Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,707,324	C→T	A128T (GCG→ACG)	dppA ←	dipeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,707,324	0	C	T	100.0%	149.2 / NA	44	A128T (GCG→ACG)	dppA	dipeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/22);  total (22/22)

GGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGAATTCTTTATTGTCGTGCCACTTCACACCTTTACGCAGATGGAAGGTATAGGTTTTACCGTCTTCGCTGACTTCC  >  NC_000913/3707188‑3707460                                                                                                                                         |                                                                                                                                         ggCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGtt                                                                                                                                       <  1:18044/140‑1 (MQ=255)         cGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGAc                                                                                                                               <  1:929111/140‑1 (MQ=255)              gAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCg                                                                                                                          <  1:54960/140‑1 (MQ=255)                 tCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAAc                                                                                                                       >  1:270208/1‑140 (MQ=255)                       caAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAac                                                                                                                 <  1:9243/140‑1 (MQ=255)                          aCTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACga                                                                                                                     <  1:657747/133‑1 (MQ=255)                          aCTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACga                                                                                                                     >  2:657747/1‑133 (MQ=255)                              aaCGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCAcat                                                                                                          >  1:860827/1‑140 (MQ=255)                               aCGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCAcatc                                                                                                         >  2:562828/1‑140 (MQ=255)                                   tgttgtCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCg                                                                                                     <  1:626919/140‑1 (MQ=255)                                            ccACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCgttcagt                                                                                            >  2:583716/1‑140 (MQ=255)                                             cACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCgttcagtt                                                                                           >  1:711540/1‑140 (MQ=255)                                             cACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCgttca                                                                                              >  1:304189/1‑137 (MQ=255)                                             cACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCgttca                                                                                              <  2:304189/137‑1 (MQ=255)                                               ccTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCgttcagttca                                                                                         >  2:414386/1‑140 (MQ=255)                                                   tttCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCAcgcg                                                                                     <  1:414386/140‑1 (MQ=255)                                                     tcacttcactGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTc                                                                                   <  2:711540/140‑1 (MQ=255)                                                          tcactGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGttt                                                                              >  2:56725/1‑140 (MQ=255)                                                          tcactGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGttt                                                                              >  1:374683/1‑140 (MQ=255)                                                             ctGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATTGGCGTTCAGTTCACGCGTCGGTTTGaa                                                                           <  1:180781/140‑1 (MQ=255)                                                             ctGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGaa                                                                           <  2:44378/140‑1 (MQ=255)                                                                     ctctGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGAATTCTTTAt                                                                   >  2:448862/1‑140 (MQ=255)                                                                     ctctGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGAATTCTTTAt                                                                   >  1:442812/1‑140 (MQ=255)                                                                     ctctGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGAATTCTTTAt                                                                   >  2:721486/1‑140 (MQ=255)                                                                          gCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGAATTCTTTATTGTCg                                                              >  2:892879/1‑140 (MQ=255)                                                                          gCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGAATTCTTTATTGTCg                                                              >  1:595893/1‑140 (MQ=255)                                                                             aGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGAATTCTTTATTGTCGTGc                                                           <  1:892879/140‑1 (MQ=255)                                                                             aGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGAATTCTTTATTGTCGTGc                                                           <  2:442812/140‑1 (MQ=255)                                                                              gCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGAATTCTTTATTGTCGTGcc                                                          >  1:914195/1‑140 (MQ=255)                                                                               cccATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGaa                                                                           <  1:271086/122‑1 (MQ=255)                                                                               cccATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGaa                                                                           >  2:271086/1‑122 (MQ=255)                                                                               cccATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGAATTCTTTATTGTCGTGCCa                                                         >  1:547878/1‑140 (MQ=255)                                                                               cccATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGAATTCTTTATTGTCGTGCCa                                                         >  1:459722/1‑140 (MQ=255)                                                                               cccATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGAATTCTTTATTGTCGTGCCa                                                         >  2:329834/1‑140 (MQ=255)                                                                                    gCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGAATTCTTTATTGTCGTGCCACTTca                                                    <  1:329834/140‑1 (MQ=255)                                                                                    gCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTCCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGAATTCTTTATTGTCGTGCCACTTca                                                    <  2:860827/140‑1 (MQ=255)                                                                                     ccTTCGAAGTATTCGTAGCTACCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGAATTCTTTATTGTCGTGCCACTTcac                                                   <  2:476826/140‑1 (MQ=255)                                                                                      cTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGAATTCTTTATTGTCGTGCCACTTcaca                                                  <  1:448862/140‑1 (MQ=255)                                                                                              tATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGAATTCTTTATTGTCGTGCCACTTCACACCTTTACg                                          <  2:459722/140‑1 (MQ=255)                                                                                                  cGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGAATTCTTTATTGTCGTGCCACTTCACACCTTTACGCAGa                                      >  2:806977/1‑140 (MQ=255)                                                                                                    tAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGAATTCTTTATTGTCGTGCCACTTCACACCTTTACGCAGATg                                    <  1:806977/140‑1 (MQ=255)                                                                                                                       tATGGTACGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGAATTCTTTATTGTCGTGCCACTTCACACCTTTACGCAGATGGAAGGTATAGGTTTTACCg                 <  1:56725/140‑1 (MQ=255)                                                                                                                              cGGGTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGAATTCTTTATTGTCGTGCCACTTCACACCTTTACGCAGATGGAAGGTATAGGTTTTACCGTCTTCGc          <  2:547878/140‑1 (MQ=255)                                                                                                                               gggTTTTGCGTGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGAATTCTTTATTGTCGTGCCACTTCACACCTTTACGCAGATGGAAGGTATAGGTTTTACCGTCTTCGCt         >  1:562795/1‑140 (MQ=255)                                                                                                                                      gcgtGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGAATTCTTTATTGTCGTGCCACTTCACACCTTTACGCAGATGGAAGGTATAGGTTTTACCGTCTTCGCTGACTTcc  >  2:439927/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         GGCGCTTCCGGGCGAGTCAGCACAAACTGAACGGTGTTGTCGTCCACCTTTTTCACTTCACTGATCAGCTCTGGCAAGCCCATGCCTTCGAAGTATTCGTAGCTGCCGCCAGAAACTTTATGGTACGGGTTTTGCGCGTTTTTCTGACGATCGAACGAGAACACCACATCATCGGCGTTCAGTTCACGCGTCGGTTTGAATTCTTTATTGTCGTGCCACTTCACACCTTTACGCAGATGGAAGGTATAGGTTTTACCGTCTTCGCTGACTTCC  >  NC_000913/3707188‑3707460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑