Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,449,457	T→C	K198R (AAG→AGG)	yfcO ←	DUF2544 domain‑containing protein YfcO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,449,457	0	T	C	100.0%	202.9 / NA	57	K198R (AAG→AGG)	yfcO	DUF2544 domain‑containing protein YfcO
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/28);  total (29/28)

GTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGG  >  NC_000913/2449320‑2449590                                                                                                                                          |                                                                                                                                      gTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTc                                                                                                                                     >  2:47480/1‑140 (MQ=255)  tttGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTcc                                                                                                                                    <  2:269512/140‑1 (MQ=255)    tGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCa                                                                                                                                  >  2:444980/1‑140 (MQ=255)          ttATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTtc                                                                                                                            <  2:790918/140‑1 (MQ=255)            ataGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTtcat                                                                                                                          <  2:621692/140‑1 (MQ=255)                ggTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCaaa                                                                                                                      >  1:216672/1‑140 (MQ=255)                   gtCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATAc                                                                                                                   >  1:613357/1‑140 (MQ=255)                    tCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACg                                                                                                                  >  1:636517/1‑140 (MQ=255)                    tCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACg                                                                                                                  >  2:701265/1‑140 (MQ=255)                     cGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGt                                                                                                                 >  1:598700/1‑140 (MQ=255)                       aTTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTg                                                                                                               <  2:174265/140‑1 (MQ=255)                          tGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGaaa                                                                                                            >  2:60044/1‑140 (MQ=255)                            gCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGc                                                                                                          <  1:104221/140‑1 (MQ=255)                             cGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCg                                                                                                         <  1:47480/140‑1 (MQ=255)                             cGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCg                                                                                                         <  2:793647/140‑1 (MQ=255)                                     agaCTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCa                                                                                                 <  2:216672/140‑1 (MQ=255)                                           ccGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACcaca                                                                                           >  2:566265/1‑140 (MQ=255)                                           ccGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACcaca                                                                                           >  2:925017/1‑140 (MQ=255)                                            cGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCacac                                                                                          <  1:925017/140‑1 (MQ=255)                                               cTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGc                                                                                       <  2:156654/140‑1 (MQ=255)                                                 tGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGccc                                                                                     <  1:566265/140‑1 (MQ=255)                                                        tCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACt                                                                              >  1:61349/1‑140 (MQ=255)                                                        tCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACt                                                                              <  1:67258/140‑1 (MQ=255)                                                          ccGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGc                                                                            <  1:411015/140‑1 (MQ=255)                                                          ccGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGc                                                                            <  2:930486/140‑1 (MQ=255)                                                            gACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAg                                                                          >  2:336102/1‑140 (MQ=255)                                                             aCGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAgg                                                                         >  1:23211/1‑140 (MQ=255)                                                             aCGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAgg                                                                         >  2:104479/1‑140 (MQ=255)                                                              cgcgGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAATCGTTTTCTCAACCACACCGCCCATCGACTGCAggg                                                                        >  2:678805/1‑140 (MQ=255)                                                                 ggTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGa                                                                     <  2:598700/140‑1 (MQ=255)                                                                  gTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAg                                                                    <  1:104479/140‑1 (MQ=255)                                                                  gTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAg                                                                    <  1:60044/140‑1 (MQ=255)                                                                   tAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGc                                                                   >  1:773874/1‑140 (MQ=255)                                                                   tAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGc                                                                   <  1:188163/140‑1 (MQ=255)                                                                           atcatcTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACCGCAGGGTGAGCAGTTTTGa                                                           >  2:146328/1‑140 (MQ=255)                                                                                cTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTAc                                                      <  1:444980/140‑1 (MQ=255)                                                                                 tgtgCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACt                                                     >  2:247275/1‑140 (MQ=255)                                                                                   tgCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGa                                                   >  1:632678/1‑140 (MQ=255)                                                                                    gCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGAc                                                  <  1:230413/140‑1 (MQ=255)                                                                                        cccAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATaa                                              >  2:786390/1‑140 (MQ=255)                                                                                           aGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATtc                                           >  2:295366/1‑140 (MQ=255)                                                                                           aGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATtc                                           <  1:247275/140‑1 (MQ=255)                                                                                            gCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATtct                                          <  2:23211/140‑1 (MQ=255)                                                                                                  gTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTAc                                                      <  2:131648/122‑1 (MQ=255)                                                                                                  gTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTAc                                                      >  1:131648/1‑122 (MQ=255)                                                                                                  gTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTcc                                    >  1:116600/1‑140 (MQ=255)                                                                                                     accaccTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATg                                 >  2:28983/1‑140 (MQ=255)                                                                                                      ccaccTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACCGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGa                                <  1:146328/140‑1 (MQ=255)                                                                                                        accTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGaaa                              <  1:336102/140‑1 (MQ=255)                                                                                                        accTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGaaa                              <  2:773874/140‑1 (MQ=255)                                                                                                        accTGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGaaa                              <  1:791177/140‑1 (MQ=255)                                                                                                           tGTTTCATTCATCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGt                           >  2:696851/1‑140 (MQ=255)                                                                                                                  ttcatCTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAgg                    <  2:61349/140‑1 (MQ=255)                                                                                                                       cTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGaa               >  2:571768/1‑140 (MQ=255)                                                                                                                       cTGCGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGaa               >  1:162510/1‑140 (MQ=255)                                                                                                                          cGTACTGTCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAg            >  1:51315/1‑140 (MQ=255)                                                                                                                                 tCGCACAACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGa     >  2:867414/1‑140 (MQ=255)                                                                                                                                    cacaACCTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATgg  >  1:128337/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      GTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTCCGCCTTGTCACATCCCGACGCGGTAAAAGTTATCATCTGTGCAACCCAGCGGCAGTAACCACCTGTTTCATTCATCTGCGTACTGTCGCACAACTTCCCATCTTTCATCAAATACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGG  >  NC_000913/2449320‑2449590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑