Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,970,592	T→C	V58A (GTT→GCT)	ygdQ →	UPF0053 inner membrane protein YgdQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,970,592	0	T	C	100.0%	190.5 / NA	53	V58A (GTT→GCT)	ygdQ	UPF0053 inner membrane protein YgdQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/26);  total (27/26)

TTCTTGGGATCGACAATATTATTTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGTTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTGGGTGGCTTGTTCCTTATCTGGAAA  >  NC_000913/2970490‑2970725                                                                                                       |                                                                                                                                      ttCTTGGGATCGACAATATTATTTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGtt                                                                                                  <  2:507695/140‑1 (MQ=255) ttCTTGGGATCGACAATATTATTTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGtt                                                                                                  >  2:179378/1‑140 (MQ=255) ttCTTGGGATCGACAATATTATTTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGtt                                                                                                  >  2:149900/1‑140 (MQ=255)            gACAATATTATTTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGAc                                                                                       >  2:885534/1‑140 (MQ=255)                  attattTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATcc                                                                                 <  1:885534/140‑1 (MQ=255)                   ttattTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCg                                                                                <  1:526381/140‑1 (MQ=255)                   ttattTTCCTTTCTCCGGTGGTGGAATAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGTGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCGTGACAAATCCg                                                                                <  2:259122/140‑1 (MQ=255)                     attTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCt                                                                              <  1:899847/140‑1 (MQ=255)                        ttCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCtttt                                                                           >  2:927529/1‑140 (MQ=255)                           cTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTAc                                                                        <  1:149900/140‑1 (MQ=255)                             ttCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACaa                                                                      >  2:597851/1‑140 (MQ=255)                              tctcTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAat                                                                     >  2:824600/1‑140 (MQ=255)                              tctcTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAat                                                                     >  2:842405/1‑140 (MQ=255)                                 ctGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATAtt                                                                  <  1:235769/140‑1 (MQ=255)                                 ctGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATAtt                                                                  <  1:597851/140‑1 (MQ=255)                                    gtggtggCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATtcag                                                               >  2:847540/1‑140 (MQ=255)                                     tggtggCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATAtt                                                                  <  1:54824/136‑1 (MQ=255)                                     tggtggCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATAtt                                                                  >  2:54824/1‑136 (MQ=255)                                     tggtggCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATtcagt                                                              <  1:377879/140‑1 (MQ=255)                                     tggtggCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATtcagt                                                              <  1:826765/140‑1 (MQ=255)                                       gtggCAAAGCTTCCCACAGCACTACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATtcagtca                                                            >  1:480752/1‑140 (MQ=255)                                       gtggCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATtcagtca                                                            <  2:8672/140‑1 (MQ=255)                                       gtggCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATtcagtca                                                            <  1:847540/140‑1 (MQ=255)                                       gtggCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATtcagt                                                              <  1:439617/138‑1 (MQ=255)                                       gtggCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATtcagt                                                              >  2:439617/1‑138 (MQ=255)                                         ggCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAgg                                                          >  1:170025/1‑140 (MQ=255)                                          gCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGa                                                         >  1:137708/1‑140 (MQ=255)                                               gCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATtcagt                                                              <  2:765548/130‑1 (MQ=255)                                               gCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATACGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATtcagt                                                              >  1:765548/1‑130 (MQ=255)                                                cTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTc                                                   <  1:927529/140‑1 (MQ=255)                                                    ccACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGcc                                               >  1:490381/1‑140 (MQ=255)                                                    ccACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGcc                                               <  1:842405/140‑1 (MQ=255)                                                         gCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGTCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGtga                                          >  2:638642/1‑140 (MQ=255)                                                          cacaACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTCTTACAATATTCAGTCAGGAAATTTCTGCCCGtgat                                         >  1:662913/1‑140 (MQ=255)                                                            caACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGtgattt                                       <  1:824600/140‑1 (MQ=255)                                                             aaCGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCAT‑AATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGtgattt                                       >  1:545951/1‑138 (MQ=255)                                                             aaCGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCAT‑AATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGtgattt                                       <  2:545951/138‑1 (MQ=255)                                                               cGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGtgatttgat                                    >  2:69430/1‑140 (MQ=255)                                                                gTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGTCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGtgatttgatt                                   <  1:638642/140‑1 (MQ=255)                                                                  gCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCt                                 >  1:239663/1‑140 (MQ=255)                                                                  gCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCt                                 >  2:121688/1‑140 (MQ=255)                                                                    tCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGc                               >  2:492959/1‑140 (MQ=255)                                                                          gcgcCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTgg                         <  2:239663/140‑1 (MQ=255)                                                                           cgcCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTggg                        >  2:62858/1‑140 (MQ=255)                                                                           cgcCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTggg                        >  1:142402/1‑140 (MQ=255)                                                                           cgcCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTggg                        >  2:11459/1‑140 (MQ=255)                                                                           cgcCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTggg                        >  2:191717/1‑140 (MQ=255)                                                                                tCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTGGGTGGCt                   <  1:756372/140‑1 (MQ=255)                                                                                tCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTGGGTGGCt                   <  1:492959/140‑1 (MQ=255)                                                                                     ggTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTGGGTGGCTTGTTc              <  1:368349/140‑1 (MQ=255)                                                                                      gTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTGGGTGGCTTGTTcc             <  2:142402/140‑1 (MQ=255)                                                                                      gTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCAGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTCTTACAATATTCAGTCAGCAAATTTCTGCCCGTGATTTGATTCTGCTTCTGGGTGGCTTGTTcc             <  2:662913/140‑1 (MQ=255)                                                                                          gCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTGGGTGGCTTGTTCCTTAt         >  1:629133/1‑140 (MQ=255)                                                                                                 ccATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTGGGTGGCTTGTTCCTTATCTGGaaa  <  1:179378/140‑1 (MQ=255)                                                                                                       |                                                                                                                                      TTCTTGGGATCGACAATATTATTTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGTTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTGGGTGGCTTGTTCCTTATCTGGAAA  >  NC_000913/2970490‑2970725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑