Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,546,376	A→G	intergenic (+177/‑183)	nfuA → / → gntT	iron‑sulfur cluster carrier protein NfuA/high‑affinity gluconate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,546,376	0	A	G	100.0%	195.7 / NA	55	intergenic (+177/‑183)	nfuA/gntT	iron‑sulfur cluster carrier protein NfuA/high‑affinity gluconate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/32);  total (23/32)

CGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGAT  >  NC_000913/3546241‑3546497                                                                                                                                        |                                                                                                                          cGTAATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGcc                                                                                                                       <  1:381252/140‑1 (MQ=255)   tCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAg                                                                                                                     >  1:115308/1‑140 (MQ=255)   tCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAg                                                                                                                     >  2:425485/1‑140 (MQ=255)     aTTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGAt                                                                                                                   <  1:894469/140‑1 (MQ=255)          gggCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTAc                                                                                                              >  2:930201/1‑140 (MQ=255)          gggCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTAc                                                                                                              <  1:859602/140‑1 (MQ=255)             ccTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCg                                                                                                           <  2:646621/140‑1 (MQ=255)             ccTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCg                                                                                                           <  1:284138/140‑1 (MQ=255)                aCGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTAt                                                                                                        >  1:336317/1‑140 (MQ=255)                aCGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTAt                                                                                                        >  1:711757/1‑140 (MQ=255)                 cGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTAc                                                                                                              >  2:863915/1‑133 (MQ=255)                 cGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTAc                                                                                                              <  1:863915/133‑1 (MQ=255)                      aaCATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATg                                                                                                                  >  2:330055/1‑124 (MQ=255)                      aaCATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTca                                                                                                  >  2:615292/1‑140 (MQ=255)                      aaCATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTca                                                                                                  >  1:4362/1‑140 (MQ=255)                      aaCATGTGCCAGACGTTGGCGCAGCATTGATTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATg                                                                                                                  <  1:330055/124‑1 (MQ=255)                         aTGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACAt                                                                                               <  1:668240/140‑1 (MQ=255)                           gtgCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTcaca                                                                                                >  2:576810/1‑137 (MQ=255)                           gtgCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTcaca                                                                                                <  1:576810/137‑1 (MQ=255)                           gtgCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACAt                                                                                               <  2:944086/138‑1 (MQ=255)                           gtgCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACAt                                                                                               >  1:944086/1‑138 (MQ=255)                           gtgCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATgg                                                                                             <  1:464157/140‑1 (MQ=255)                           gtgCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATgg                                                                                             >  2:464157/1‑140 (MQ=255)                           gtgCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATgg                                                                                             <  2:336317/140‑1 (MQ=255)                           gtgCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATgg                                                                                             >  2:273636/1‑140 (MQ=255)                             gCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGt                                                                                           <  2:115308/140‑1 (MQ=255)                               cAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTAc                                                                                         <  1:659986/140‑1 (MQ=255)                               cAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTAc                                                                                         <  2:719703/140‑1 (MQ=255)                                aGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTAcc                                                                                        <  1:938419/140‑1 (MQ=255)                                aGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTAcc                                                                                        <  2:194315/140‑1 (MQ=255)                                aGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTAcc                                                                                        <  1:273636/140‑1 (MQ=255)                                       ggCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATa                                                                                 <  1:615292/140‑1 (MQ=255)                                            agcaTTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCt                                                                            <  1:284224/140‑1 (MQ=255)                                                 tGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACAt                                                                       <  1:76449/140‑1 (MQ=255)                                                  gCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATc                                                                      >  2:464414/1‑140 (MQ=255)                                                      tGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGAc                                                                  <  2:711757/140‑1 (MQ=255)                                                      tGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGAc                                                                  <  2:4362/140‑1 (MQ=255)                                                        tCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTa                                                                >  2:160345/1‑140 (MQ=255)                                                               cTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACaataat                                                         <  1:284863/140‑1 (MQ=255)                                                                       gTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCTCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTa                                                 <  1:172264/140‑1 (MQ=255)                                                                        tttCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAg                                                >  1:384805/1‑140 (MQ=255)                                                                         ttCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGt                                               >  1:487603/1‑140 (MQ=255)                                                                                  ataataTGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGt                                           >  1:557615/1‑135 (MQ=255)                                                                                  ataataTGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGt                                           <  2:557615/135‑1 (MQ=255)                                                                                           ccAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGc                             >  1:400510/1‑140 (MQ=255)                                                                                             aaCCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCg                           <  1:425485/140‑1 (MQ=255)                                                                                               ccTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCcaca                         >  1:136243/1‑140 (MQ=255)                                                                                               ccTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCa                         >  1:928160/1‑140 (MQ=255)                                                                                                 tctcATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAat                       <  2:928160/140‑1 (MQ=255)                                                                                                     aTAATTTAAATTTACCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATAtct                   <  1:930201/140‑1 (MQ=255)                                                                                                         tttaaatttaCCCCGCTCTGGTGATTCTCAAGCGCCAGATGATACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGAGGTGCCGCAATATCTCTGt               >  2:603189/1‑140 (MQ=255)                                                                                                                 taCCCCGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGAtt       <  2:384805/140‑1 (MQ=255)                                                                                                                   ccccGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTgg     >  2:534223/1‑140 (MQ=255)                                                                                                                     ccGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGa   <  1:160345/140‑1 (MQ=255)                                                                                                                      cGCTCTGGTGATTCTCAAGCGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGAt  <  2:400510/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                          CGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAGTGGGTATCAGTCGTGGTGCCGCAATATCTCTGTTCCCGATTGGGAT  >  NC_000913/3546241‑3546497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑