Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,986,581	A→G	V245V (GTA→GTG)	yqeG →	putative transporter YqeG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,986,581	0	A	G	100.0%	148.4 / NA	42	V245V (GTA→GTG)	yqeG	putative transporter YqeG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/24);  total (18/24)

GGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCG  >  NC_000913/2986449‑2986711                                                                                                                                     |                                                                                                                                   ggTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCt                                                                                                                             >  2:424986/1‑140 (MQ=255) ggTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCt                                                                                                                             <  1:424986/140‑1 (MQ=255)             tAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCt                                                                                                                       >  2:876317/1‑134 (MQ=255)             tAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCt                                                                                                                       <  1:876317/134‑1 (MQ=255)                    aCGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCtt                                                                                                                      <  2:59011/128‑1 (MQ=255)                    aCGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCtt                                                                                                                      >  1:59011/1‑128 (MQ=255)                    aCGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCt                                                                                                          >  1:98519/1‑140 (MQ=255)                     cGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCtt                                                                                                         <  2:405308/140‑1 (MQ=255)                           ttattTCTTCGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTAtt                                                                                                   <  2:682232/140‑1 (MQ=255)                           ttattTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTAtt                                                                                                   <  1:613398/140‑1 (MQ=255)                             attTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTcc                                                                                                 <  2:98519/140‑1 (MQ=255)                              tttCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCa                                                                                                <  2:903689/140‑1 (MQ=255)                                  tACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCtt                                                                                            <  1:46083/140‑1 (MQ=255)                                  tACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCtt                                                                                            >  1:74394/1‑140 (MQ=255)                                    cGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCg                                                                                          >  1:329646/1‑140 (MQ=255)                                      tttGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCa                                                                                                <  2:513241/132‑1 (MQ=255)                                      tttGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCa                                                                                                >  1:513241/1‑132 (MQ=255)                                      tttGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCA‑CTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGtat                                                                                       >  1:609284/1‑140 (MQ=255)                                        tGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTc                                                                                                  <  1:873775/128‑1 (MQ=255)                                        tGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTc                                                                                                  >  2:873775/1‑128 (MQ=255)                                        tGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGtata                                                                                      >  1:354168/1‑140 (MQ=255)                                             tAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGaa                                                                                 <  2:209069/140‑1 (MQ=255)                                             tAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGaa                                                                                 <  2:354168/140‑1 (MQ=255)                                              agacagacGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCA‑CTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAgc                                                                               <  1:660228/140‑1 (MQ=255)                                               gacagacGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAgc                                                                               <  2:322469/140‑1 (MQ=255)                                               gacagacGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAgc                                                                               <  2:329646/140‑1 (MQ=255)                                                    acGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTa                                                                          >  1:563752/1‑140 (MQ=255)                                                                 ggCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGt                                                             <  2:265101/140‑1 (MQ=255)                                                                                gATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGgc                                              >  1:228577/1‑140 (MQ=255)                                                                                gATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGgc                                              <  2:641998/140‑1 (MQ=255)                                                                                      tGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTc                                        >  1:904727/1‑140 (MQ=255)                                                                                       gCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCt                                       <  2:228577/140‑1 (MQ=255)                                                                                        cAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTa                                      >  2:331954/1‑140 (MQ=255)                                                                                        cAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTa                                      >  2:940700/1‑140 (MQ=255)                                                                                          aaaaaaTTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTAtg                                    >  1:314904/1‑140 (MQ=255)                                                                                          aaaaaaTTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTAtg                                    >  1:277732/1‑140 (MQ=255)                                                                                                 tATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGa                             <  1:940700/140‑1 (MQ=255)                                                                                                       aGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGccc                       <  2:74394/140‑1 (MQ=255)                                                                                                           gCTTATCTCATCA‑CTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCa                  <  2:609284/140‑1 (MQ=255)                                                                                                                    atcatcTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTc          >  2:381292/1‑140 (MQ=255)                                                                                                                          tGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTc    <  2:337878/140‑1 (MQ=255)                                                                                                                          tGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTc    <  2:904727/140‑1 (MQ=255)                                                                                                                            cATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCg  >  1:909070/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                   GGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCG  >  NC_000913/2986449‑2986711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑