Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,706,770	C→T	A119T (GCC→ACC)	lepA ←	elongation factor 4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,706,770	0	C	T	100.0%	119.5 / NA	36	A119T (GCC→ACC)	lepA	elongation factor 4
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/19);  total (17/19)

CGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGCGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCTACGTGGCCCGGGGTGTCGATAAAGTTA  >  NC_000913/2706636‑2706906                                                                                                                                       |                                                                                                                                         cGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGgtgt                                                                                                                                     >  1:206213/1‑140 (MQ=255)  gCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTa                                                                                                                                    >  1:731700/1‑140 (MQ=255)       aCAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGt                                                                                                                               >  1:447040/1‑140 (MQ=255)       aCAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGt                                                                                                                               <  2:419268/140‑1 (MQ=255)                     cGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTgc                                                                                                                 >  2:818414/1‑140 (MQ=255)                     cGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTgc                                                                                                                 <  2:194654/140‑1 (MQ=255)                      ggTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTgcg                                                                                                                <  2:206213/140‑1 (MQ=255)                      ggTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTgcg                                                                                                                <  1:818414/140‑1 (MQ=255)                             tCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTAc                                                                                                         >  2:616078/1‑140 (MQ=255)                                aTGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGcc                                                                                                      <  2:164000/140‑1 (MQ=255)                                    cgacgaTATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGc                                                                                                  >  2:826091/1‑140 (MQ=255)                                        gaTATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCgg                                                                                              <  2:731700/140‑1 (MQ=255)                                                cAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGacc                                                                                      <  1:826091/140‑1 (MQ=255)                                                 aaTTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGacca                                                                                     >  2:941588/1‑140 (MQ=255)                                                   tttCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGaccacc                                                                                   >  2:613063/1‑140 (MQ=255)                                                        tcCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCaa                                                                              >  1:375925/1‑140 (MQ=255)                                                               cgcgTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCt                                                                       >  2:469135/1‑140 (MQ=255)                                                                    tCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTcaca                                                                  <  2:447040/140‑1 (MQ=255)                                                                            ggCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGcagcca                                                          <  1:590738/140‑1 (MQ=255)                                                                             gCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGcagccag                                                         >  1:903176/1‑140 (MQ=255)                                                                              cTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGcagccagc                                                        <  2:929193/140‑1 (MQ=255)                                                                                  cGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAAc                                                    >  1:215130/1‑140 (MQ=255)                                                                                  cGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAAc                                                    >  2:316698/1‑140 (MQ=255)                                                                                        gtaaaTCTTGTTCAGTGTCGGCAGAACCTCGAGATCCATTTCCATGGTGGTGTGGAAGTTTGCCAGGGTTTGCGGTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGTCAGGGATCGGGaaa                                              <  2:141070/137‑1 (MQ=255)                                                                                                tGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAg                                      <  2:215130/140‑1 (MQ=255)                                                                                                tGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAg                                      <  1:279866/140‑1 (MQ=255)                                                                                                 gTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAgg                                     <  1:941588/140‑1 (MQ=255)                                                                                                 gTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAgg                                     <  1:469135/140‑1 (MQ=255)                                                                                                      gTACCGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAg                                <  1:613063/140‑1 (MQ=255)                                                                                                         ccGGCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCt                             >  2:334848/1‑140 (MQ=255)                                                                                                            gCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGga                                    >  1:488318/1‑130 (MQ=255)                                                                                                            gCACAACTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGga                                    <  2:488318/130‑1 (MQ=255)                                                                                                                 aCTTCGAGATCCATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCTACGTGGcc                     >  2:86822/1‑140 (MQ=255)                                                                                                                            cATTTCCATGGTGGTGTAGTAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCTAc                           <  2:461836/123‑1 (MQ=255)                                                                                                                            cATTTCCATGGTGGTGTAGTAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCTAc                           >  1:461836/1‑123 (MQ=255)                                                                                                                            cATTTCCATGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCTACGTGGCCCGGGGTGTCGa          <  2:375925/140‑1 (MQ=255)                                                                                                                                   aTGGTGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCTACGTGGCCCGGGGTGTCGATAAAGtt   <  1:86822/140‑1 (MQ=255)                                                                                                                                    tggtggTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCTACGTGGCCCGGGGTGTCGATAAAGTTa  >  1:881107/1‑140 (MQ=255)                                                                                                                                    tggtggTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCTACGTGGCCCGGGGTGTCGATAAAGTTa  <  2:881107/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         CGCTGAACAGCGCACCGCGTCGGTGGCGTCGATGCCGACGATATCTTCAATTTCTTCCGCCACGCGTTCAGGATCGGCTGCCGGCAGGTCAATCTTGTTCAGTACCGGCACAACTTCGAGATCCATTTCCATGGCGGTGTAGCAGTTTGCCAGGGTTTGCGCTTCTACGCCCTGCCCGGCGTCGACCACCAGCAATGCACCTTCACAGGCAGCCAGCGAACGGGAAACTTCATAGGAGAAGTCTACGTGGCCCGGGGTGTCGATAAAGTTA  >  NC_000913/2706636‑2706906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑