Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,267,951	T→C	V33A (GTA→GCA)	yhaB →	protein YhaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,267,951	0	T	C	100.0%	216.2 / NA	60	V33A (GTA→GCA)	yhaB	protein YhaB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/26);  total (34/26)

TTATTGTTTGTCATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATG  >  NC_000913/3267821‑3268084                                                                                                                                    |                                                                                                                                      ttattGTTTGTCATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAaaagaaa                                                                                                                              >  2:7363/1‑140 (MQ=255)       tttgtCATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGc                                                                                                                        >  1:539311/1‑140 (MQ=255)        ttgtCATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCa                                                                                                                       >  1:950200/1‑140 (MQ=255)         tgtCATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAg                                                                                                                      >  1:619337/1‑140 (MQ=255)         tgtCATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAg                                                                                                                      >  2:71370/1‑140 (MQ=255)         tgtCATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAg                                                                                                                      >  2:368062/1‑140 (MQ=255)         tgtCATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAg                                                                                                                      >  1:355546/1‑140 (MQ=255)           tCATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGaa                                                                                                                    >  2:746874/1‑140 (MQ=255)           tCATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGaa                                                                                                                    >  1:430933/1‑140 (MQ=255)            cATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGaaa                                                                                                                   <  1:71370/140‑1 (MQ=255)            cATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGaaa                                                                                                                   <  1:502767/140‑1 (MQ=255)                 tgaCAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCCCCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGtc                                                                                                              <  2:208156/140‑1 (MQ=255)                 tgaCAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGtc                                                                                                              <  1:385887/140‑1 (MQ=255)                 tgaCAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGtc                                                                                                              >  2:385887/1‑140 (MQ=255)                   aCAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGtctc                                                                                                            <  2:918230/140‑1 (MQ=255)                    cAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTcc                                                                                                           <  1:368062/140‑1 (MQ=255)                     aaGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTcca                                                                                                          >  1:709249/1‑140 (MQ=255)                          aggcaTCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGtctc                                                                                                            >  1:748244/1‑133 (MQ=255)                          aggcaTCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGtctc                                                                                                            <  2:748244/133‑1 (MQ=255)                          aggcaTCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAAt                                                                                                     <  2:6219/140‑1 (MQ=255)                                   tggaaGGCTTTCCCAATGGGCATATTTTTCACCCTTTAATTCCGGCCAAGGC‑CGCAGATGGTAACAAACACAATAGAAATATTGATTATTTGGCGGCCAAAAGAAAGGTTGCAGAAAATGTCTCCACCAATGACGTtaat                                                                                            >  2:666714/4‑140 (MQ=255)                                      aaGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATaaa                                                                                         >  2:462292/1‑140 (MQ=255)                                      aaGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATaaa                                                                                         <  1:462292/140‑1 (MQ=255)                                       aGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATaaaa                                                                                        >  2:519455/1‑140 (MQ=255)                                          cTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAAttt                                                                                     <  2:234386/140‑1 (MQ=255)                                          cTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGACTCCACCAATGACGTTAATAAAAttt                                                                                     <  1:389560/140‑1 (MQ=255)                                           ttCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTa                                                                                    >  1:217372/1‑140 (MQ=255)                                             cccAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACa                                                                                  >  2:459162/1‑140 (MQ=255)                                               cAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGt                                                                                <  1:666714/140‑1 (MQ=255)                                                 aTTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGtat                                                                              <  1:746874/140‑1 (MQ=255)                                                     cgcATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGt                                                                          >  1:775535/1‑140 (MQ=255)                                                     cgcATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGt                                                                          >  2:611807/1‑140 (MQ=255)                                                      gcATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTa                                                                         <  1:611807/140‑1 (MQ=255)                                                       cATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTaa                                                                        >  1:641777/1‑140 (MQ=255)                                                           tttttCCCCCTTAAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGaa                                                                    <  2:298895/140‑1 (MQ=255)                                                             tttCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACt                                                                  <  2:539311/140‑1 (MQ=255)                                                                cACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCg                                                               <  2:355546/140‑1 (MQ=255)                                                                cACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCg                                                               <  2:266189/140‑1 (MQ=255)                                                                 aCCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCgg                                                              <  2:709249/140‑1 (MQ=255)                                                                 aCCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCgg                                                              <  1:519455/140‑1 (MQ=255)                                                                 aCCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCgg                                                              <  2:775535/140‑1 (MQ=255)                                                                  cccTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGga                                                             <  2:217372/140‑1 (MQ=255)                                                                              cGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCt                                                  >  1:553417/1‑140 (MQ=255)                                                                                ccAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGc                                                >  2:951711/1‑140 (MQ=255)                                                                                            gTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCAtt                                    <  2:641777/140‑1 (MQ=255)                                                                                              ggTTAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGTAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCTGAGAGTATTATCTGCAATAACTGCATTGa                                  >  1:386873/1‑140 (MQ=255)                                                                                                ttAACAATCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAAt                                >  1:906969/1‑140 (MQ=255)                                                                                                       tCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATc                         >  1:166627/1‑140 (MQ=255)                                                                                                       tCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATc                         >  1:932512/1‑140 (MQ=255)                                                                                                       tCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATc                         >  1:330574/1‑140 (MQ=255)                                                                                                       tCACAATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATc                         >  2:364133/1‑140 (MQ=255)                                                                                                         acaATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCAt                       >  1:939697/1‑140 (MQ=255)                                                                                                         acaATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCAt                       >  1:747855/1‑140 (MQ=255)                                                                                                         acaATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCAt                       >  2:372278/1‑140 (MQ=255)                                                                                                          caATAGAAATATTGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATg                      <  2:330574/140‑1 (MQ=255)                                                                                                                      tGATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCtt          <  2:747855/140‑1 (MQ=255)                                                                                                                       gATTATTGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTc         >  2:479287/1‑140 (MQ=255)                                                                                                                          tattGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTAAAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGt      >  2:372738/1‑140 (MQ=255)                                                                                                                             tGGACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCAt   <  1:372278/140‑1 (MQ=255)                                                                                                                             tGGACGGCAAAAAGAAAGTTTGCAGAAATCGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCAt   >  2:137828/1‑140 (MQ=255)                                                                                                                              ggACGGCAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCAtt  >  2:279544/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                      TTATTGTTTGTCATGATGACAAGGCAGGCATCAATGAAAGGCTTCCCAATTGCGCATATTTTTCACCCTTCAA‑TCCCGCCAATGCACGCAGTGGTTAACAATCACAATAGAAATATTGATTATTGGACGGTAAAAAGAAAGTTTGCAGAAATTGTCTCCACCAATGACGTTAATAAAATTTACAGTATAAGTAATGAACTGCGGAGAGTATTATCTGCAATAACTGCATTGAATTTCTATCATGGCGATGTTCCTTCTGTCATG  >  NC_000913/3267821‑3268084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑