Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	392,964	T→A	intergenic (‑63/‑6)	insE‑2 ← / → yaiT	IS3 element protein InsE/putative autotransporter YaiT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	392,964	0	T	A	100.0%	163.2 / NA	49	intergenic (‑63/‑6)	insE‑2/yaiT	IS3 element protein InsE/putative autotransporter YaiT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (32/17);  total (32/17)

GCTTCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTGAAGCTTTCCGTACTGACAAACTGGAACTGACCAGCGGCAACATCGCTGACCATAA  >  NC_000913/392834‑393100                                                                                                                                   |                                                                                                                                         gCTTCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACa                                                                                                                                 <  1:271974/140‑1 (MQ=11) gCTTCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACa                                                                                                                                 <  2:600746/140‑1 (MQ=11)   ttCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAAc                                                                                                                               >  1:3840/1‑140 (MQ=17)   ttCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAAc                                                                                                                               >  1:957023/1‑140 (MQ=17)    tCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAAcc                                                                                                                              >  1:862879/1‑140 (MQ=18)     cACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCt                                                                                                                             >  1:213016/1‑140 (MQ=18)      aCTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTg                                                                                                                            <  1:752841/140‑1 (MQ=21)                  aGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGcc                                                                                                                      <  2:878996/134‑1 (MQ=255)                  aGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGcc                                                                                                                      >  1:878996/1‑134 (MQ=255)                              tttACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTa                                                                                                               >  2:732485/1‑129 (MQ=255)                              tttACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTa                                                                                                               <  1:732485/129‑1 (MQ=255)                                  cGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAAcg                                                                                                >  1:786589/1‑140 (MQ=255)                                   ggggTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACATCCTGTACGCCAATACTATCACCGTAACTAAcgg                                                                                               <  2:213016/140‑1 (MQ=255)                                     ggTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAAcggcg                                                                                             >  2:676624/1‑140 (MQ=255)                                        tttttACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGgtg                                                                                          >  1:533577/1‑140 (MQ=255)                                        tttttACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGgtg                                                                                          <  2:533577/140‑1 (MQ=255)                                                ggTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGAt                                                                                  <  2:786589/140‑1 (MQ=255)                                                  ttGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGAtgt                                                                                >  1:770836/1‑140 (MQ=255)                                                       aCTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACg                                                                           <  2:862879/140‑1 (MQ=255)                                                           tttttGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGa                                                                       >  2:224861/1‑140 (MQ=255)                                                             tttGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGAtgt                                                                                >  2:374090/1‑129 (MQ=255)                                                             tttGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGAtgt                                                                                <  1:374090/129‑1 (MQ=255)                                                                  cATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTc                                                                <  1:224861/140‑1 (MQ=255)                                                                     gtgAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGAt                                                             >  1:597704/1‑140 (MQ=255)                                                                         gTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTg                                                         >  1:661518/1‑140 (MQ=255)                                                                          tCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTGa                                                        <  1:606240/140‑1 (MQ=255)                                                                               tctGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTGAAGCtt                                                   >  2:549720/1‑140 (MQ=255)                                                                                   actgaGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTGAAGCTTTCCg                                               >  1:77340/1‑140 (MQ=255)                                                                                   actgaGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTGAAGCTTTCCg                                               >  1:96064/1‑140 (MQ=255)                                                                                   actgaGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTGAAGCTTTCCg                                               <  1:639909/140‑1 (MQ=255)                                                                                    ctgaGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTGAAGCTTTCCGt                                              >  2:506255/1‑140 (MQ=255)                                                                                        gagTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGGTCAGTTCGATACTGAAGCTTTCCGTACTg                                          >  2:568118/1‑140 (MQ=255)                                                                                        gagTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTGAAGCTTTCCGTACTg                                          >  2:753581/1‑140 (MQ=255)                                                                                         agTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTGAAGCTTTCCGTACTGa                                         <  2:3840/140‑1 (MQ=255)                                                                                                    ttAGCCGCGTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTGAAGCTTTCCGTACTGACAAACTGGAAc                              >  1:869259/1‑140 (MQ=255)                                                                                                         cgcgTGTCCACTATTGCCGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTGAAGCTTTCCGTACTGACAAACTGGAACTGAcc                         >  2:916735/1‑140 (MQ=255)                                                                                                          gcgTGTCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTGAAGCTTTCCGTACTGACAAACTGGAACTGACCa                        >  1:404513/1‑140 (MQ=255)                                                                                                            gtgtCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTGAAGCTTTCCGTACTGACAAACTGGAACTGAcc                         <  2:114254/137‑1 (MQ=255)                                                                                                            gtgtCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTGAAGCTTTCCGTACTGACAAACTGGAACTGAcc                         <  2:701315/137‑1 (MQ=255)                                                                                                            gtgtCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTGAAGCTTTCCGTACTGACAAACTGGAACTGAcc                         >  1:701315/1‑137 (MQ=255)                                                                                                            gtgtCCACTATTGCTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTGAAGCTTTCCGTACTGACAAACTGGAACTGAcc                         >  1:114254/1‑137 (MQ=255)                                                                                                                         cTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTGAAGCTTTCCGTACTGACAAACTGGAACTGACCAGCGGCAACATCGCTg         >  1:537580/1‑140 (MQ=255)                                                                                                                         cTGGGTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTGAAGCTTTCCGTACTGACAAACTGGAACTGACCAGCGGCAACATCGCTg         >  1:410956/1‑140 (MQ=255)                                                                                                                           gggTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTGAAGCTTTCCGTACTGACAAACTGGAACTGACCAGCGGCAACATCGCTGAc       >  1:898508/1‑140 (MQ=255)                                                                                                                            ggTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTGAAGCTTTCCGTACTGACAAACTGGAACTGACCAGCGGCAACATCGCTGAcc      >  2:242702/1‑140 (MQ=255)                                                                                                                            ggTAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTGAAGCTTTCCGTACTGACAAACTGGAACTGACCAGCGGCAACATCGCTGAcc      >  2:198210/1‑140 (MQ=255)                                                                                                                              tAAGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTGAAGCTTTCCGTACTGACAAACTGGAACTGACCAGCGGCAACATCGCTGACCAt    >  1:950162/1‑140 (MQ=255)                                                                                                                                aGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTGAAGCTTTCCGTACTGACAAACTGGAACTGACCAGCGGCAACATCGCTGACCATaa  >  1:860828/1‑140 (MQ=255)                                                                                                                                aGAACAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTGAAGCTTTCCGTACTGACAAACTGGAACTGACCAGCGGCAACATCGCTGACCATaa  <  1:198210/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         GCTTCACTGCGAAATTCAGGCGAATGCTGTTTACGGGGTTTTTTACTGGTTGATACTGTTTTTGTCATGTGAGTCACCTCTGACTGAGAGTTTACTCACTTAGCCGCGTGTCCACTATTGCTGGGTAAGATCAGATTACAACCTGTACGCCAATACTATCACCGTAACTAACGGCGGTGTATTGGATGTGAACGTTGATCAGTTCGATACTGAAGCTTTCCGTACTGACAAACTGGAACTGACCAGCGGCAACATCGCTGACCATAA  >  NC_000913/392834‑393100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑