Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,603,102	G→A	G429S (GGT→AGT)	hyfB →	hydrogenase 4 component B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,603,102	0	G	A	100.0%	209.4 / NA	59	G429S (GGT→AGT)	hyfB	hydrogenase 4 component B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/32);  total (27/32)

CGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGGGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCA  >  NC_000913/2602972‑2603230                                                                                                                                   |                                                                                                                                 cGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTg                                                                                                                         <  2:78204/140‑1 (MQ=255)  gcagcaTGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGc                                                                                                                        <  1:146772/140‑1 (MQ=255)  gcagcaTGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGc                                                                                                                        <  2:847449/140‑1 (MQ=255)   cagcaTGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCt                                                                                                                       <  1:588999/140‑1 (MQ=255)     gcaTGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTAt                                                                                                                     >  1:460156/1‑140 (MQ=255)        tGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGt                                                                                                                  <  2:460156/140‑1 (MQ=255)        tGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGt                                                                                                                  <  1:120193/140‑1 (MQ=255)          ccTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTaa                                                                                                                >  2:89980/1‑140 (MQ=255)          ccTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTaa                                                                                                                >  2:289811/1‑140 (MQ=255)              aTTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTAt                                                                                                                           <  2:723571/125‑1 (MQ=255)              aTTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTAt                                                                                                                           >  1:723571/1‑125 (MQ=255)                    tGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGt                                                                                                      <  2:335859/140‑1 (MQ=255)                          gcgATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACtgg                                                                                                >  2:16536/1‑140 (MQ=255)                               atCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGc                                                                                           <  1:413947/140‑1 (MQ=255)                                  aGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTgg                                                                                        <  1:168879/140‑1 (MQ=255)                                  aGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTgg                                                                                        <  1:894987/140‑1 (MQ=255)                                  aGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTgg                                                                                        <  2:233143/140‑1 (MQ=255)                                    ccATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCa                                                                                      <  1:16536/140‑1 (MQ=255)                                    ccATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCa                                                                                      >  1:658515/1‑140 (MQ=255)                                        tcctccGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGc                                                                                           <  1:534858/131‑1 (MQ=255)                                        tcctccGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGc                                                                                           >  2:534858/1‑131 (MQ=255)                                        tcctccGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTaa                                                                                  >  1:94430/1‑140 (MQ=255)                                        tcctccGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTaa                                                                                  >  2:710317/1‑140 (MQ=255)                                              gCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCt                                                                            >  1:5503/1‑140 (MQ=255)                                               cTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCtt                                                                           <  2:253440/140‑1 (MQ=255)                                                  aaTGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGcctgtc                                                                        >  1:833284/1‑135 (MQ=255)                                                   aTGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTa                                                                       <  1:646325/140‑1 (MQ=255)                                                    tGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAAtgtg                                                                              >  2:458858/1‑132 (MQ=255)                                                    tGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAAtgtg                                                                              <  1:458858/132‑1 (MQ=255)                                                          tATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGt                                                                <  1:525922/140‑1 (MQ=255)                                                                              ggCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTa                                                          <  1:609753/126‑1 (MQ=255)                                                                              ggCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTa                                                          >  2:609753/1‑126 (MQ=255)                                                                               gCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTAtt                                                        <  2:938221/127‑1 (MQ=255)                                                                               gCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTAtt                                                        >  1:938221/1‑127 (MQ=255)                                                                               gCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTATTACTTTCTgtggtg                                           >  1:61166/1‑140 (MQ=255)                                                                                 aGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGt                                                           <  1:294681/122‑1 (MQ=255)                                                                                 aGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGt                                                           >  2:294681/1‑122 (MQ=255)                                                                                   tCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTATTACTTTCTGTGGTGcgcc                                       >  1:379816/1‑140 (MQ=255)                                                                                   tCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTATTACTTTCTGTGGTGcgcc                                       >  2:371239/1‑140 (MQ=255)                                                                                     gCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTATTACTTTCTGTGGTGcgccgc                                     >  1:658148/1‑140 (MQ=255)                                                                                      cTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTATTACTTTCTGTGGTGcgcc                                       >  1:680244/1‑137 (MQ=255)                                                                                      cTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTATTACTTTCTGTGGTGcgcc                                       <  2:680244/137‑1 (MQ=255)                                                                                       tGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTATTACTTTCTGTGGTGCGCCgcgc                                   <  2:379816/140‑1 (MQ=255)                                                                                        gTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTATTACTTTCTGTGGTGcgccgc                                     >  1:257977/1‑137 (MQ=255)                                                                                        gTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTATTACTTTCTGTGGTGcgccgc                                     <  2:257977/137‑1 (MQ=255)                                                                                            tcACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTATTACTTTCTGTGGTGCGCCGCGCAGTac                              <  1:89980/140‑1 (MQ=255)                                                                                             cACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTATTACTTTCTGTGGTGCGCcgcg                                    >  2:151011/1‑133 (MQ=255)                                                                                             cACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTATTACTTTCTGTGGTGCGCcgcg                                    <  1:151011/133‑1 (MQ=255)                                                                                                   gTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCt                       >  2:802098/1‑140 (MQ=255)                                                                                                   gTCGTGTGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCt                       >  1:842494/1‑140 (MQ=255)                                                                                                      gtgtGGAAGCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGaa                    <  2:658148/140‑1 (MQ=255)                                                                                                              gCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTATTACTTTCTGTGGTGCGCCGCGCAGTac                              <  1:526396/122‑1 (MQ=255)                                                                                                              gCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTATTACTTTCTGTGGTGCGCCGCGCAGTac                              >  2:526396/1‑122 (MQ=255)                                                                                                              gCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGaa                    >  2:88603/1‑132 (MQ=255)                                                                                                              gCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGaa                    <  1:88603/132‑1 (MQ=255)                                                                                                              gCCGTAGCGCTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGcaca            >  1:289448/1‑140 (MQ=255)                                                                                                                       cTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGcc   <  1:710317/140‑1 (MQ=255)                                                                                                                       cTACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGcc   <  1:802098/140‑1 (MQ=255)                                                                                                                        tACAACTTGCGAGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCa  <  1:289811/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                 CGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACTAAGTCGTGTGGAAGCCGTAGCGCTACAACTTGCGGGTCCTATTGCTATGGTAATGCTGGCAGTCACTGGTGGGCTGGCAGTAATGTGCTTCGTAAAAATGTACGGTATTACTTTCTGTGGTGCGCCGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCA  >  NC_000913/2602972‑2603230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑