd	cytidine/deoxycytidine deaminase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/20);  total (26/20)

CAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATGCGCCGTTG  >  NC_000913/2232381‑2232653                                                                                                                                         |                                                                                                                                         cAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAg                                                                                                                                       <  1:237722/140‑1 (MQ=255)     aTCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTAc                                                                                                                                   >  1:196920/1‑140 (MQ=255)            cTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGaaa                                                                                                                            <  1:688048/140‑1 (MQ=255)              aTGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAAc                                                                                                                          >  2:488337/1‑140 (MQ=255)               tGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAAcg                                                                                                                         >  1:853707/1‑140 (MQ=255)                    tGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCa                                                                                                                    >  2:630687/1‑140 (MQ=255)                      aCGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCAtt                                                                                                                  <  1:630687/140‑1 (MQ=255)                           tGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAAcc                                                                                                             >  2:164644/1‑140 (MQ=255)                           tGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAAcc                                                                                                             >  1:271220/1‑140 (MQ=255)                           tGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAAcc                                                                                                             <  2:858585/140‑1 (MQ=255)                              tGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGa                                                                                                          >  2:148516/1‑140 (MQ=255)                              tGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGa                                                                                                          >  1:944658/1‑140 (MQ=255)                                cgcTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGAct                                                                                                        >  2:91579/1‑140 (MQ=255)                                        cAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCAcc                                                                                                <  2:271220/140‑1 (MQ=255)                                             aGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGc                                                                                           >  1:433375/1‑140 (MQ=255)                                               cGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAg                                                                                         <  2:433375/140‑1 (MQ=255)                                               cGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAg                                                                                         >  2:721417/1‑140 (MQ=255)                                               cGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAg                                                                                         >  1:31089/1‑140 (MQ=255)                                                gATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAgg                                                                                        <  2:853707/140‑1 (MQ=255)                                                gATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAgg                                                                                        <  2:944658/140‑1 (MQ=255)                                                 aTTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAggg                                                                                       >  1:253212/1‑140 (MQ=255)                                                           cAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAAtt                                                                             >  2:587920/1‑140 (MQ=255)                                                                 gTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTg                                                                       <  2:485858/140‑1 (MQ=255)                                                                    cGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAAt                                                                    <  1:164644/140‑1 (MQ=255)                                                                        caTGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCa                                                                <  1:488337/140‑1 (MQ=255)                                                                        caTGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCa                                                                <  2:31089/140‑1 (MQ=255)                                                                         aTGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCaa                                                               <  1:399980/140‑1 (MQ=255)                                                                         aTGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCaa                                                               <  2:381288/140‑1 (MQ=255)                                                                                 cAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATg                                                       >  1:901446/1‑140 (MQ=255)                                                                                   gTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGAt                                                     >  1:254360/1‑140 (MQ=255)                                                                                      aGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGAtt                                                    >  1:386498/1‑138 (MQ=255)                                                                                      aGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGAtt                                                    <  2:386498/138‑1 (MQ=255)                                                                                      aGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTa                                                   <  1:581873/139‑1 (MQ=255)                                                                                      aGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTa                                                   >  2:581873/1‑139 (MQ=255)                                                                                      aGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTAc                                                  >  1:431955/1‑140 (MQ=255)                                                                                         cGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTAcc                                                 <  2:958565/138‑1 (MQ=255)                                                                                         cGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTAcc                                                 >  1:958565/1‑138 (MQ=255)                                                                                         cGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCg                                               >  1:475515/1‑140 (MQ=255)                                                                                               gCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATc                                         <  2:901446/140‑1 (MQ=255)                                                                                                  gtgtCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAg                                      >  1:924332/1‑140 (MQ=255)                                                                                                     tCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAgcgc                                   <  1:410332/140‑1 (MQ=255)                                                                                                      cgcgCTGGAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAgcgcg                                  >  1:370668/1‑140 (MQ=255)                                                                                                             gAATGTAAAGACGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCt                           >  1:440128/1‑140 (MQ=255)                                                                                                                       aCGGTCGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGaaaaa                 >  1:474412/1‑140 (MQ=255)                                                                                                                            cGTATTTTCAGTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGa            >  2:755920/1‑140 (MQ=255)                                                                                                                                      gTGACAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATGCGCCGTTg  <  1:587920/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATGCGCCGTTG  >  NC_000913/2232381‑2232653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑