Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,284,795 T→C C37C (TGT→TGC)  agaC → galactosamine‑specific PTS enzyme IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,284,7950TC100.0% 162.7 / NA 46C37C (TGT→TGC) agaCgalactosamine‑specific PTS enzyme IIC component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/22);  total (24/22)

CCTTAATATTGGTTTGAGGTAATAAAAATGCATGAAATAACCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGTACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCA  >  NC_000913/3284658‑3284931
                                                                                                                                         |                                                                                                                                        
ccTTAATATTGGTTTGAGGTAATAAAAATGCATGAAATAACCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCAc                                                                                                                                        >  1:56250/1‑140 (MQ=255)
           gTTTGAGGTAATAAAAATGCATGAAATAACCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTGTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGgcg                                                                                                                             <  2:955366/140‑1 (MQ=255)
           gTTTGAGGTAATAAAAATGCATGAAATAACCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGgcg                                                                                                                             >  1:516487/1‑140 (MQ=255)
               gAGGTAATAAAAATGCATGAAATAACCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTAt                                                                                                                         >  1:951826/1‑140 (MQ=255)
                  gTAATAAAAATGCATGAAATAACCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATtct                                                                                                                      <  2:238360/140‑1 (MQ=255)
                      taaAAATGCATGAAATAACCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGt                                                                                                                  <  1:608093/140‑1 (MQ=255)
                       aaaaaTGCATGAAATAACCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTg                                                                                                                 <  1:705887/140‑1 (MQ=255)
                                tGAAATAACCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGa                                                                                                        <  2:910630/140‑1 (MQ=255)
                                   aaTAACCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTg                                                                                                     <  2:56250/140‑1 (MQ=255)
                                     tAACCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGc                                                                                                   <  2:516487/140‑1 (MQ=255)
                                      aaCCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCt                                                                                                  <  2:168964/140‑1 (MQ=255)
                                        cccTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTa                                                                                                >  2:67471/1‑140 (MQ=255)
                                                cAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCgg                                                                                        <  2:757838/140‑1 (MQ=255)
                                                  gggATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCggtg                                                                                      >  2:66748/1‑140 (MQ=255)
                                                  gggATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCggtg                                                                                      >  1:800880/1‑140 (MQ=255)
                                                   ggATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCggtgg                                                                                     >  1:272983/1‑140 (MQ=255)
                                                   ggATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACGGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCggtgg                                                                                     >  2:634459/1‑140 (MQ=255)
                                                        aTCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGa                                                                                <  1:947458/140‑1 (MQ=255)
                                                        aTCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGa                                                                                >  2:111127/1‑140 (MQ=255)
                                                        aTCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGa                                                                                >  1:609510/1‑140 (MQ=255)
                                                        aTCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGa                                                                                >  1:287043/1‑140 (MQ=255)
                                                        aTCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCACTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGa                                                                                >  1:739477/1‑140 (MQ=255)
                                                             tGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACagag                                                                           >  1:709269/1‑140 (MQ=255)
                                                             tGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACagag                                                                           <  2:287043/140‑1 (MQ=255)
                                                               gcggcgTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGtt                                                                         >  1:137206/1‑140 (MQ=255)
                                                                     ttAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCttt                                                                   >  1:208352/1‑140 (MQ=255)
                                                                       aGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCg                                                                 <  1:67471/140‑1 (MQ=255)
                                                                        gTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGc                                                                <  2:153452/140‑1 (MQ=255)
                                                                         tttttGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGcc                                                               <  2:636724/140‑1 (MQ=255)
                                                                             tGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCg                                                                 <  2:620820/134‑1 (MQ=255)
                                                                             tGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCg                                                                 >  1:620820/1‑134 (MQ=255)
                                                                               ttCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGcc                                                               <  1:750730/134‑1 (MQ=255)
                                                                               ttCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGcc                                                               >  2:750730/1‑134 (MQ=255)
                                                                               ttCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTa                                                         >  1:189072/1‑140 (MQ=255)
                                                                                 cTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCACTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAAc                                                       <  2:739477/140‑1 (MQ=255)
                                                                                  tGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAAcc                                                      >  1:76311/1‑140 (MQ=255)
                                                                                   ggggATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAAccc                                                     >  1:572441/1‑140 (MQ=255)
                                                                                   ggggATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAAccc                                                     >  2:417767/1‑140 (MQ=255)
                                                                                          gattTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAgg                                              <  1:560857/140‑1 (MQ=255)
                                                                                          gattTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAgg                                              <  2:272983/140‑1 (MQ=255)
                                                                                                                  ttATTCCGCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGAt                      <  2:467774/140‑1 (MQ=255)
                                                                                                                         gCCCGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCg               >  1:877185/1‑140 (MQ=255)
                                                                                                                          cccGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCgg              >  2:146257/1‑140 (MQ=255)
                                                                                                                          cccGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCgg              >  2:139161/1‑140 (MQ=255)
                                                                                                                          cccGATAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCgg              <  1:139161/140‑1 (MQ=255)
                                                                                                                               tAATCGTTTGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCtg         <  1:111127/140‑1 (MQ=255)
                                                                                                                                      ttGCACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGacca  <  1:66748/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
CCTTAATATTGGTTTGAGGTAATAAAAATGCATGAAATAACCCTACTTCAGGGATTATCCCTGGCGGCGTTAGTTTTTGTTCTGGGGATTGATTTTTGGCTGGAAGCCTTATTTTTATTCCGCCCGATAATCGTTTGTACCCTAACTGGCGCTATTCTCGGTGATATTCAGACTGGCTTAATTACCGGTGGTCTGACAGAGTTGGCTTTCGCCGGATTAACCCCTGCAGGTGGTGTTCAGCCGCCCAACCCGATTATGGCGGGTCTGATGACCA  >  NC_000913/3284658‑3284931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑