Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,977,063	T→C	C155C (TGT→TGC)	galR →	DNA‑binding transcriptional dual regulator GalR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,977,063	0	T	C	100.0%	196.3 / NA	55	C155C (TGT→TGC)	galR	DNA‑binding transcriptional dual regulator GalR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/30);  total (25/30)

AACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGA  >  NC_000913/2976927‑2977197                                                                                                                                         |                                                                                                                                       aaCTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCAtt                                                                                                                                     >  2:955419/1‑140 (MQ=255) aaCTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCAtt                                                                                                                                     >  1:857116/1‑140 (MQ=255) aaCTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCAtt                                                                                                                                     >  2:807442/1‑140 (MQ=255)  aCTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTg                                                                                                                                    <  1:25266/140‑1 (MQ=255)  aCTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTg                                                                                                                                    <  2:583144/140‑1 (MQ=255)  aCTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTg                                                                                                                                    >  1:40740/1‑140 (MQ=255)   cTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGc                                                                                                                                   <  2:782135/140‑1 (MQ=255)    tGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGct                                                                                                                                  <  2:245697/140‑1 (MQ=255)      aTCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGctct                                                                                                                                >  2:364181/1‑140 (MQ=255)        ccGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTgg                                                                                                                              >  2:229176/1‑140 (MQ=255)          gctaTCGCTGTGCTGCGTTGGTGGGCGATGCCAAAATGATCCCGGATGTTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCCGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGAc                                                                                                                            <  2:96065/137‑1 (MQ=255)             aTCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGAt                                                                                                                         >  2:329368/1‑140 (MQ=255)              tCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATc                                                                                                                        <  2:540916/140‑1 (MQ=255)                        gcGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTCGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGc                                                                                                              <  2:857116/140‑1 (MQ=255)                          gTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCct                                                                                                            <  2:40740/140‑1 (MQ=255)                              gtcgtcCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCctggct                                                                                                        >  1:706232/1‑140 (MQ=255)                                   ccATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCaa                                                                                                   >  1:861588/1‑140 (MQ=255)                                   ccATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCaa                                                                                                   >  1:496160/1‑140 (MQ=255)                                     aTGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAAcg                                                                                                 <  2:379596/140‑1 (MQ=255)                                       gCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAAcgcg                                                                                               <  2:496160/140‑1 (MQ=255)                                       gCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAAcgcg                                                                                               <  1:955419/140‑1 (MQ=255)                                            aaTGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCAtt                                                                                          >  1:746773/1‑140 (MQ=255)                                              tGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTa                                                                                        <  1:807442/140‑1 (MQ=255)                                                      gATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCa                                                                                <  2:98728/140‑1 (MQ=255)                                                             aTTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCAt                                                                         >  1:270933/1‑140 (MQ=255)                                                              tttAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATa                                                                        <  2:359900/140‑1 (MQ=255)                                                                tAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATAcc                                                                      <  1:364181/140‑1 (MQ=255)                                                                  gCCTCATTAATGAAACAAATGCTCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCg                                                                    <  2:706232/140‑1 (MQ=255)                                                                  gCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCg                                                                    <  1:329368/140‑1 (MQ=255)                                                                       aTTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTg                                                               >  2:379162/1‑140 (MQ=255)                                                                         tAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGt                                                             <  2:604792/140‑1 (MQ=255)                                                                             gAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATc                                                         >  1:455689/1‑140 (MQ=255)                                                                                aCAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATc                                                         <  2:953392/137‑1 (MQ=255)                                                                                aCAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATc                                                         >  1:953392/1‑137 (MQ=255)                                                                                 cAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCg                                                                    <  2:906115/125‑1 (MQ=255)                                                                                 cAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCg                                                                    >  1:906115/1‑125 (MQ=255)                                                                                 cAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCtgtg                                                     >  2:327065/1‑140 (MQ=255)                                                                                 cAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCtgtg                                                     >  2:372782/1‑140 (MQ=255)                                                                                        ccGGTATGGTGCTGATTAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAAc                                              <  2:861588/140‑1 (MQ=255)                                                                                                    tGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAAc                                              <  2:245429/128‑1 (MQ=255)                                                                                                    tGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAAc                                              >  1:245429/1‑128 (MQ=255)                                                                                                     gATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTg                                 <  2:455689/140‑1 (MQ=255)                                                                                                      aTCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGa                                >  1:213254/1‑140 (MQ=255)                                                                                                      aTCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGa                                >  1:177451/1‑140 (MQ=255)                                                                                                                tCCTGCCTGGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGAt                      >  2:363297/1‑140 (MQ=255)                                                                                                                     cctgGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCt                 >  1:949534/1‑140 (MQ=255)                                                                                                                     cctgGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCt                 >  1:754084/1‑140 (MQ=255)                                                                                                                       tgGCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGc               <  2:746773/140‑1 (MQ=255)                                                                                                                        ggCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCa              >  2:665559/1‑140 (MQ=255)                                                                                                                         gCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTTAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCaa             <  2:670625/140‑1 (MQ=255)                                                                                                                         gCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCaa             <  1:612655/140‑1 (MQ=255)                                                                                                                         gCTTTGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCaa             <  1:229176/140‑1 (MQ=255)                                                                                                                            ttGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAggg          <  1:363297/140‑1 (MQ=255)                                                                                                                             tGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGt         <  1:327065/140‑1 (MQ=255)                                                                                                                             tGAAAACCGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGt         <  1:372782/140‑1 (MQ=255)                                                                                                                                    cGTTGCATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGa  <  1:665559/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       AACTGATCCGCCATCGCTGTGCTGCGTTGGTCGTCCATGCCAAAATGATCCCGGATGCTGATTTAGCCTCATTAATGAAACAAATGCCCGGTATGGTGCTGATCAACCGTATCCTGCCTGGCTTTGAAAACCGTTGTATTGCTCTGGACGATCGTTACGGTGCCTGGCTGGCAACGCGTCATTTAATTCAGCAAGGTCATACCCGCATTGGTTATCTGTGCTCTAACCACTCTATTTCTGACGCCGAAGATCGTCTGCAAGGGTATTACGA  >  NC_000913/2976927‑2977197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑