Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,602,233	T→C	V139A (GTG→GCG)	hyfB →	hydrogenase 4 component B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,602,233	0	T	C	100.0%	253.2 / NA	73	V139A (GTG→GCG)	hyfB	hydrogenase 4 component B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/37);  total (36/37)

TGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCC  >  NC_000913/2602100‑2602360                                                                                                                                      |                                                                                                                                tGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGtt                                                                                                                           >  1:126931/1‑140 (MQ=255)   ttCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTg                                                                                                                         >  1:825017/1‑140 (MQ=255)         ctATTCATTGACTTATATGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAatga                                                                                                                   >  1:738363/1‑140 (MQ=255)            ttCATTGACTTATATGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGt                                                                                                                <  2:126931/140‑1 (MQ=255)             tCATTGACTTATATGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTc                                                                                                               >  2:97014/1‑140 (MQ=255)             tCATTGACTTATATGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTc                                                                                                               >  2:860772/1‑140 (MQ=255)                ttGACTTATATGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCt                                                                                                            <  1:743706/140‑1 (MQ=255)                 tGACTTATATGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTg                                                                                                           >  2:322898/1‑140 (MQ=255)                 tGACTTATATGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTg                                                                                                           <  2:363503/140‑1 (MQ=255)                    cTTATATGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCt                                                                                                        >  1:100739/1‑140 (MQ=255)                    cTTATATGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCt                                                                                                        >  2:483279/1‑140 (MQ=255)                    cTTATATGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCt                                                                                                        >  2:713866/1‑140 (MQ=255)                      tataTGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGaaa                                                                                                                      >  2:847469/1‑124 (MQ=255)                      tataTGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGaaa                                                                                                                      <  1:847469/124‑1 (MQ=255)                      tataTGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTc                                                                                                      >  2:655943/1‑140 (MQ=255)                      tataTGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTc                                                                                                      >  2:597298/1‑140 (MQ=255)                      tataTGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTc                                                                                                      >  1:741713/1‑140 (MQ=255)                        tatGCGCGAATACGAGTGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCt                                                                                                    >  2:307199/1‑140 (MQ=255)                        tatGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCt                                                                                                    <  1:597298/140‑1 (MQ=255)                          tGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTgg                                                                                                  >  2:525292/1‑140 (MQ=255)                           gcgcGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGt                                                                                                 >  1:338258/1‑140 (MQ=255)                              cgAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTc                                                                                              >  2:262303/1‑140 (MQ=255)                               gAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCt                                                                                             >  1:639121/1‑140 (MQ=255)                                 aTACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTc                                                                                                         <  1:529227/126‑1 (MQ=255)                                 aTACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTc                                                                                                         >  2:529227/1‑126 (MQ=255)                                  tACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGt                                                                                          <  2:920723/140‑1 (MQ=255)                                  tACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCCTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGCGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGt                                                                                          >  2:482804/1‑140 (MQ=255)                                       gggCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGt                                                                                          <  2:575331/135‑1 (MQ=255)                                       gggCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGt                                                                                          >  1:575331/1‑135 (MQ=255)                                       gggCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTg                                                                                     <  1:322898/140‑1 (MQ=255)                                           aaaGGCGCGGCGGCGATGGGCTCCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCcag                                                                                 <  1:681089/140‑1 (MQ=255)                                            aaGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGt                                                                                          <  1:504015/130‑1 (MQ=255)                                            aaGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGt                                                                                          >  2:504015/1‑130 (MQ=255)                                             aGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCcaggc                                                                               >  2:960339/1‑140 (MQ=255)                                                 gcggcggcgATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGa                                                                           <  2:100739/140‑1 (MQ=255)                                                         gATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGt                                                                   <  2:738363/140‑1 (MQ=255)                                                          aTGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTc                                                                  >  1:224474/1‑140 (MQ=255)                                                             ggCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGAt                                                               <  1:97014/140‑1 (MQ=255)                                                             ggCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGAt                                                               <  1:262303/140‑1 (MQ=255)                                                             ggCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGAt                                                               <  1:960339/140‑1 (MQ=255)                                                             ggCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGAt                                                               <  1:860772/140‑1 (MQ=255)                                                             ggCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGAt                                                               <  1:483279/140‑1 (MQ=255)                                                                ttcttTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCaa                                                            <  2:741713/140‑1 (MQ=255)                                                                ttcttTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCaa                                                            <  2:224474/140‑1 (MQ=255)                                                                  cttTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACg                                                          <  1:525292/140‑1 (MQ=255)                                                                     tATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTAGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTg                                                       <  1:655943/140‑1 (MQ=255)                                                                      aTGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTgg                                                      >  2:152642/1‑140 (MQ=255)                                                                            atTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGct                                                >  2:701712/1‑140 (MQ=255)                                                                             ttttCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGctc                                               >  2:707743/1‑140 (MQ=255)                                                                              tttCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTg                                                       <  2:808573/131‑1 (MQ=255)                                                                              tttCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTg                                                       >  1:808573/1‑131 (MQ=255)                                                                               ttCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTa                                             >  2:367015/1‑140 (MQ=255)                                                                                  atcgcatcgATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACtt                                          <  1:307199/140‑1 (MQ=255)                                                                                      catcgATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACtttttt                                      <  1:612519/140‑1 (MQ=255)                                                                                       atcgATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTa                                     >  1:506524/1‑140 (MQ=255)                                                                                       atcgATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTa                                     <  1:701712/140‑1 (MQ=255)                                                                                           aTGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGc                                 >  2:854373/1‑140 (MQ=255)                                                                                                     tgctgGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGa                       >  1:372407/1‑140 (MQ=255)                                                                                                      gctgGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGAt                      <  2:506524/140‑1 (MQ=255)                                                                                                      gctgGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGAt                      <  2:372407/140‑1 (MQ=255)                                                                                                       ctgGTGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATc                     >  2:627877/1‑140 (MQ=255)                                                                                                           tGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTg                 <  2:338258/140‑1 (MQ=255)                                                                                                           tGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTg                 <  1:482804/140‑1 (MQ=255)                                                                                                           tGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTg                 <  1:849079/140‑1 (MQ=255)                                                                                                           tGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTg                 <  1:854373/140‑1 (MQ=255)                                                                                                           tGATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTg                 <  2:825017/140‑1 (MQ=255)                                                                                                           ggATGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTGCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTg                 <  2:639121/139‑1 (MQ=255)                                                                                                             aTGGACAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCt               >  1:518136/1‑140 (MQ=255)                                                                                                                 aCAACGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATa           >  2:849477/1‑140 (MQ=255)                                                                                                                     cGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTg              >  2:394925/1‑133 (MQ=255)                                                                                                                     cGCTTTTTGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTg              <  1:394925/133‑1 (MQ=255)                                                                                                                        tttttGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCg    <  1:707743/140‑1 (MQ=255)                                                                                                                          tttGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGAt      <  2:388154/136‑1 (MQ=255)                                                                                                                          tttGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGAt      >  1:388154/1‑136 (MQ=255)                                                                                                                          tttGGTTCATCGCGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGcc  <  1:627877/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                TGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCAAAGGCGCGGCGGCGATGGGCTTCTTTATGAATATTTTCATCGCATCGATGGTTGCCCTGCTGGTGATGGACAACGCTTTTTGGTTCATCGTGCTGTTTGAAATGATGTCGCTGTCTTCCTGGTTTCTGGTCATTGCCAGGCAGGATAAAACGTCGATCAACGCTGGCATGCTCTACTTTTTTATCGCCCACGCCGGATCGGTGCTGATAATGATCGCC  >  NC_000913/2602100‑2602360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑