Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,984,896	G→A	P233S (CCT→TCT)	yqeF ←	putative acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,984,896	0	G	A	100.0%	150.5 / NA	43	P233S (CCT→TCT)	yqeF	putative acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/18);  total (25/18)

GCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGC  >  NC_000913/2984765‑2985028                                                                                                                                    |                                                                                                                                     gcgAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAAc                                                                                                                              >  2:243661/1‑140 (MQ=255)               gCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCt                                                                                                                <  2:898705/140‑1 (MQ=255)               gCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCt                                                                                                                >  1:792756/1‑140 (MQ=255)                 aCGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTgc                                                                                                              <  1:243661/140‑1 (MQ=255)                     gTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTg                                                                                                          >  1:161500/1‑140 (MQ=255)                          ttCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTc                                                                                                     >  1:702218/1‑140 (MQ=255)                                           ggCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCAt                                                                                    <  2:161500/140‑1 (MQ=255)                                              ttCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCgg                                                                                 <  1:863696/140‑1 (MQ=255)                                                cGCTCATCATCATTACCGCAGCTGCGCGATCGTTTATGGATGATGCATTACCCGCTGTCTCAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTa                                                                               <  2:903912/140‑1 (MQ=255)                                                        atcatTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCaacaac                                                                       <  2:702218/140‑1 (MQ=255)                                                         tcatTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACTGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAAcaacc                                                                      <  1:816932/140‑1 (MQ=255)                                                             tACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACg                                                                  >  2:806529/1‑140 (MQ=255)                                                                cGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCt                                                               <  1:97088/140‑1 (MQ=255)                                                                 gCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTg                                                              >  1:766047/1‑140 (MQ=255)                                                                  cAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTg                                                              <  2:166878/139‑1 (MQ=255)                                                                  cAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTg                                                              >  1:166878/1‑139 (MQ=255)                                                                    gCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAAcaacca                                                                     <  1:133517/130‑1 (MQ=255)                                                                    gCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAAcaacca                                                                     >  2:133517/1‑130 (MQ=255)                                                                    gCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCt                                                               >  1:72862/1‑136 (MQ=255)                                                                    gCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCt                                                               <  2:72862/136‑1 (MQ=255)                                                                    gCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGccc                                                           >  2:625882/1‑140 (MQ=255)                                                                     cTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCg                                                          >  2:825576/1‑140 (MQ=255)                                                                       gcgcCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGtt                                                        >  1:170186/1‑140 (MQ=255)                                                                        cgcCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTa                                                       >  2:227518/1‑140 (MQ=255)                                                                         gcCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTAc                                                      >  1:763130/1‑140 (MQ=255)                                                                                tttATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGt                                               <  1:654300/140‑1 (MQ=255)                                                                                     ggATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTa                                          <  2:766047/140‑1 (MQ=255)                                                                                       atgatgCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTGCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTAcc                                        >  1:840424/1‑140 (MQ=255)                                                                                          atgCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCggg                                     >  1:376985/1‑140 (MQ=255)                                                                                           tgCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTc                                              >  1:522860/1‑130 (MQ=255)                                                                                           tgCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTc                                              <  2:522860/130‑1 (MQ=255)                                                                                                tACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGAtc                               >  2:897299/1‑140 (MQ=255)                                                                                                 aCCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGAtct                              >  1:234072/1‑140 (MQ=255)                                                                                                 aCCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGAtct                              <  2:840424/140‑1 (MQ=255)                                                                                                  cccGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGAtctc                             >  1:778564/1‑140 (MQ=255)                                                                                                  cccGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGAtct                              >  1:835351/1‑139 (MQ=255)                                                                                                  cccGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGAtct                              <  2:835351/139‑1 (MQ=255)                                                                                                     gCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATc                          >  1:947226/1‑140 (MQ=255)                                                                                                       tGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCtt                        >  2:318219/1‑140 (MQ=255)                                                                                                          cacaGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTaa                     <  1:318219/140‑1 (MQ=255)                                                                                                                  cGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCg             <  1:625882/140‑1 (MQ=255)                                                                                                                    agaCTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCACTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGc           >  1:228514/1‑140 (MQ=255)                                                                                                                             aaTGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATcgc  >  2:858001/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                     GCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGC  >  NC_000913/2984765‑2985028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑