Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,862,411	T→C	L26P (CTA→CCA)	ygbK →	putative 3‑oxo‑tetronate kinase YgbK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,862,411	0	T	C	100.0%	253.3 / NA	71	L26P (CTA→CCA)	ygbK	putative 3‑oxo‑tetronate kinase YgbK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/34);  total (37/34)

AGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGAAAACGCGCTCCTGTCCAGTGGTTGAAGCCACACAGCAATCGCTGGCGGCTCTGAGCTGGT  >  NC_000913/2862275‑2862545                                                                                                                                         |                                                                                                                                       agaagaCGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCAcc                                                                                                                                     <  1:516339/140‑1 (MQ=255) agaagaCGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCAcc                                                                                                                                     <  1:88878/140‑1 (MQ=255) agaagaCGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCAcc                                                                                                                                     >  1:372018/1‑140 (MQ=255) agaagaCGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCAcc                                                                                                                                     >  1:333961/1‑140 (MQ=255)      aCGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACgg                                                                                                                                <  1:147555/140‑1 (MQ=255)       cGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGt                                                                                                                               >  1:104082/1‑140 (MQ=255)          tAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACa                                                                                                                            >  2:787085/1‑140 (MQ=255)          tAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACa                                                                                                                            >  2:65514/1‑140 (MQ=255)           aGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTa                                                                                                                              >  1:496541/1‑137 (MQ=255)           aGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTa                                                                                                                              <  2:496541/137‑1 (MQ=255)             cgcAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAAt                                                                                                                         >  1:672/1‑140 (MQ=255)             cgcAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAAt                                                                                                                         >  2:803339/1‑140 (MQ=255)                aGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTaa                                                                                                                      >  1:10309/1‑140 (MQ=255)                aGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTaa                                                                                                                      <  1:803339/140‑1 (MQ=255)                aGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTaa                                                                                                                      <  2:10309/140‑1 (MQ=255)                aGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTaa                                                                                                                      <  1:158425/140‑1 (MQ=255)                 gTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAAc                                                                                                                     <  1:228585/140‑1 (MQ=255)                  ttATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCaa                                                                                                                                   <  2:525304/125‑1 (MQ=255)                  ttATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCaa                                                                                                                                   >  1:525304/1‑125 (MQ=255)                    aTCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGgt                                                                                                                  >  1:9119/1‑140 (MQ=255)                            tttCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAAc                                                                                                                     <  2:361929/129‑1 (MQ=255)                            tttCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAAc                                                                                                                     >  1:361929/1‑129 (MQ=255)                              tctcTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAg                                                                                                        >  1:557759/1‑140 (MQ=255)                                    gCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTaaaa                                                                                                  >  1:808026/1‑140 (MQ=255)                                        cACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGcc                                                                                              <  2:672/140‑1 (MQ=255)                                              aCCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGc                                                                                        >  1:255256/1‑140 (MQ=255)                                               ccGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCa                                                                                       >  2:262219/1‑140 (MQ=255)                                                 gggAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAAt                                                                                     <  1:787085/140‑1 (MQ=255)                                                 gggAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAAt                                                                                     <  2:9119/140‑1 (MQ=255)                                                  ggAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATc                                                                                    >  2:951289/1‑140 (MQ=255)                                                  ggAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATc                                                                                    >  2:414600/1‑140 (MQ=255)                                                    aGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGa                                                                                  <  1:671283/140‑1 (MQ=255)                                                     gCGAAGTCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGAc                                                                                 >  2:681940/1‑140 (MQ=255)                                                     gCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGAc                                                                                 >  2:498643/1‑140 (MQ=255)                                                      cGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACg                                                                                <  2:808026/140‑1 (MQ=255)                                                      cGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCCCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACg                                                                                <  1:535003/140‑1 (MQ=255)                                                      cGAAATCATGATCAAGAGTGGCGTTATCGCCGATGATTTTGCCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACg                                                                                <  1:65514/140‑1 (MQ=255)                                                       gAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGc                                                                               >  2:573326/1‑140 (MQ=255)                                                        aaaTCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCa                                                                              <  2:333961/140‑1 (MQ=255)                                                         aaTCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCAc                                                                             >  1:631568/1‑140 (MQ=255)                                                                aTCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGAt                                                                      <  1:681940/140‑1 (MQ=255)                                                                aTCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGAt                                                                      <  2:104082/140‑1 (MQ=255)                                                                   aaGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAg                                                                   >  2:609373/1‑140 (MQ=255)                                                                      aTTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCt                                                                >  1:287132/1‑140 (MQ=255)                                                                        tGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGa                                                              >  2:285632/1‑140 (MQ=255)                                                                             ttATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGAAAAcg                                                         >  1:645803/1‑140 (MQ=255)                                                                             ttATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGAAAAcg                                                         <  1:285632/140‑1 (MQ=255)                                                                             ttATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGAAAAcg                                                         <  2:16768/140‑1 (MQ=255)                                                                             ttATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGAAAAcg                                                         <  1:498643/140‑1 (MQ=255)                                                                             ttATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGAAAAcg                                                         <  2:901267/140‑1 (MQ=255)                                                                               aTCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGAAAAcgcg                                                       >  2:751920/1‑140 (MQ=255)                                                                               aTCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGAAAAcgcg                                                       >  1:296924/1‑140 (MQ=255)                                                                               aTCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGAAAAcgcg                                                       <  1:197298/140‑1 (MQ=255)                                                                               aTCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGAAAAcgcg                                                       >  2:407930/1‑140 (MQ=255)                                                                               aTCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGAAAAcgcg                                                       >  1:754680/1‑140 (MQ=255)                                                                               aTCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGAAAAcgcg                                                       <  1:407930/140‑1 (MQ=255)                                                                               aTCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGAAAAcg                                                         >  1:248462/1‑138 (MQ=255)                                                                               aTCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGAAAAcg                                                         <  2:248462/138‑1 (MQ=255)                                                                                tCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGAt                                                                      <  1:596971/124‑1 (MQ=255)                                                                                tCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGAt                                                                      >  2:596971/1‑124 (MQ=255)                                                                                tCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGAt                                                                      <  1:405989/124‑1 (MQ=255)                                                                                tCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGAt                                                                      >  2:405989/1‑124 (MQ=255)                                                                                    cGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGAAAACGCGCTCCt                                                  >  1:736144/1‑140 (MQ=255)                                                                                       tgatTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGAAAACGCGCTCCTGTc                                               <  1:414600/140‑1 (MQ=255)                                                                                              aCCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGAAAACGCGCTCCTGTCCAGTGGt                                        <  2:645803/140‑1 (MQ=255)                                                                                                   cgcgACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGAAAACGCGCTCCTGTCCAGTGGTTGAAg                                   >  2:239976/1‑140 (MQ=255)                                                                                                   cgcgACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGAAAACGCGCTCCTGTCCAGTGGTTGAAg                                   <  2:631568/140‑1 (MQ=255)                                                                                                   cgcgACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGAAAACGCGCTCCTGTCCAGTGGTTGAAg                                   >  1:291185/1‑140 (MQ=255)                                                                                                             atCGCCAGTTTTCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGAAAACGCGCTCCTGTCCAGTGGTTGAAGCCACACAGCa                         >  1:308522/1‑140 (MQ=255)                                                                                                                     ttttCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGAAAACGCGCTCCTGTCCAGTGGTTGAAGCCACACAGCAATCGCTgg                 <  1:239976/140‑1 (MQ=255)                                                                                                                     ttttCTGGTGGAAAACGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGAAAACGCGCTCCTGTCCAGTGGTTGAAGCCACACAGCAATCGCTgg                 <  2:372018/140‑1 (MQ=255)                                                                                                                                    cGGTCCACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGAAAACGCGCTCCTGTCCAGTGGTTGAAGCCACACAGCAATCGCTGGCGGCTCTGAGCTGGt  >  1:77991/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       AGAAGACGATAGCGCAGTTATCAAGATTTTCTCTGGCATCACTCTACCGGGAGCGAAATCATGATCAAGATTGGCGTTATCGCCGATGATTTTACCGGCGCGACGGATATCGCCAGTTTTCTGGTGGAAAACGGTCTACCAACGGTACAAATTAACGGTGTTCCAACAGGTAAAATGCCGGAAGCAATCGACGCACTGGTGATCAGCCTGAAAACGCGCTCCTGTCCAGTGGTTGAAGCCACACAGCAATCGCTGGCGGCTCTGAGCTGGT  >  NC_000913/2862275‑2862545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑