Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,105,207	T→C	F259L (TTC→CTC)	mltC →	membrane‑bound lytic murein transglycosylase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,105,207	0	T	C	100.0%	190.2 / NA	53	F259L (TTC→CTC)	mltC	membrane‑bound lytic murein transglycosylase C
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/28);  total (25/28)

TTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCG  >  NC_000913/3105070‑3105339                                                                                                                                          |                                                                                                                                     ttATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGtctc                                                                                                                                    <  2:931666/140‑1 (MQ=255)         aCCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGa                                                                                                                            <  1:553570/140‑1 (MQ=255)           cGAATCTTCCTTTAACACGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATc                                                                                                                          >  2:904317/1‑140 (MQ=255)             aaTCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCt                                                                                                                        <  2:790619/140‑1 (MQ=255)              aTCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTg                                                                                                                       >  2:45226/1‑140 (MQ=255)               tCTTCCTTTAACCCGTCTGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGc                                                                                                                      <  2:862426/140‑1 (MQ=255)               tCTTCCTTTAACACGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTgt                                                                                                                      <  1:904317/140‑2 (MQ=255)                  tCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAg                                                                                                                   <  2:614225/140‑1 (MQ=255)                    cTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCa                                                                                                                 <  1:879729/140‑1 (MQ=255)                        aaCCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAatat                                                                                                             >  1:481249/1‑140 (MQ=255)                         aCCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATAtt                                                                                                            <  2:383047/140‑1 (MQ=255)                               aTGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATAcc                                                                                                      >  1:833925/1‑140 (MQ=255)                                 gCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCgg                                                                                                    <  2:662303/140‑1 (MQ=255)                                    gTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCAc                                                                                                 >  2:373064/1‑140 (MQ=255)                                     tCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCAcc                                                                                                <  2:833925/140‑1 (MQ=255)                                       aGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACcgc                                                                                              <  1:373064/140‑1 (MQ=255)                                       aGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACcgc                                                                                              >  2:806911/1‑140 (MQ=255)                                        gCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGc                                                                                                   <  2:499321/134‑1 (MQ=255)                                        gCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGc                                                                                                   >  1:499321/1‑134 (MQ=255)                                        gCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACcgcg                                                                                             >  2:433834/1‑140 (MQ=255)                                        gCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACcgcg                                                                                             >  2:309546/1‑140 (MQ=255)                                         ccGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTTATACCGGCACCGCGt                                                                                            >  1:563930/1‑140 (MQ=255)                                            ttCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATc                                                                                         >  2:800169/1‑140 (MQ=255)                                              ccGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTg                                                                                       >  1:9218/1‑140 (MQ=255)                                               cGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTgg                                                                                      <  1:901527/140‑1 (MQ=255)                                                 aTGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCg                                                                                    <  1:230002/140‑1 (MQ=255)                                                 aTGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCg                                                                                    >  2:546329/1‑140 (MQ=255)                                                         ggATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGaa                                                                            <  1:546329/140‑1 (MQ=255)                                                         ggATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGaa                                                                            <  2:9218/140‑1 (MQ=255)                                                              aaTGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATg                                                                       >  2:558531/1‑140 (MQ=255)                                                                   aGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTAt                                                                  <  1:80971/140‑1 (MQ=255)                                                                   aGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTAt                                                                  <  2:572699/140‑1 (MQ=255)                                                                     gtggtACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTAtct                                                                <  1:806911/140‑1 (MQ=255)                                                                     gtggtACAACATACTGCCGGGAAAGATATGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTAtct                                                                >  2:419411/1‑140 (MQ=255)                                                                       ggtACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTcg                                                              >  1:133632/1‑140 (MQ=255)                                                                        gtACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTcgg                                                             <  1:800169/140‑1 (MQ=255)                                                                        gtACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTcgg                                                             <  1:433834/140‑1 (MQ=255)                                                                         tACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTcggc                                                            >  1:594108/1‑140 (MQ=255)                                                                         tACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGGCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTcggc                                                            >  1:128986/1‑140 (MQ=255)                                                                                 aCTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGTAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGa                                                    >  2:253028/1‑140 (MQ=255)                                                                                 aCTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGa                                                    >  1:729515/1‑140 (MQ=255)                                                                                  cTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGAt                                                   <  1:51215/140‑1 (MQ=255)                                                                                   tGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATa                                                  >  1:253564/1‑140 (MQ=255)                                                                                   tGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATa                                                  >  1:182534/1‑140 (MQ=255)                                                                                     ccGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATaa                                                 <  2:126096/139‑1 (MQ=255)                                                                                     ccGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATaa                                                 >  1:126096/1‑139 (MQ=255)                                                                                        ggAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCa                                             <  1:309546/140‑1 (MQ=255)                                                                                                gtgtTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATcgcg                                     <  2:729515/140‑1 (MQ=255)                                                                                                 tgtTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCgcgg                                    <  1:527641/140‑1 (MQ=255)                                                                                                   ttCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCgcggcg                                  >  1:721620/1‑140 (MQ=255)                                                                                                   ttCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCgcggcg                                  <  1:419411/140‑1 (MQ=255)                                                                                                        cTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATg                             <  2:133632/140‑1 (MQ=255)                                                                                                         tCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGc                            <  1:558531/140‑1 (MQ=255)                                                                                                                           aCGCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATaa          >  1:299611/1‑140 (MQ=255)                                                                                                                             gCCGAGCCGCAGTCTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGAAATTGATACCCCAACATCGGGGCTTTATGCCGTCATCACCGCCTATAAcg        <  2:594108/140‑1 (MQ=255)                                                                                                                                   ccGCATTTTTTTGTTTGACCCTGCCAGCAATATGGATCCCGGCCCCGCGTATCTGGCGATGCTGAACAATGTTTATCTGGGCGGAATTGAAACCCCAACACCGCGGCTTTATGCCGTCATCACCGCCTATAACGgcggcg  <  2:498593/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     TTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCG  >  NC_000913/3105070‑3105339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑