Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,011,789	T→C	V110A (GTT→GCT)	yqeA →	putative amino acid kinase YqeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,011,789	0	T	C	100.0%	200.3 / NA	59	V110A (GTT→GCT)	yqeA	putative amino acid kinase YqeA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/31);  total (28/31)

ACTGAAGCGCACTCGCCAATGCTGCCGATGTCTGTCTGTGTTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGTTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCTGGTCG  >  NC_000913/3011653‑3011921                                                                                                                                         |                                                                                                                                     aCTGAAGCGCACTCGCCAATGCTGCCGATGTCTGTCTGTGTTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTAc                                                                                                                                   <  1:153651/140‑1 (MQ=255)  cTGAAGCGCACTCGCCAATGCTGCCGATGTCTGTCTGTGTTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACt                                                                                                                                  <  2:397817/140‑1 (MQ=255)             tCGCCAATGCTGCCGATGTCTGTCTGTGTTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGt                                                                                                                       <  2:253983/140‑1 (MQ=255)                       tgcCGATGTCTGTCTGTGTTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAAc                                                                                                             >  1:320829/1‑140 (MQ=255)                         ccGATGTCTGTCTGTGTTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGa                                                                                                           >  1:701572/1‑140 (MQ=255)                               tctgtctgTGTTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGc                                                                                                     <  1:237618/140‑1 (MQ=255)                               tctgtctgTGTTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGc                                                                                                     <  2:838807/140‑1 (MQ=255)                                   tctgtGTTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTc                                                                                                 >  1:201997/1‑140 (MQ=255)                                   tctgtGTTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTc                                                                                                 >  1:344570/1‑140 (MQ=255)                                   tctgtGTTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTc                                                                                                 >  1:457201/1‑140 (MQ=255)                                       tgtTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGTTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCa                                                                                             <  1:773991/140‑1 (MQ=255)                                        gtTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCaa                                                                                            >  1:930443/1‑140 (MQ=255)                                         ttGCATTAAGCCAGGGTTATATTGGTTACCATCTACAAAGCGGTTTAAGAGAGGACCTGCTTTCTCGGGGCATTAATAAACGCGTAGCAACGCTGGCTACTCAGGTGGAAGTCGACGCTGACGATCCGGCATTCCTCAAc                                                                                           <  2:344570/140‑1 (MQ=255)                                          tGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAAcc                                                                                          >  2:809260/1‑140 (MQ=255)                                            cATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCg                                                                                        <  2:559578/140‑1 (MQ=255)                                             aTTAAGCCAGGGTTATATTGGTTACGATCTACAAACCGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGa                                                                                       >  1:743104/1‑140 (MQ=255)                                             aTTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGa                                                                                       <  2:457201/140‑1 (MQ=255)                                             aTTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGa                                                                                       <  2:201997/140‑1 (MQ=255)                                              ttAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGc                                                                                                     <  2:239842/125‑1 (MQ=255)                                              ttAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGc                                                                                                     >  1:239842/1‑125 (MQ=255)                                               tAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGAcc                                                                                     <  2:393317/140‑1 (MQ=255)                                                 aGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACGTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCaa                                                                                   >  2:834735/1‑140 (MQ=255)                                                 aGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCaa                                                                                            <  2:14215/131‑1 (MQ=255)                                                 aGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCaa                                                                                            >  1:14215/1‑131 (MQ=255)                                                 aGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCaa                                                                                   <  2:701572/140‑1 (MQ=255)                                                       ggTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGAt                                                                             >  2:515522/1‑140 (MQ=255)                                                           atatTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGgt                                                                         <  1:475788/140‑1 (MQ=255)                                                           atatTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGgt                                                                         <  1:32877/140‑1 (MQ=255)                                                            tatTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGgtt                                                                        >  1:377280/1‑140 (MQ=255)                                                                ggTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGgttcgtt                                                                    <  2:729024/140‑1 (MQ=255)                                                                 gTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGgttcgttc                                                                   >  1:304581/1‑140 (MQ=255)                                                                   tACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCtt                                                                 <  2:320829/140‑1 (MQ=255)                                                                            cAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCa                                                        <  1:809260/140‑1 (MQ=255)                                                                                    tttAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCag                                                >  2:953315/1‑140 (MQ=255)                                                                                           aaagaaCTGCTTTCTCGGGGCATTAATAACCCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACt                                         <  2:823838/139‑1 (MQ=255)                                                                                             agaaCTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCagag                                              >  1:850411/1‑133 (MQ=255)                                                                                             agaaCTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCagag                                              <  2:850411/133‑1 (MQ=255)                                                                                             agaaCTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGa                                       <  1:953315/140‑1 (MQ=255)                                                                                              gaaCTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGAc                                      >  1:455229/1‑140 (MQ=255)                                                                                              gaaCTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCT‑AACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGa                                       >  2:466831/1‑138 (MQ=255)                                                                                              gaaCTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCT‑AACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGa                                       <  1:466831/138‑1 (MQ=255)                                                                                                  tGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACaaaa                                  <  2:749352/140‑1 (MQ=255)                                                                                                  tGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACaaaa                                  <  1:515522/140‑1 (MQ=255)                                                                                                    cTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACa                                >  1:834546/1‑140 (MQ=255)                                                                                                    cTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACa                                <  1:563847/140‑1 (MQ=255)                                                                                                      ttCTCGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAgg                              >  2:190629/1‑140 (MQ=255)                                                                                                          cGGGGCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTa                          <  1:458833/140‑1 (MQ=255)                                                                                                            gggCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGTGCAAGAAGCAGAGCAACTGACAAAACAGGGTTaca                        <  1:953962/140‑1 (MQ=255)                                                                                                            gggCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTaca                        <  2:455229/140‑1 (MQ=255)                                                                                                              gCATTAATAAACCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTACAct                      <  1:672815/140‑1 (MQ=255)                                                                                                                       aaCCTGTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATTGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAGCTGACGACACAGGGCTACACTCTGAaagaa             >  2:672815/1‑140 (MQ=255)                                                                                                                            gTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTACACTCTGAAAGAAGATGc        >  1:642894/1‑140 (MQ=255)                                                                                                                            gTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACAATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTACACTCTGAaag               <  1:257296/133‑1 (MQ=255)                                                                                                                            gTAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACAATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTACACTCTGAaag               >  2:257296/1‑133 (MQ=255)                                                                                                                             tAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCt       >  2:608376/1‑140 (MQ=255)                                                                                                                             tAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCt       >  2:469004/1‑140 (MQ=255)                                                                                                                             tAGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCt       >  1:72538/1‑140 (MQ=255)                                                                                                                              aGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCTg      >  1:111632/1‑140 (MQ=255)                                                                                                                              aGCAACGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATAGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCTg      >  2:889478/1‑140 (MQ=255)                                                                                                                                  aCGCTGGCTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCTGGTcg  <  2:930443/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     ACTGAAGCGCACTCGCCAATGCTGCCGATGTCTGTCTGTGTTGCATTAAGCCAGGGTTATATTGGTTACGATCTACAAAACGCTTTAAGAGAAGAACTGCTTTCTCGGGGCATTAATAAACCTGTAGCAACGCTGGTTACTCAGGTCGAAGTCGACGCTAACGATCCGGCATTCCTCAACCCGACCAAGCCGATCGGTTCGTTCTTTACCGAGCAAGAAGCAGAGCAACTGACAAAACAGGGTTACACTCTGAAAGAAGATGCTGGTCG  >  NC_000913/3011653‑3011921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑