Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,954,853	A→G	D459G (GAC→GGC)	ilvD →	dihydroxy‑acid dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,954,853	0	A	G	100.0%	235.1 / NA	66	D459G (GAC→GGC)	ilvD	dihydroxy‑acid dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/37);  total (29/37)

CACGCCTACAGCAAAGACGGCGGCCTGGCGGTGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGACGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGAAGGCCCGAAAGGCGGTCCGGGGATGCAGGAAATGCTCTACCCAACCAGCTTCCTGAAATCAATGGGTC  >  NC_000913/3954717‑3954987                                                                                                                                         |                                                                                                                                       cacGCCTACAGCAAAGACGGCGGCCTGGCGGTGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGa                                                                                                                                     <  1:428300/140‑1 (MQ=255) cacGCCTACAGCAAAGACGGCGGCCTGGCGGTGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGa                                                                                                                                     <  2:257377/140‑1 (MQ=255)      cTACAGCAAAGACGGCGGCCTGGCGGTGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCgg                                                                                                                                >  2:105456/1‑140 (MQ=255)       tACAGCAAAGACGGCGGCCTGGCGGTGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGt                                                                                                                               <  1:541088/140‑1 (MQ=255)        aCAGCAAAGACGGCGGCCTGGCGGTGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTa                                                                                                                              <  2:558180/140‑1 (MQ=255)              aaGACGGCGGCCTGGCGGTGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGt                                                                                                                               >  2:680906/1‑133 (MQ=255)              aaGACGGCGGCCTGGAGGTGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGt                                                                                                                               <  1:680906/133‑1 (MQ=255)               aGACGGCGGCCTGGCGGTGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGa                                                                                                                       >  1:818160/1‑140 (MQ=255)                gACGGCGGCCTGGCGGTGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGAt                                                                                                                      >  2:353995/1‑140 (MQ=255)                    gcggcCTGGCGGTGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGt                                                                                                                               <  2:241091/127‑1 (MQ=255)                    gcggcCTGGCGGTGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGt                                                                                                                               >  1:241091/1‑127 (MQ=255)                    gcggcCTGGCGGTGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATtctc                                                                                                                  >  1:625316/1‑140 (MQ=255)                    gcggcCTGGCGGTGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCCGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATtctc                                                                                                                  >  1:376744/1‑140 (MQ=255)                      ggcCTGGCGGTGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATtct                                                                                                                   <  2:866774/137‑1 (MQ=255)                      ggcCTGGCGGTGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATtct                                                                                                                   >  1:866774/1‑137 (MQ=255)                      ggcCTGGCGGTGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTcgg                                                                                                                >  2:349890/1‑140 (MQ=255)                        ccTGGCGGTGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTcgg                                                                                                                >  2:906272/1‑138 (MQ=255)                        ccTGGCGGTGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTcgg                                                                                                                <  1:906272/138‑1 (MQ=255)                          tGGCGGTGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGt                                                                                                            <  1:235523/140‑1 (MQ=255)                             cGGTGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTaaa                                                                                                         >  1:544675/1‑140 (MQ=255)                                 gCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGGCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGGAGAAGCGATTCTCGGCGGGAAAAttt                                                                                                     >  2:579312/1‑139 (MQ=255)                                  ctctACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGt                                                                                                    <  2:215857/140‑1 (MQ=255)                                  ctctACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGt                                                                                                    <  1:664151/140‑1 (MQ=255)                                  ctctACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGt                                                                                                    <  1:105456/140‑1 (MQ=255)                                  ctctACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGt                                                                                                    <  1:349890/140‑1 (MQ=255)                                     tACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGc                                                                                                 <  2:504389/140‑1 (MQ=255)                                      aCGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGcc                                                                                                <  1:353995/140‑1 (MQ=255)                                         gTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGga                                                                                             <  1:429037/140‑1 (MQ=255)                                          tAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGgag                                                                                            >  2:518473/1‑140 (MQ=255)                                          tAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGgag                                                                                            >  1:204122/1‑140 (MQ=255)                                          tAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGGAAAGTTGTCGCCGgag                                                                                            >  2:480177/1‑140 (MQ=255)                                           aaCTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGgaga                                                                                           <  1:518473/140‑1 (MQ=255)                                            aCTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGAt                                                                                          >  2:685017/1‑140 (MQ=255)                                             cTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGAtg                                                                                         <  1:69899/140‑1 (MQ=255)                                                 gCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGgaga                                                                                           >  1:387286/1‑134 (MQ=255)                                                 gCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGgaga                                                                                           <  2:387286/134‑1 (MQ=255)                                                    gAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGga                                                                                             <  1:325943/129‑1 (MQ=255)                                                    gAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGga                                                                                             >  2:325943/1‑129 (MQ=255)                                                        aCGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAAtt                                                                              <  1:685017/140‑1 (MQ=255)                                                            cTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCt                                                                          >  1:774918/1‑140 (MQ=255)                                                               cATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATg                                                                       >  2:612248/1‑140 (MQ=255)                                                                 tCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGaa                                                                     <  2:625316/140‑1 (MQ=255)                                                                            gCAGGCGTCGATGACAGCATTCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATg                                                                       <  2:766607/127‑1 (MQ=255)                                                                            gCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATg                                                                       >  1:766607/1‑127 (MQ=255)                                                                            gCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGaa                                                                     <  2:909277/129‑1 (MQ=255)                                                                            gCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGaa                                                                     >  1:909277/1‑129 (MQ=255)                                                                            gCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGAAGGCCCGAAAgg                                                          <  2:376744/140‑1 (MQ=255)                                                                            gCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGAAGGCCCGAAAgg                                                          <  1:194796/140‑1 (MQ=255)                                                                              aGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGAAGGCCCGAAAgg                                                          >  1:82997/1‑138 (MQ=255)                                                                              aGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGAAGGCCCGAAAgg                                                          <  2:82997/138‑1 (MQ=255)                                                                                     gATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGAAGGCCCGAAAGGCg                                                        >  1:870048/1‑133 (MQ=255)                                                                                     gATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGAAGGCCCGAAAGGCg                                                        <  2:870048/133‑1 (MQ=255)                                                                                     gATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGAAGGCCCGAAAGGCGGTCCggg                                                 <  2:818160/140‑1 (MQ=255)                                                                                        gACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGAAGGCCCGAAAGGCGGTCCGGGGAt                                              <  2:544675/140‑1 (MQ=255)                                                                                         aCAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGAAGGCCCGAAAGGCGGTCCGGGGATg                                             <  1:579312/140‑1 (MQ=255)                                                                                              aTCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGAAGGCCCGAAAGGCGGTCCGGGGATGCAGGa                                        <  2:204122/140‑1 (MQ=255)                                                                                                    aaaTTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGAAGGCCCGAAAGGCGGTCCGGGGATGCAGGAAATGct                                  >  1:85832/1‑140 (MQ=255)                                                                                                         cACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGAAGGCCCGAAAGGCGGTCCGGGGATGCAGGAAATGct                                  >  2:246370/1‑135 (MQ=255)                                                                                                         cACCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGAAGGCCCGAAAGGCGGTCCGGGGATGCAGGAAATGct                                  <  1:246370/135‑1 (MQ=255)                                                                                                          aCCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGAAGGCCCGAAAGGCGGTCCGGGGATGCAGGAAATGCTCTAccc                            >  2:263902/1‑140 (MQ=255)                                                                                                          aCCGGCCCGGCGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGAAGGCCCGAAAGGCGGTCCGGGGATGCAGGAAATGCTCTAccc                            >  2:527295/1‑140 (MQ=255)                                                                                                           ccggcccggcGAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGAAGGCCCGAAAGGCGGTCCGGGGATGCAGGAAATGCTCTACCCa                           >  2:291721/1‑140 (MQ=255)                                                                                                                     gAAAGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGAAGGCCCGAAAGGCGGTCCGGGGATGCAGGAAATGCTCTACCCAACCAGCTTcc                 <  1:527295/140‑1 (MQ=255)                                                                                                                        aGTGTACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGAAGGCCCGAAAGGCGGTCCGGGGATGCAGGAAATGCTCTACCCAACCAGCTTCCTGa              <  1:263902/140‑1 (MQ=255)                                                                                                                         gtgtACGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGAAGGCCCGAAAGGCGGTCCGGGGATGCAGGAAATGCTCTACCCAACCAGCTTCCTGaa             <  1:612248/140‑1 (MQ=255)                                                                                                                              cGAAAGCCAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGAAGGCCCGAAAGGCGGTCCGGGGATGCAGGAAATGCTCTACCCAACCAGCTTCCTGAAATCaa        >  2:399159/1‑140 (MQ=255)                                                                                                                                    ccAGGGCGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGAAGGCCCGAAAGGCGGTCCGGGGATGCAGGAAATGCTCTACCCAACCAGCTTCCTGAAATCAATGGGtc  <  1:399159/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       CACGCCTACAGCAAAGACGGCGGCCTGGCGGTGCTCTACGGTAACTTTGCGGAAAACGGCTGCATCGTGAAAACGGCAGGCGTCGATGACAGCATCCTCAAATTCACCGGCCCGGCGAAAGTGTACGAAAGCCAGGACGATGCGGTAGAAGCGATTCTCGGCGGTAAAGTTGTCGCCGGAGATGTGGTAGTAATTCGCTATGAAGGCCCGAAAGGCGGTCCGGGGATGCAGGAAATGCTCTACCCAACCAGCTTCCTGAAATCAATGGGTC  >  NC_000913/3954717‑3954987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑