Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,839,566	G→A	A15T (GCA→ACA)	ascF →	beta‑glucoside specific PTS enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,839,566	0	G	A	100.0%	234.0 / NA	67	A15T (GCA→ACA)	ascF	beta‑glucoside specific PTS enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/37);  total (30/37)

TTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGGCACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGT  >  NC_000913/2839436‑2839700                                                                                                                                   |                                                                                                                                       ttCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGggcg                                                                                                                               <  1:742432/140‑1 (MQ=255)    ttAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGgcggcg                                                                                                                            <  1:714488/140‑1 (MQ=255)    ttAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGgcggcg                                                                                                                            <  2:668156/140‑1 (MQ=255)          ccGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGGGATAGCGACACTGGGCGGCGTTGATa                                                                                                                      >  2:933220/1‑140 (MQ=255)             tttCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACa                                                                                                                   >  1:591925/1‑140 (MQ=255)             tttCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATCCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACa                                                                                                                   >  2:340316/1‑140 (MQ=255)                 aGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACAtctc                                                                                                               >  1:313955/1‑140 (MQ=255)                     tCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTcggcg                                                                                                           >  2:806616/1‑140 (MQ=255)                     tCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTcggcg                                                                                                           <  1:706927/140‑1 (MQ=255)                      cGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTcggcgg                                                                                                          <  2:313955/140‑1 (MQ=255)                        gCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGTGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTc                                                                                                        <  1:360476/140‑1 (MQ=255)                        gCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGt                                                                                                         >  2:943762/1‑139 (MQ=255)                        gCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGt                                                                                                         <  1:943762/139‑1 (MQ=255)                           tttttCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACg                                                                                                     <  2:591925/140‑1 (MQ=255)                           tttttCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACg                                                                                                     <  2:168753/140‑1 (MQ=255)                           tttttCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACg                                                                                                     <  2:547919/140‑1 (MQ=255)                            ttttCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGc                                                                                                    <  2:732418/140‑1 (MQ=255)                             tttCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCa                                                                                                   <  2:169078/140‑1 (MQ=255)                                     aCGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGa                                                                                           <  1:547431/140‑1 (MQ=255)                                               aTAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCATGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTgcg                                                                                 <  2:168894/140‑1 (MQ=255)                                                 aacaacTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCt                                                                               >  2:887042/1‑140 (MQ=255)                                                    aacTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTg                                                                            <  2:291925/140‑1 (MQ=255)                                                      cTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGtt                                                                          >  1:149583/1‑140 (MQ=255)                                                          aTCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGtcatca                                                                      >  1:693545/1‑140 (MQ=255)                                                          aTCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCa                                                                      >  1:210045/1‑140 (MQ=255)                                                           tCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCaa                                                                     <  1:89429/140‑1 (MQ=255)                                                              aCCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAga                                                                  <  2:359859/140‑1 (MQ=255)                                                                   aCTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGatgatg                                                             <  1:887042/140‑1 (MQ=255)                                                                   aCTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGatgatg                                                             <  1:933220/140‑1 (MQ=255)                                                                   aCTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGatgatg                                                             <  2:564929/140‑1 (MQ=255)                                                                   aCTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGatgatg                                                             <  1:340316/140‑1 (MQ=255)                                                                    cTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTTCGCTTTGTTATCaa                                                                     >  1:582142/1‑131 (MQ=255)                                                                    cTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTg                                                                            <  2:475591/124‑1 (MQ=255)                                                                    cTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTg                                                                            >  1:475591/1‑124 (MQ=255)                                                                    cTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCaa                                                                     <  2:416772/131‑1 (MQ=255)                                                                    cTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCaa                                                                     >  1:252625/1‑131 (MQ=255)                                                                    cTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCaa                                                                     <  2:252625/131‑1 (MQ=255)                                                                    cTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCaa                                                                     >  1:416772/1‑131 (MQ=255)                                                                    cTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCaa                                                                     <  2:582142/131‑1 (MQ=255)                                                                    cTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGc                                                            >  2:592609/1‑140 (MQ=255)                                                                     tGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTCATCAAAGATGATGCa                                                           <  2:693545/140‑1 (MQ=255)                                                                       aTAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACt                                                         >  2:76499/1‑140 (MQ=255)                                                                          aCAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTAt                                                      >  1:526226/1‑140 (MQ=255)                                                                              gATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCAc                                                          >  2:486806/1‑132 (MQ=255)                                                                              gATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCAc                                                          <  1:486806/132‑1 (MQ=255)                                                                              gATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGAc                                                  >  1:155961/1‑140 (MQ=255)                                                                              gATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGAc                                                  >  1:814757/1‑140 (MQ=255)                                                                                   aaTCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGccc                                             >  1:807836/1‑140 (MQ=255)                                                                                      ccGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGGCACGCGGCGTATTACGGGGTTTGGCTTTGGTATCGAATTTGCGGCAAATATCGACaaaccaag                                          >  2:689661/1‑133 (MQ=255)                                                                                        gATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGt                                        >  1:54476/1‑140 (MQ=255)                                                                                        gATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGt                                        >  2:904923/1‑140 (MQ=255)                                                                                             ccAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGTGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGTTaaaa                                   >  1:861680/1‑140 (MQ=255)                                                                                               aaaaaTTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGa                                           <  2:950018/130‑1 (MQ=255)                                                                                               aaaaaTTATGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGa                                           >  1:950018/1‑130 (MQ=255)                                                                                                      aTGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATcccc                          >  2:73210/1‑140 (MQ=255)                                                                                                       tGCGGCGCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCg                         >  2:757305/1‑140 (MQ=255)                                                                                                        gcggcgCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCgg                        <  1:73210/140‑1 (MQ=255)                                                                                                         cggcgCTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGc                       <  2:526226/140‑1 (MQ=255)                                                                                                            cgcTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTg                    >  2:567064/1‑140 (MQ=255)                                                                                                             gcTGGCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGc                   >  2:948475/1‑140 (MQ=255)                                                                                                                 gCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTc                 <  2:747809/138‑1 (MQ=255)                                                                                                                 gCACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTc                 >  1:747809/1‑138 (MQ=255)                                                                                                                  cACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGc              <  1:948475/140‑1 (MQ=255)                                                                                                                  cACGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGc              <  1:960911/140‑1 (MQ=255)                                                                                                                   aCGCTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCg             <  2:149583/140‑1 (MQ=255)                                                                                                                      cTCGGTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTgg          >  2:2302/1‑140 (MQ=255)                                                                                                                         ggTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTAc       <  2:54476/140‑1 (MQ=255)                                                                                                                          gTGATAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCGCTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACg      <  1:689661/140‑1 (MQ=255)                                                                                                                              tAGCGACACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGt  <  1:806616/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                       TTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGGCACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCACTGTATGACGCGGTTGCGCTTTGTTATCAAAGATGATGCACTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGCTCGGCGTGGTACGTAGT  >  NC_000913/2839436‑2839700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑