Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,865,178	A→G	V26V (GTA→GTG)	ygbN →	putative transporter YgbN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,865,178	0	A	G	100.0%	176.9 / NA	50	V26V (GTA→GTG)	ygbN	putative transporter YgbN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/30);  total (20/30)

TCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTACAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGCTTGGTTCCGTCACTATTATTA  >  NC_000913/2865047‑2865305                                                                                                                                    |                                                                                                                                 tCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCAtt                                                                                                                          >  1:111936/1‑140 (MQ=255) tCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTACACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCAtt                                                                                                                          >  2:917045/1‑140 (MQ=255)  ccccccATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATGATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTc                                                                                                                         >  2:898808/1‑140 (MQ=255)   cccccATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCg                                                                                                                        <  2:756684/140‑1 (MQ=255)    ccccATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGt                                                                                                                       >  1:16214/1‑140 (MQ=255)    ccccATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGt                                                                                                                       >  1:665949/1‑140 (MQ=255)    ccccATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGt                                                                                                                       >  2:462424/1‑140 (MQ=255)       cATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGttgc                                                                                                                    >  1:428323/1‑140 (MQ=255)       cATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGttgc                                                                                                                    >  1:827012/1‑140 (MQ=255)               gATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGctcc                                                                                                            <  2:477875/140‑1 (MQ=255)               gATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCAGTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGctcc                                                                                                            >  1:839657/1‑140 (MQ=255)                aTGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGctcct                                                                                                           <  1:462424/140‑1 (MQ=255)                  ggggTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCg                                                                                                         >  2:310403/1‑140 (MQ=255)                  ggggTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCg                                                                                                         <  1:321548/140‑1 (MQ=255)                   gggTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTTCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGt                                                                                                        >  2:690259/1‑140 (MQ=255)                   gggTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGt                                                                                                        >  2:249280/1‑140 (MQ=255)                   gggTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGt                                                                                                        >  2:104296/1‑140 (MQ=255)                   gggTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGCGCAACCATTCGTTGCTTTGCTCCTCGt                                                                                                        >  2:110220/1‑140 (MQ=255)                     gTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCa                                                                                                      <  1:792787/140‑1 (MQ=255)                     gTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCa                                                                                                      <  2:947843/140‑1 (MQ=255)                       aGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGc                                                                                                    <  1:898808/140‑1 (MQ=255)                               tataACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTCCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGt                                                                                            <  2:665949/140‑1 (MQ=255)                               tataACTTTCAGACAGGGTTTCCCATGTACACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGt                                                                                            <  1:917045/140‑1 (MQ=255)                                   aCTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCa                                                                                        >  2:4282/1‑140 (MQ=255)                                       tcagtcagGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTg                                                                                    >  2:686097/1‑140 (MQ=255)                                        cAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGc                                                                                   <  1:385706/140‑1 (MQ=255)                                         aGACAGGGTTTCCCATGTTCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCg                                                                                  <  1:690259/140‑1 (MQ=255)                                           aCAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGc                                                                                <  1:278420/140‑1 (MQ=255)                                           aCAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGc                                                                                <  1:310403/140‑1 (MQ=255)                                           aCAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGc                                                                                <  2:839657/140‑1 (MQ=255)                                                   tCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATAc                                                                        >  1:45541/1‑140 (MQ=255)                                                             acaATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGa                                                             <  2:16214/140‑1 (MQ=255)                                                                     aTTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTa                                                     <  2:675729/140‑1 (MQ=255)                                                                      ttGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGGGGAGgggggga                                                    >  2:824190/1‑137 (MQ=255)                                                                       tGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTaaa                                                   <  1:686097/140‑1 (MQ=255)                                                                            tGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGCGGGGGGAAGGGGGGAAAgggg                                               >  2:539871/1‑137 (MQ=255)                                                                                  gCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGc                                        <  2:428323/140‑1 (MQ=255)                                                                                   cGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGcc                                       <  1:107933/140‑1 (MQ=255)                                                                                       aGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGGGGAGGGGGGGAAAAGAAGATTGCCgggg                                   >  2:107933/1‑139 (MQ=255)                                                                                             cGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCg                             <  1:539871/140‑1 (MQ=255)                                                                                             cGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCg                             <  1:824190/140‑1 (MQ=255)                                                                                               tAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGgt                           <  2:646600/140‑1 (MQ=255)                                                                                               tAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGgt                           <  1:706132/140‑1 (MQ=255)                                                                                                   cATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGc                       <  2:827012/140‑1 (MQ=255)                                                                                                    aTGCTGTTGCTGCTGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGCt                      <  1:468143/140‑1 (MQ=255)                                                                                                            gctgctGGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGCTTGGTTCCg              <  1:36688/140‑1 (MQ=255)                                                                                                              tgctgGTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGCTTGGTTCCGTc            <  1:4282/140‑1 (MQ=255)                                                                                                                  ggTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGCTTGGTTCCGTCACta        <  1:884671/140‑1 (MQ=255)                                                                                                                   gTCATCAAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGCTTGGTTCCGTCACtat       <  1:826875/140‑1 (MQ=255)                                                                                                                        caAGGCAAAGGTGCAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGCTTGGTTCCGTCACtattatta  <  1:104296/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                 TCCCCCCATCTACGGATGGGGTAGGTTAACTATAACTTTCAGACAGGGTTTCCCATGTCCACAATTACATTGTTATGCATTGCGTTAGCTGGCGTAATCATGCTGTTGCTGCTGGTCATCAAGGCAAAGGTACAACCATTCGTTGCTTTGCTCCTCGTCAGCCTGTTAGTCGCACTTGCGGCAGGTATACCGGC‑GGGCGAAGTGGGTAAAGTGATGATCGCCGGGATGGGCGGTGTGCTTGGTTCCGTCACTATTATTA  >  NC_000913/2865047‑2865305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑