Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,546,188	T→C	S189P (TCC→CCC)	nfuA →	iron‑sulfur cluster carrier protein NfuA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,546,188	0	T	C	100.0%	126.9 / NA	36	S189P (TCC→CCC)	nfuA	iron‑sulfur cluster carrier protein NfuA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/20);  total (16/20)

CAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACTCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTT  >  NC_000913/3546053‑3546313                                                                                                                                        |                                                                                                                              cAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACAcccc                                                                                                                             >  1:10761/1‑138 (MQ=255)    tttGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCctacta                                                                                                                        >  1:719703/1‑140 (MQ=255)      tGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCctactact                                                                                                                      <  2:745360/140‑1 (MQ=255)      tGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCctactact                                                                                                                      <  1:394491/140‑1 (MQ=255)      tGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCAAACACCAGCGCGGCGAACACCCctactact                                                                                                                      >  2:111227/1‑140 (MQ=255)           gcggcTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGtt                                                                                                                 >  2:41481/1‑140 (MQ=255)                tGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATccc                                                                                                            <  1:114121/140‑1 (MQ=255)                tGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATccc                                                                                                            <  2:885162/140‑1 (MQ=255)                tGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATccc                                                                                                            <  2:31826/140‑1 (MQ=255)                  tAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCct                                                                                                          <  1:173378/140‑1 (MQ=255)                  tAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCct                                                                                                          <  2:753853/140‑1 (MQ=255)                  tAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCct                                                                                                          <  2:255527/140‑1 (MQ=255)                  tAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCct                                                                                                          <  1:773917/140‑1 (MQ=255)                  tAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCAAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCct                                                                                                          <  1:111227/140‑1 (MQ=255)                       gttgttCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCCTCtttt                                                                                                     <  2:120830/140‑1 (MQ=255)                            tCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCCTCTTTTGTAGa                                                                                                <  1:800669/140‑1 (MQ=255)                                      aTGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATg                                                                                      <  2:211995/140‑1 (MQ=255)                                                 aaaGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAg                                                                           >  2:284224/1‑140 (MQ=255)                                                           tCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCg                                                                 >  1:322974/1‑140 (MQ=255)                                                           tCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCg                                                                 >  2:528496/1‑140 (MQ=255)                                                              agaAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGc                                                              <  2:322974/140‑1 (MQ=255)                                                                      ctgctgAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTg                                                      >  1:210878/1‑140 (MQ=255)                                                                        gctgAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGtt                                                       >  1:560669/1‑137 (MQ=255)                                                                        gctgAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGtt                                                       <  2:560669/137‑1 (MQ=255)                                                                        gctgAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGaa                                                    >  1:641149/1‑140 (MQ=255)                                                                                aaTTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGcc                                            >  2:933242/1‑140 (MQ=255)                                                                                   tCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGa                                         <  1:41481/140‑1 (MQ=255)                                                                                          gCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACATTGgc                                  <  1:933242/140‑1 (MQ=255)                                                                                              aaaGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCgcag                              >  2:394046/1‑140 (MQ=255)                                                                                               aaGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCgcagc                             <  1:394046/140‑1 (MQ=255)                                                                                                   tgtgCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTg                         <  2:558439/140‑1 (MQ=255)                                                                                                   tgtgCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTg                         >  2:876541/1‑140 (MQ=255)                                                                                                     tgCGCGATCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCt                       >  2:647524/1‑140 (MQ=255)                                                                                                           aTCTCACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCa                 <  2:641149/140‑1 (MQ=255)                                                                                                            tctcACCGAACACCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCaa                >  2:663581/1‑140 (MQ=255)                                                                                                                      cacCAGCGCGGCGAACACCCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGtt      >  1:536595/1‑140 (MQ=255)                                                                                                                          aGCGCGGCGAACACCCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGtt  >  1:68743/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                              CAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGCTGAAAGAAGGGATCGAGAAGCAGCTGCTGAACGAATTCCCGGAGCTGAAAGGTGTGCGCGATCTCACCGAACACCAGCGCGGCGAACACTCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTT  >  NC_000913/3546053‑3546313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑