Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	493,290	C→T	A400V (GCA→GTA)  A400V (GCA→GTA)	dnaX → dnaX →	DNA polymerase III subunit tau DNA polymerase III subunit tau

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	493,290	0	C	T	100.0%	144.1 / NA	43	A400V (GCA→GTA)  A400V (GCA→GTA)	dnaX dnaX	DNA polymerase III subunit tau DNA polymerase III subunit tau
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/24);  total (19/24)

CGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGC  >  NC_000913/493156‑493425                                                                                                                                       |                                                                                                                                        cgcgcTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGa                                                                                                                                    <  1:413823/140‑1 (MQ=255)      tGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTa                                                                                                                               >  2:355480/1‑140 (MQ=255)                  cgcgTATGCCGCTGCCTGAGCCAGTAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTACCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGaa                                                                                                                   >  1:477511/1‑140 (MQ=255)                       aTGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAaccac                                                                                                              <  2:238745/140‑1 (MQ=255)                             cTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCAccagcca                                                                                                        <  1:896591/140‑1 (MQ=255)                              tGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCAccagccag                                                                                                       >  2:207239/1‑140 (MQ=255)                                        aGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTggcgg                                                                                             <  2:559599/140‑1 (MQ=255)                                                cACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCcag                                                                                     <  1:355480/140‑1 (MQ=255)                                                        tCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCa                                                                             >  2:10713/1‑140 (MQ=255)                                                         ccTTTGCACCCGTTGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAg                                                                            >  1:86001/1‑140 (MQ=255)                                                         ccTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAg                                                                            <  2:587299/140‑1 (MQ=255)                                                             tGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCACAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGcgcg                                                                        >  2:830275/1‑140 (MQ=255)                                                                 cccGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCa                                                                    <  1:207239/140‑1 (MQ=255)                                                                  ccGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAg                                                                   >  1:290702/1‑140 (MQ=255)                                                                  ccGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAg                                                                   >  1:216641/1‑140 (MQ=255)                                                                    gTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAggg                                                                 >  1:278479/1‑140 (MQ=255)                                                                     tCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCACAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGa                                                                <  1:830275/140‑1 (MQ=255)                                                                      tgcgcCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAg                                                               <  2:86001/139‑1 (MQ=255)                                                                                 cAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCa                                                    <  2:52902/140‑1 (MQ=255)                                                                                 cAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCa                                                    <  2:94163/140‑1 (MQ=255)                                                                                   gTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCaaa                                                  <  2:216641/140‑1 (MQ=255)                                                                                     aaTGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCaaaaa                                                >  1:644614/1‑140 (MQ=255)                                                                                     aaTGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCaaaaa                                                <  2:290702/140‑1 (MQ=255)                                                                                        gACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAaga                                             <  1:771494/140‑1 (MQ=255)                                                                                         aCGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAagag                                            >  1:161174/1‑140 (MQ=255)                                                                                           gCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTg                                          >  1:558524/1‑140 (MQ=255)                                                                                                cccAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCg                                     >  1:652321/1‑140 (MQ=255)                                                                                                cccAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCg                                     >  1:292543/1‑140 (MQ=255)                                                                                                     gTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCaaaaa                                                <  2:199965/124‑1 (MQ=255)                                                                                                     gTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCaaaaa                                                >  1:199965/1‑124 (MQ=255)                                                                                                       gccgccGCAACCGCAATCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCg                              >  1:74829/1‑140 (MQ=255)                                                                                                           ccgcaaccgcaaTCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTAc                          >  1:217515/1‑140 (MQ=255)                                                                                                                  cgcaaTCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCcgcgcg                   <  2:292543/140‑1 (MQ=255)                                                                                                                     aaTCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCgg                <  1:134651/140‑1 (MQ=255)                                                                                                                      aTCAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGc               <  2:652321/140‑1 (MQ=255)                                                                                                                        cAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCg             <  1:336345/140‑1 (MQ=255)                                                                                                                        cAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCg             <  2:644614/140‑1 (MQ=255)                                                                                                                        cAGCGCCGCAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCg             <  2:161174/140‑1 (MQ=255)                                                                                                                           cgccgcAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGc               >  1:293982/1‑135 (MQ=255)                                                                                                                           cgccgcAGCAGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGc               <  2:293982/135‑1 (MQ=255)                                                                                                                               gcagcaGGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCTCGCGCGGCCGGTGAATa      >  2:901528/1‑140 (MQ=255)                                                                                                                                  gcagGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAAcg   <  2:278479/140‑1 (MQ=255)                                                                                                                                   cagGTACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGCGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAAcgc  <  1:619704/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        CGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAATCAGCGCCGCAGCAGGCACCGACTGTACCGCTCCCGGAAACCACCAGCCAGGTGCTGGCGGCGCGCCAGCAGTTGCAGCGCGTGCAGGGAGCAACCAAAGCAAAAAAGAGTGAACCGGCAGCCGCTACCCGCGCGCGGCCGGTGAATAACGC  >  NC_000913/493156‑493425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑