Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,277,869	A→G	N134S (AAC→AGC)	soxR →	DNA‑binding transcriptional dual regulator SoxR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,277,869	0	A	G	100.0%	134.1 / NA	38	N134S (AAC→AGC)	soxR	DNA‑binding transcriptional dual regulator SoxR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/17);  total (21/17)

GTGGAAACAGCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGG  >  NC_000913/4277738‑4278001                                                                                                                                    |                                                                                                                                     gTGGAAACAGCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCg                                                                                                                              <  1:907128/140‑1 (MQ=255)  tGGAAACAGCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGa                                                                                                                             <  1:574795/140‑1 (MQ=255)          gCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAg                                                                                                                     >  2:195949/1‑140 (MQ=255)             ttCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGgag                                                                                                                  <  1:633685/140‑1 (MQ=255)               cGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGagaa                                                                                                                <  2:52126/140‑1 (MQ=255)                              aagaGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGCCGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCa                                                                                                 <  1:384950/140‑1 (MQ=255)                                gagTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACg                                                                                               <  1:958308/140‑1 (MQ=255)                                           gcgcATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAg                                                                                    >  1:337839/1‑140 (MQ=255)                                           gcgcATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAg                                                                                    <  2:730095/140‑1 (MQ=255)                                                 tCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAAc                                                                              >  1:559542/1‑140 (MQ=255)                                                 tCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAAc                                                                              <  2:337839/140‑1 (MQ=255)                                                     aCCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACaaaa                                                                          >  1:631545/1‑140 (MQ=255)                                                          aGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTaaa                                                                     >  1:646430/1‑140 (MQ=255)                                                           gTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAg                                                                    >  2:390244/1‑140 (MQ=255)                                                               cgcTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAg                                                                    <  2:519990/136‑1 (MQ=255)                                                               cgcTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAg                                                                    >  1:519990/1‑136 (MQ=255)                                                                gcTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTa                                                                       <  2:713730/132‑1 (MQ=255)                                                                gcTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTa                                                                       >  1:713730/1‑132 (MQ=255)                                                                 cTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGTAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCcac                                                              >  2:481933/1‑140 (MQ=255)                                                                    cGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAg                                                           <  1:390244/140‑1 (MQ=255)                                                                                gACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAgtt                                               >  1:894938/1‑140 (MQ=255)                                                                                      tGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGtttt                                         >  2:767888/1‑140 (MQ=255)                                                                                       gTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTc                                        <  1:167123/140‑1 (MQ=255)                                                                                          ttGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCgg                                     >  1:34115/1‑140 (MQ=255)                                                                                             gTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtct                                  >  2:218998/1‑140 (MQ=255)                                                                                                 tgGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtct                                  >  1:4976/1‑136 (MQ=255)                                                                                                 tgGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtct                                  <  2:4976/136‑1 (MQ=255)                                                                                                  ggCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttgtc                             >  2:375129/1‑140 (MQ=255)                                                                                                    cTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttgtctt                           >  2:817968/1‑140 (MQ=255)                                                                                                       ccTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttg                               >  1:699469/1‑133 (MQ=255)                                                                                                       ccTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGgtctttg                               <  2:699469/133‑1 (MQ=255)                                                                                                       ccTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTtct                        >  2:904361/1‑140 (MQ=255)                                                                                                                cAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATccc               <  1:904361/140‑1 (MQ=255)                                                                                                                  gTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGc             <  2:34115/140‑1 (MQ=255)                                                                                                                             ttGCGTAGCCCGGGCGACCGCTTAGGATAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTg           <  2:7147/131‑1 (MQ=255)                                                                                                                             ttGCGTAGCCCGGGCGACCGCTTAGGATAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTg           >  1:7147/1‑131 (MQ=255)                                                                                                                             ttGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACagg  >  1:957287/1‑140 (MQ=255)                                                                                                                             ttGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTGTCCGGTCTTTGTCGTTCTCTCTATCCCGCTGGTAGACagg  >  2:851464/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                     GTGGAAACAGCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAGTTTGTTTTCCGGTCTTTGTCTTTCTCTCTATCCCGCTGGTACACAGG  >  NC_000913/4277738‑4278001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑