Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,868,448 G→A H288Y (CAT→TAT)  yidR ← DUF3748 domain‑containing galacturonate catabolism protein YidR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,868,4480GA100.0% 147.9 / NA 43H288Y (CAT→TAT) yidRDUF3748 domain‑containing galacturonate catabolism protein YidR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/17);  total (26/17)

CGCCCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATCACCTGCCGCTTTCCAGCCAGCTTCATCTTGCGGTAACTCAACGATAAACAGC  >  NC_000913/3868316‑3868581
                                                                                                                                    |                                                                                                                                     
cgccCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTa                                                                                                                                <  1:49257/140‑1 (MQ=255)
           gagaTAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACg                                                                                                                     >  1:37569/1‑140 (MQ=255)
                                 tGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGGTAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGt                                                                                               >  2:414533/1‑140 (MQ=255)
                                 tGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGt                                                                                               >  1:210401/1‑140 (MQ=255)
                                 tGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGt                                                                                               >  1:392158/1‑140 (MQ=255)
                                   ccGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTgg                                                                                             <  2:705246/140‑1 (MQ=255)
                                    cGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGgc                                                                                            <  1:336257/140‑1 (MQ=255)
                                    cGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGgc                                                                                            <  2:37569/140‑1 (MQ=255)
                                          catcTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTACAGCGCAGCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGc                                                                                      <  2:210401/140‑1 (MQ=255)
                                             ctcGCATTAAAAACGCGATTTGTGTACCCTGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAgg                                                                                   >  2:623523/1‑140 (MQ=255)
                                               cGCATTAAAAACGCGATTTGTGTACCCTGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGgt                                                                                 <  1:414533/140‑1 (MQ=255)
                                                gCATTAAAAACGCGATTTGTGTACCCTGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGgtt                                                                                >  2:471483/1‑140 (MQ=255)
                                                  cattaaaaCGCGATTTGTGTACCCTGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGgttgt                                                                              >  1:810384/5‑140 (MQ=255)
                                                      aaaaCGCGATTTGTGTACCCTGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTttcc                                                                          >  2:323296/1‑140 (MQ=255)
                                                       aaaCGCGATTTGTGTACCCTGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTttccg                                                                         <  2:301991/140‑1 (MQ=255)
                                                         aCGCGATTTGTGTACCCTGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTttccgtt                                                                       <  1:535605/140‑1 (MQ=255)
                                                            cgATTTGTGTACCCTGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTttccgttccg                                                                    >  2:191111/1‑140 (MQ=255)
                                                                 tgtgTACCCTGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTaa                                                               <  1:668766/140‑1 (MQ=255)
                                                                  gtgtACCCTGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAAc                                                              <  2:392158/140‑1 (MQ=255)
                                                                          tGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAATGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATc                                                      >  2:765241/1‑140 (MQ=255)
                                                                          tGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATc                                                      >  2:837568/1‑140 (MQ=255)
                                                                          tGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATc                                                      >  2:288861/1‑140 (MQ=255)
                                                                          tGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATc                                                      >  1:298878/1‑140 (MQ=255)
                                                                          tGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATc                                                      >  2:372492/1‑140 (MQ=255)
                                                                            cGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATCAc                                                    >  2:400702/1‑140 (MQ=255)
                                                                             ggATTACAGCGCACCCAGTGGCNNGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTANAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATCAcc                                                   >  2:834201/1‑140 (MQ=255)
                                                                             ggATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATCAcc                                                   >  1:274263/1‑140 (MQ=255)
                                                                             ggATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATCAcc                                                   >  2:652116/1‑140 (MQ=255)
                                                                                      cgcACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATCACCTGCCGCttt                                          >  2:48182/1‑140 (MQ=255)
                                                                                      cgcACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATCACCTGCCGCttt                                          >  2:450153/1‑140 (MQ=255)
                                                                                              gTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATCACCTGCCGCTTTccagccag                                  <  1:765241/140‑1 (MQ=255)
                                                                                                     gggACGTTGACTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATCACCTGCCGCTTTCCAGCCAGCTTCATc                           >  2:333848/1‑140 (MQ=255)
                                                                                                              aCTAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATCACCTGCCGCTTTCCAGCCAGCTTCATCTTGCGGTaa                  <  1:450153/140‑1 (MQ=255)
                                                                                                                tAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATCACCTGCCGCTTTCCAGCCAGCTTCATCTTGCGGTAACt                <  1:415065/140‑1 (MQ=255)
                                                                                                                tAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATCACCTGCCGCTTTCCAGCCAGCTTCATCTTGCGGTAACt                <  2:298878/140‑1 (MQ=255)
                                                                                                                tAACCCCGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATCACCTGCCGCTTTCCAGCCAGCTTCATCTTGCGGTAACt                <  1:191111/140‑1 (MQ=255)
                                                                                                                   ccccGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATCACCTGCCGCTTTCCAGCCAGCTTCATCTTGCGGTAACTCaa             <  1:400702/140‑1 (MQ=255)
                                                                                                                   ccccGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATCACCTGCCGCTTTCCAGCCAGCTTCATCTTGCGGTAACTCaa             <  1:48182/140‑1 (MQ=255)
                                                                                                                     ccGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATCACCTGCCGCTTTCCAGCCAGCTTCATCTTGCGGTAACTCAACg           >  1:927061/1‑140 (MQ=255)
                                                                                                                      cGGATAAGCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATCACCTGCCGCTTTCCAGCCAGCTTCATCTTGCGGTAACTCAACGa          >  1:405341/1‑140 (MQ=255)
                                                                                                                             gCCCGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATCACCTGCCGCTTTCCAGCCAGCTTCATCTTGCGGTAACTCAACGATAAACAg   >  2:258683/1‑140 (MQ=255)
                                                                                                                              cccGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATCACCTGCCGCTTTCCAGCCAGCTTCATCTTGCGGTAACTCAACGATAAACAGc  >  1:514278/1‑140 (MQ=255)
                                                                                                                              cccGATAGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATCACCTGCCGCTTTCCAGCCAGCTTCATCTTGCGGTAACTCAACGATAAACAGc  >  1:748962/1‑140 (MQ=255)
                                                                                                                                    |                                                                                                                                     
CGCCCTGTGGCGAGATAAGCCACAGTTGCACAATGCCGTTATCATCTCGCATTAAAAACGCGATTTGTGTACCCTGCGGATTACAGCGCACCCAGTGGCGCGGGACGTTGACTAACCCCGGATAAGCCCGATGGTGGGTAAAGGTTAAACGTCGCTGCACGACGCCACGCGGTGGCGCGGGCAGGGTTGTTTCCGTTCCGCTTAACGGCGCATCACCTGCCGCTTTCCAGCCAGCTTCATCTTGCGGTAACTCAACGATAAACAGC  >  NC_000913/3868316‑3868581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑