Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	342,291	T→C	R389R (CGT→CGC)	yahJ →	putative deaminase with metallo‑dependent hydrolase domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	342,291	0	T	C	100.0%	199.0 / NA	57	R389R (CGT→CGC)	yahJ	putative deaminase with metallo‑dependent hydrolase domain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/21);  total (36/21)

CATATGCCGCTCAAACAGTTGCACGACAAAGGCGTAAAAGTGATGACTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACGCCT  >  NC_000913/342154‑342418                                                                                                                                          |                                                                                                                                cATATGCCGCTCAAACAGTTGCACGACAAAGGCGTAAAAGTGATGACTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGccc                                                                                                                               >  1:151913/1‑140 (MQ=255)   tatGCCGCTCAAACAGTTGCACGACAAAGGCGTAAAAGTGATGACTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTa                                                                                                                             >  1:690184/1‑140 (MQ=255)          cTCAAACAGTTGCACGACAAAGGCGTAAAAGTGATGACTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACa                                                                                                                      >  1:561952/1‑140 (MQ=255)           tCAAACAGTTGCACGACAAAGGCGTAAAAGTGATGACTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTaa                                                                                                                            >  2:314393/1‑133 (MQ=255)           tCAAACAGTTGCACGACAAAGGCGTAAAAGTGATGACTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTaa                                                                                                                            <  1:314393/133‑1 (MQ=255)           tCAAACAGTTGCACGACAAAGGCGTAAAAGTGATGACTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAg                                                                                                                     <  2:684407/140‑1 (MQ=255)           tCAAACAGTTGCACGACAAAGGCGTAAAAGTGATGACTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAg                                                                                                                     >  1:72091/1‑140 (MQ=255)           tCAAACAGTTGCACGACAAAGGCGTAAAAGTGATGACTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAg                                                                                                                     >  1:684407/1‑140 (MQ=255)           tCAAACAGTTGCACGACAAAGGCGTAAAAGTGATGACTGGCACTGACAGCGTTATCGACCACTGGGCGACTTATGGTCCGCGCGAAATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAg                                                                                                                     >  2:636736/1‑140 (MQ=255)             aaaCAGTTGCACGACAAAGGCGTAAAAGTGATGACTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGaa                                                                                                                   >  2:245797/1‑140 (MQ=255)              aaCAGTTGCACGACAAAGGCGTAAAAGTGATGACTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAAc                                                                                                                  <  1:245797/140‑1 (MQ=255)                  gTTGCACGACAAAGGCGTAAAAGTGATGACTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACctct                                                                                                              <  2:690184/140‑1 (MQ=255)                     gCACGACAAAGGCGTAAAAGTGATGACTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTccc                                                                                                           >  2:420972/1‑140 (MQ=255)                     gCACGACAAAGGCGTAAAAGTGATGACTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTccc                                                                                                           <  2:72091/140‑1 (MQ=255)                           cAAAGGCGTAAAAGTGATGACTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCcgctcgc                                                                                                     >  1:588568/1‑140 (MQ=255)                           cAAAGGCGTAAAAGTGATGACTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCcgctcgc                                                                                                     <  1:420972/140‑1 (MQ=255)                              aGGCGTAAAAGTGATGACTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGt                                                                                                  >  2:790623/1‑140 (MQ=255)                                        gTGATGACTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCAc                                                                                        <  2:588568/140‑1 (MQ=255)                                          gatgaCTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTg                                                                                      >  1:832003/1‑140 (MQ=255)                                          gatgaCTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTg                                                                                      >  1:722393/1‑140 (MQ=255)                                           atgaCTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTgg                                                                                     >  2:880297/1‑140 (MQ=255)                                           atgaCTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTgg                                                                                     <  1:790623/140‑1 (MQ=255)                                           atgaCTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTAAGcacttgg                                                                                     >  1:508883/1‑134 (MQ=255)                                             gaCTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCg                                                                                   <  1:880297/140‑1 (MQ=255)                                                            cGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTgg                                                                                     <  1:658147/123‑1 (MQ=255)                                                            cGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTgg                                                                                     >  2:658147/1‑123 (MQ=255)                                                            cGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCtg                                                                     >  2:645322/1‑139 (MQ=255)                                                            cGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCtg                                                                     <  1:645322/139‑1 (MQ=255)                                                             gTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCtgaa                                                                   <  2:722393/140‑1 (MQ=255)                                                              ttATCGACCACTGGTCGCCTTATGGTCTTGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCtgaat                                                                  >  1:208271/1‑140 (MQ=255)                                                              ttATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCtgaat                                                                  >  1:376977/1‑140 (MQ=255)                                                                      cACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAgg                                                          >  2:669656/1‑140 (MQ=255)                                                                         tGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGa                                                       >  2:716789/1‑140 (MQ=255)                                                                           gTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGc                                                     >  2:400044/1‑140 (MQ=255)                                                                               ccTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGa                                                       <  1:78716/134‑1 (MQ=255)                                                                               ccTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGa                                                       >  2:78716/1‑134 (MQ=255)                                                                                       tCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCaa                                         <  2:561952/140‑1 (MQ=255)                                                                                         tGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCaaaa                                       >  1:836116/1‑140 (MQ=255)                                                                                          gggCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAg                                      >  2:693804/1‑140 (MQ=255)                                                                                           ggCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAgc                                     >  2:393385/1‑140 (MQ=255)                                                                                            gCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAgcg                                    >  1:224750/1‑140 (MQ=255)                                                                                                cATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAgg                                >  2:884468/1‑140 (MQ=255)                                                                                                 aTGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGa                               >  1:742445/1‑140 (MQ=255)                                                                                                     tGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGAc                           >  1:128496/1‑140 (MQ=255)                                                                                                       gAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGc                         <  1:884468/140‑1 (MQ=255)                                                                                                          aaaGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAgcgc                                   <  2:20056/127‑1 (MQ=255)                                                                                                          aaaGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAgcgc                                   >  1:20056/1‑127 (MQ=255)                                                                                                          aaaGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAg                      >  1:560809/1‑140 (MQ=255)                                                                                                           aaGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGc                     <  2:832003/140‑1 (MQ=255)                                                                                                            aGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCt                    <  2:560809/140‑1 (MQ=255)                                                                                                            aGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCt                    >  2:677928/1‑140 (MQ=255)                                                                                                            aGCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCt                    <  1:400044/140‑1 (MQ=255)                                                                                                             gCCAATCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCtt                   >  2:58096/1‑140 (MQ=255)                                                                                                                 aTCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCTTtgtg               >  2:555315/1‑140 (MQ=255)                                                                                                                 aTCTGTACGCGCAGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCTTtgtg               <  1:693804/140‑1 (MQ=255)                                                                                                                             aGCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACGcc   <  2:224750/140‑1 (MQ=255)                                                                                                                              gCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGATCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACGcct  >  2:672777/1‑140 (MQ=255)                                                                                                                              gCTCTATATTCGCCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACGcct  >  1:875837/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                CATATGCCGCTCAAACAGTTGCACGACAAAGGCGTAAAAGTGATGACTGGCACTGACAGCGTTATCGACCACTGGTCGCCTTATGGTCTGGGCGACATGCTGGAAAAAGCCAATCTGTACGCGCAGCTCTATATTCGTCCTAACGAACAGAACCTCTCCCGCTCGCTGTTTTTAGCCACTGGCGATGTATTGCCGCTGAATGAAAAAGGCGAGCGTGTATGGCCAAAAGCGCAGGATGACGCCAGCTTTGTGCTGGTGGACGCCT  >  NC_000913/342154‑342418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑