Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,590,374	T→C	V927A (GTG→GCG)	acrD →	multidrug efflux pump RND permease AcrD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,590,374	0	T	C	100.0%	177.2 / NA	53	V927A (GTG→GCG)	acrD	multidrug efflux pump RND permease AcrD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/29);  total (24/29)

TTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGTGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTAACGAGATGAACCAAAAAGGCCACGACCTGTTTGAAGCGACGCTCCACGCCTGCCGTCAGCGTTTACGCCCG  >  NC_000913/2590241‑2590507                                                                                                                                      |                                                                                                                                      ttCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATAGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGc                                                                                                                                   <  2:517484/138‑1 (MQ=255) ttCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATAGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGc                                                                                                                                   >  1:517484/1‑138 (MQ=255)     tgtgTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTa                                                                                                                             <  2:873059/140‑1 (MQ=255)      gtgtCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTaa                                                                                                                            <  2:188135/140‑1 (MQ=255)        gtCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACg                                                                                                                          >  1:821719/1‑140 (MQ=255)           tGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTc                                                                                                                       >  1:877512/1‑140 (MQ=255)           tGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTc                                                                                                                       <  2:496090/140‑1 (MQ=255)           tGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTc                                                                                                                       >  1:558661/1‑140 (MQ=255)            ggCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTaa                                                                                                                            >  1:244203/1‑134 (MQ=255)            ggCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTaa                                                                                                                            <  2:244203/134‑1 (MQ=255)            ggCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCa                                                                                                                      >  1:44730/1‑140 (MQ=255)              cTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCAtt                                                                                                                    >  2:655572/1‑140 (MQ=255)              cTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCAtt                                                                                                                    >  2:742360/1‑140 (MQ=255)                    tGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTa                                                                                                              >  1:782833/1‑140 (MQ=255)                            aGCTGGTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGaa                                                                                                      <  1:93779/140‑1 (MQ=255)                            aGCTGGTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGaa                                                                                                      <  2:697115/140‑1 (MQ=255)                            aGCTGGTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGaa                                                                                                      <  2:44730/140‑1 (MQ=255)                                ggTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAAc                                                                                                  >  1:619966/1‑140 (MQ=255)                                ggTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAAc                                                                                                  >  1:113715/1‑140 (MQ=255)                                ggTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAAc                                                                                                  >  1:453963/1‑140 (MQ=255)                                 gTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACg                                                                                                 <  2:619966/140‑1 (MQ=255)                                 gTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACg                                                                                                 <  2:113715/140‑1 (MQ=255)                                      tGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATc                                                                                            >  2:311056/1‑140 (MQ=255)                                        ccGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCt                                                                                          >  2:747289/1‑140 (MQ=255)                                        ccGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCCGTTAACCGTCTTTGGTTTAACGGCGAAAAACGAcctcct                                                                                          >  2:467856/1‑140 (MQ=255)                                                ggTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGAtcgtcg                                                                                  >  1:818590/1‑140 (MQ=255)                                                ggTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGAtcg                                                                                     >  1:331268/1‑137 (MQ=255)                                                ggTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGAtcg                                                                                     <  2:331268/137‑1 (MQ=255)                                                    aTGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGtt                                                                              <  2:821719/140‑1 (MQ=255)                                                     tGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGttt                                                                             >  2:159961/1‑140 (MQ=255)                                                     tGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGttt                                                                             >  1:695408/1‑140 (MQ=255)                                                          gTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTaa                                                                        >  1:689585/1‑140 (MQ=255)                                                          gTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTaa                                                                        <  2:689585/140‑1 (MQ=255)                                                          gTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTaa                                                                        <  1:159961/140‑1 (MQ=255)                                                          gTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTa                                                                         >  1:410954/1‑139 (MQ=255)                                                          gTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTa                                                                         <  2:410954/139‑1 (MQ=255)                                                            cGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTAACg                                                                      >  2:165758/1‑140 (MQ=255)                                                            cGTGCCGCTGGGGGCAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGGCTGTTAACGGGCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTAACg                                                                      >  2:49758/1‑140 (MQ=255)                                                              tGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTAACgag                                                                    <  1:165758/140‑1 (MQ=255)                                                              tGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTAACgag                                                                    <  1:742360/140‑1 (MQ=255)                                                                ccGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTAACGAGAt                                                                  <  2:877512/140‑1 (MQ=255)                                                                ccGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTAACGAGAt                                                                  <  2:558661/140‑1 (MQ=255)                                                                  gCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTAACGAGATGa                                                                <  2:453963/140‑1 (MQ=255)                                                                     gggggTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTAACGAGATGAAcc                                                             >  1:30821/1‑140 (MQ=255)                                                                                   cgcTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTAACGAGATGAACCAAAAAGGCCACGAc                                               <  2:818590/140‑1 (MQ=255)                                                                                                cTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTAACGAGATGAACCAAAAAGGCCACGACCTGTTTGAAGCGa                                  <  1:655572/140‑1 (MQ=255)                                                                                                cTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTAACGAGATGAACCAAAAAGGCCACGACCTGTTTGAAGCGa                                  <  2:30821/140‑1 (MQ=255)                                                                                                   gATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTAACGAGATGAACCAAAAAGGCCACGACCTGTTTGAAGCGACGc                               <  2:782833/140‑1 (MQ=255)                                                                                                         cgGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTAACGAGATGAACCAAAAAGGCCACGACCTGTTTGAAGCGa                                  <  2:622052/131‑1 (MQ=255)                                                                                                         cgGGCTGGAAAACGACGTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTAACGAGATGAACCAAAAAGGCCACGACCTGTTTGAAGCGa                                  >  1:622052/1‑131 (MQ=255)                                                                                                                      gacgTTTACTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTAACGAGATGAACCAAAAAGGCCACGACCTGTTTGAAGCGACGCTCCACGCCTGCCGTCAGCg            >  2:552443/1‑140 (MQ=255)                                                                                                                              cTTCCAGGCGGGTCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTAACGAGATGAACCAAAAAGGCCACGACCTGTTTGAAGCGACGCTCCACGCCTGCCGTCAGCGTTTACGcc    <  1:467856/140‑1 (MQ=255)                                                                                                                              cTTCCAGGCGGGGCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTAACGAGATGAACCAAAAAGGCCACGACCTGTTTGAAGCGACGCTCCACGCCTGCCGTCAGCGTTTACGcc    <  1:49758/140‑1 (MQ=255)                                                                                                                              cTTCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTAACGAGATGAACCAAAAAGGCCACGACCTGTTTGAAGCGACGCTCCACGCCTGCCGTCAGCGTTTACGcc    <  1:747289/140‑1 (MQ=255)                                                                                                                                tCCAGGCGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTAACGAGATGAACCAAAAAGGCCACGACCTGTTTGAAGCGACGCTCCACGCCTGCCGTCAGCGTTTACGCCCg  <  2:330023/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      TTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTAATGCTGGTCGTGCCGCTGGGGGTAATCGGCGCGCTGCTGGCAACCTGGATGCGCGGGCTGGAAAACGACGTTTACTTCCAGGTGGGCCTGTTAACGGTCATTGGTTTATCGGCGAAAAACGCCATCCTGATCGTCGAGTTTGCTAACGAGATGAACCAAAAAGGCCACGACCTGTTTGAAGCGACGCTCCACGCCTGCCGTCAGCGTTTACGCCCG  >  NC_000913/2590241‑2590507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑