Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,230,215	T→C	V443A (GTG→GCG)	ygjK →	glycoside hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,230,215	0	T	C	100.0%	178.6 / NA	51	V443A (GTG→GCG)	ygjK	glycoside hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/19);  total (32/19)

TAGCCCCGAGCGTGGTGGCGATGGCGGCAACTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGTGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAA  >  NC_000913/3230078‑3230351                                                                                                                                          |                                                                                                                                         tAGCCCCGAGCGTGGTGGCGATGGCGGCAACTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACtggcgg                                                                                                                                        >  1:140908/1‑140 (MQ=255)  aGCCCCGAGCGTGGTGGCGATGGCGGCAACTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTggcggc                                                                                                                                       <  2:189149/140‑1 (MQ=255)         aGCGTGGTGGCGATGGCGGCAACTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCAc                                                                                                                                >  1:924201/1‑140 (MQ=255)             tggtggCGATGGCGGCAACTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACt                                                                                                                            >  2:359748/1‑140 (MQ=255)                          ggcAACTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACtg                                                                                                                           <  2:782286/128‑1 (MQ=255)                          ggcAACTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACtg                                                                                                                           >  1:782286/1‑128 (MQ=255)                          ggcAACTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTaa                                                                                                               >  1:682384/1‑140 (MQ=255)                             aaCTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACcg                                                                                                            >  1:32668/1‑140 (MQ=255)                                  gaacgaacGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATc                                                                                                       <  1:919846/140‑1 (MQ=255)                                  gaacgaacGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATc                                                                                                       <  2:682384/140‑1 (MQ=255)                                                 cAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCg                                                                                        <  2:140908/140‑1 (MQ=255)                                                 cAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCg                                                                                        <  2:924201/140‑1 (MQ=255)                                                    aCCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGc                                                                                     >  1:67711/1‑140 (MQ=255)                                                      ccAGCCTTGCCGCCTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCg                                                                                   >  2:724841/1‑140 (MQ=255)                                                        aGCCTTGCCGCCTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCAcaacggcaacgg                                                                                          <  2:897115/131‑1 (MQ=255)                                                        aGCCTTGCCGCCTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCAcaacggcaacgg                                                                                          >  1:897115/1‑131 (MQ=255)                                                        aGCCTTGCCGCCTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCg                                                                                   <  1:723398/138‑1 (MQ=255)                                                        aGCCTTGCCGCCTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCg                                                                                   >  2:723398/1‑138 (MQ=255)                                                        aGCCTTGCCGCCTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGa                                                                                 <  2:449914/140‑1 (MQ=255)                                                        aGCCTTGCCGCCTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGa                                                                                 >  1:919739/1‑140 (MQ=255)                                                              gccgccTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATgg                                                                           >  1:731177/1‑140 (MQ=255)                                                               ccgccTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGgc                                                                          >  2:31681/1‑140 (MQ=255)                                                               ccgccTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGgc                                                                          >  2:928088/1‑140 (MQ=255)                                                                            gATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCcg                                                                  <  2:912674/135‑1 (MQ=255)                                                                            gATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCcg                                                                  >  1:912674/1‑135 (MQ=255)                                                                                 aaGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGcc                                                        <  2:839704/140‑1 (MQ=255)                                                                                  aGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGccc                                                       <  1:928088/140‑1 (MQ=255)                                                                                  aGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGccc                                                       <  1:31681/140‑1 (MQ=255)                                                                                  aGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGccc                                                       <  2:32668/140‑1 (MQ=255)                                                                                     gtATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCcaca                                                    <  2:154334/140‑1 (MQ=255)                                                                                      tataACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCacaa                                                   >  2:339671/1‑140 (MQ=255)                                                                                           cGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTg                                              >  2:163483/1‑140 (MQ=255)                                                                                              cACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTgaga                                           >  1:941169/1‑140 (MQ=255)                                                                                              cACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTgaga                                           >  1:28174/1‑140 (MQ=255)                                                                                              cACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTgaga                                           >  1:62507/1‑140 (MQ=255)                                                                                              cACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTgaga                                           >  2:244624/1‑140 (MQ=255)                                                                                                    aGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAgcggcg                                     <  1:244624/140‑1 (MQ=255)                                                                                                               gggTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGttt                          >  2:630293/1‑140 (MQ=255)                                                                                                                ggTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTa                         >  1:532644/1‑140 (MQ=255)                                                                                                                 gTGGCAGAGATGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTAc                        >  2:772195/1‑140 (MQ=255)                                                                                                                          aTGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGaaaaa               >  2:325167/1‑140 (MQ=255)                                                                                                                           tGTACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGaaaaaa              <  1:683054/140‑1 (MQ=255)                                                                                                                             tACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAgg            <  2:532644/140‑1 (MQ=255)                                                                                                                             tACCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAgg            <  2:253282/140‑1 (MQ=255)                                                                                                                              aCCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACg                       >  1:7086/1‑128 (MQ=255)                                                                                                                              aCCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACg                       <  2:7086/128‑1 (MQ=255)                                                                                                                              aCCCGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGc           >  2:303208/1‑140 (MQ=255)                                                                                                                               cccGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCg          >  2:272994/1‑140 (MQ=255)                                                                                                                               cccGAAACTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCg          >  1:801943/1‑140 (MQ=255)                                                                                                                                   aaaCTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATaa      >  1:342630/1‑140 (MQ=255)                                                                                                                                    aaCTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATaaa     >  2:950074/1‑140 (MQ=255)                                                                                                                                    aaCTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATaaa     >  1:529772/1‑140 (MQ=255)                                                                                                                                     aCTGGCGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAaag    >  1:237020/1‑140 (MQ=255)                                                                                                                                       tggcggCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAaagaa  <  1:325167/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         TAGCCCCGAGCGTGGTGGCGATGGCGGCAACTGGAACGAACGTAATACCAAACCCAGCCTTGCCGCCTGGTCGGTGATGGAAGTGTATAACGTCACCCAAGATAAAACCTGGGTGGCAGAGATGTACCCGAAACTGGTGGCCTATCACGACTGGTGGTTACGTAACCGCGATCACAACGGCAACGGCGTGCCGGAATATGGCGCGACCCGCGACAAAGCCCACAACACTGAGAGCGGCGAGATGCTGTTTACGGTGAAAAAAGGCGATAAAGAA  >  NC_000913/3230078‑3230351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑