Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,715,149	T→C	T58A (ACC→GCC)	yfiE ←	putative LysR‑type DNA‑binding transcriptional regulator YfiE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,715,149	0	T	C	100.0%	109.3 / NA	31	T58A (ACC→GCC)	yfiE	putative LysR‑type DNA‑binding transcriptional regulator YfiE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/12);  total (19/12)

CAACGTTTCGCCCGAAACAACGCGTAGTTCCCCGTCCGGATCTGACTCCTTTTTGGCAGCCTCACGAAGCGTATCCATCACTCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAAC  >  NC_000913/2715021‑2715274                                                                                                                                 |                                                                                                                              cAACGTTTCGCCCGAAACAACGCGTAGTTCCCCGTCCGGATCTGACTCCTTTTTGGCAGCCTCACGAAGCGTATCCATCACTCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGCAAGGCACATcc                                                                                                                    >  2:677573/1‑140 (MQ=255) cAACGTTTCGCCCGAAACAACGCGTAGTTCCCCGTCCGGATCTGACTCCTTTTTGGCAGCCTCACGAAGCGTATCCATCACTCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGCAAGGCACATcc                                                                                                                    >  2:757766/1‑140 (MQ=255)  aaCGTTTCGCCCGAAACAACGCGTAGTTCCCCGTCCGGATCTGACTCCTTTTTGGCAGCCTCACGAAGCGTATCCATCACTCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGCAAGGCACATCCg                                                                                                                   <  1:388610/140‑1 (MQ=255)      tttCGCCCGAAACAACGCGTAGTTCCCCGTCCGGATCTAACTCCTTTTTGGCAGCCTCACGAAGCGTATCCATCACTCGGGTAAGTTCATAAATGTGCGGCAGCAACTTGTTTCCTTCGCGGGCCAGGCACATCCGTAgg                                                                                                               >  2:590265/1‑140 (MQ=255)                       cgTAGTTCCCCGTCCGGATCTGACTCCTTTTTGGCAGCCTCACGAAGCGTATCCATCACTCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATaa                                                                                              <  2:620428/140‑1 (MQ=255)                          aGTTCCCCGTCCGGATCTGACTCCTTTTTGGCAGCCTCACGAAGCGTATCCATCACTCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTg                                                                                           >  2:266284/1‑140 (MQ=255)                                     cGGATCTGACTCCTTTTTGGCAGCCTCACGAAGCGTATCCATCACTCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACt                                                                                <  1:266284/140‑1 (MQ=255)                                     cGGATCTGACTCCTTTTTGGCAGCCTCACGAAGCGTATCCATCACTCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACt                                                                                <  1:677573/140‑1 (MQ=255)                                             aCTCCTTTTTGGCAGCCTCACGAAGCGTATCCATCACTCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCa                                                                        >  2:611313/1‑140 (MQ=255)                                                   ttttGGCAGCCTCACGAAGCGTATCCATCACTCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAgctgc                                                                  <  2:748050/140‑1 (MQ=255)                                                       ggCAGCCTCACGAAGCGTATCCATCACTCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGaa                                                              >  2:173138/1‑140 (MQ=255)                                                                aCGAAGCGTATCCATCACTCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAg                                                     <  1:898671/140‑1 (MQ=255)                                                                     gCGTATCCATCACTCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCAcc                                                >  2:550979/1‑140 (MQ=255)                                                                        tATCCATCACTCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGt                                              >  2:529454/1‑139 (MQ=255)                                                                        tATCCATCACTCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGt                                              <  1:529454/139‑1 (MQ=255)                                                                                aCTCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTgtgt                                     >  1:192997/1‑140 (MQ=255)                                                                                  tCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCAcc                                                >  2:190325/1‑127 (MQ=255)                                                                                  tCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCAcc                                                <  1:190325/127‑1 (MQ=255)                                                                                  tCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTa                                    >  1:62066/1‑139 (MQ=255)                                                                                  tCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTa                                    <  2:62066/139‑1 (MQ=255)                                                                                              aTAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAAttttt                       >  2:541666/1‑140 (MQ=255)                                                                                                     tgCGGCAGCAACTTTTTTCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGc                >  1:748875/1‑140 (MQ=255)                                                                                                     tgCGGCAGCAACTTTTTTCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGc                >  2:812169/1‑140 (MQ=255)                                                                                                     tgCGGCAGCAACTTTTTTCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGc                >  2:514893/1‑140 (MQ=255)                                                                                                     tgCGGCAGCAACTTTTTTCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGc                >  1:361006/1‑140 (MQ=255)                                                                                                     tgCGGCAGCAACTTTTTTCCTTCGCGGACAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGc                >  2:9074/1‑140 (MQ=255)                                                                                                       cGGCAGCAACTTTTTTCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTc              >  2:789488/1‑140 (MQ=255)                                                                                                          cagcaACTTTTTTCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCa           <  1:541666/140‑1 (MQ=255)                                                                                                          cagcaACTTTTTTCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCa           >  1:392393/1‑140 (MQ=255)                                                                                                                   ttttCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAAc  <  2:192997/140‑1 (MQ=255)                                                                                                                   ttttCCTTCGCGGGCAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAAc  <  1:611313/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                              CAACGTTTCGCCCGAAACAACGCGTAGTTCCCCGTCCGGATCTGACTCCTTTTTGGCAGCCTCACGAAGCGTATCCATCACTCGGGTAAGTTCATAAATGTGCGGCAGCAACTTTTTTCCTTCGCGGGTAAGGCACATCCGTCGGCCAATTTTCTCAAATAACTGGACTGAAAACTCCTGCTCAAGCTGCTGAATATGAAAAGTCACCGTCGATTGTGTACAGCACAATTTTTGCGAAGCTCGCAAAAAGGAAC  >  NC_000913/2715021‑2715274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑