| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 494,207 | G→A | V44V (GTG→GTA) | ybaB → | putative nucleoid‑associated protein YbaB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 494,207 | 0 | G | A | 98.4% | 208.2 / NA | 61 | V44V (GTG→GTA) | ybaB | putative nucleoid‑associated protein YbaB |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (30/30); minor base T (0/1); total (30/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTGACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGACGCAGCACGTCGTATTGAAGAAACGCA > NC_000913/494070‑494338 | aaaCCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAAc > 1:244683/1‑140 (MQ=255)aaaCCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAAc > 1:554204/1‑140 (MQ=255) aTGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCaa > 1:603498/1‑140 (MQ=255) gTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACg < 2:884662/140‑1 (MQ=255) gTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATc > 1:752026/1‑131 (MQ=255) gTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATc > 1:668949/1‑131 (MQ=255) gTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATc < 2:752026/131‑1 (MQ=255) gTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATc < 2:668949/131‑1 (MQ=255) gTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGca < 2:244683/140‑1 (MQ=255) gTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGca > 2:55042/1‑140 (MQ=255) gTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGATGTCACCGGCGAATCTGGCGCAGGGCTGGTAAAAGTAACCATCAACGGTGCACACAACTGc > 2:861391/1‑140 (MQ=255) cTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCg < 2:306225/140‑1 (MQ=255) cTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCg < 1:861391/140‑1 (MQ=255) tAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTcgcg > 2:246451/1‑140 (MQ=255) cTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTaga > 2:592832/1‑140 (MQ=255) atgaAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGAt < 1:873642/140‑1 (MQ=255) tgaAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATc > 2:106655/1‑140 (MQ=255) tgaAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATc > 1:559372/1‑140 (MQ=255) tgaAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATc > 1:399261/1‑140 (MQ=255) gcaagcCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGAcc > 2:206943/1‑140 (MQ=255) gcaagcCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGAcc < 1:650473/140‑1 (MQ=255) caagcCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGAccc > 2:539239/1‑140 (MQ=255) caagcCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGAccc > 1:594359/1‑140 (MQ=255) ccAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGcc < 2:603498/140‑1 (MQ=255) agcagATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCctg > 1:169671/1‑140 (MQ=255) tGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGaa > 2:581479/1‑140 (MQ=255) gCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTTACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCctgc < 2:731830/134‑1 (MQ=255) gCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCctgc > 1:731830/1‑134 (MQ=255) gCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGaa > 1:878631/1‑139 (MQ=255) gCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGaa < 2:878631/139‑1 (MQ=255) aGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAgacg > 2:954388/1‑140 (MQ=255) aaatgcagaaaatgcagGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACaaa < 1:246451/140‑1 (MQ=255) aatgcagaaaatgcagGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAag < 1:581479/140‑1 (MQ=255) gcagaaaatgcagGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAgaga > 1:446979/1‑140 (MQ=255) tgcagGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGaa < 1:539239/140‑1 (MQ=255) agGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGAc < 2:559372/140‑1 (MQ=255) gAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCt < 2:417798/140‑1 (MQ=255) gAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCt < 2:255687/140‑1 (MQ=255) aaGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCtg < 1:212981/140‑1 (MQ=255) gaTCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCtggtgg < 2:446979/140‑1 (MQ=255) aTCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGc > 2:231427/1‑140 (MQ=255) aTCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGc > 2:305420/1‑140 (MQ=255) cgcAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTgca < 2:554204/140‑1 (MQ=255) cgcAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTgca > 2:45937/1‑140 (MQ=255) aGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTgcagca < 1:305420/140‑1 (MQ=255) gCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCtg > 1:624565/1‑127 (MQ=255) gCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCtg < 2:624565/127‑1 (MQ=255) gAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCaa < 1:61160/140‑1 (MQ=255) gAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCaa < 2:639427/140‑1 (MQ=255) gAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCaa < 1:231427/140‑1 (MQ=255) aGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAacg < 2:91961/140‑1 (MQ=255) cACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAacgacg < 1:45937/140‑1 (MQ=255) ccGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTgca > 1:824654/1‑125 (MQ=255) ccGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTgca < 2:824654/125‑1 (MQ=255) ccGGCGAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGACgca > 2:107113/1‑140 (MQ=255) gAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTgcagca > 1:948738/1‑123 (MQ=255) gAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTgcagca < 2:948738/123‑1 (MQ=255) gAATCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGACGCAGCAcg < 2:399261/140‑1 (MQ=255) aaTCTGGCGCAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGACGCAGCAcgt < 1:107113/140‑1 (MQ=255) cgcAGGTCTGGTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGACGCAGCACGTCGTATTg > 2:930144/1‑140 (MQ=255) gTAAAAGTAACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGACGCAGCACGTCGTATTGAAGAAACGCa > 2:710989/1‑140 (MQ=255) | AAACCTATGTTTGGTAAAGGCGGTCTGGGTAACCTGATGAAGCAAGCCCAGCAGATGCAAGAAAAAATGCAGAAAATGCAGGAAGAGATCGCGCAGCTGGAAGTCACCGGCGAATCTGGCGCAGGTCTGGTAAAAGTGACCATCAACGGTGCACACAACTGCCGTCGCGTAGAGATCGACCCGAGCCTGCTGGAAGACGACAAAGAGATGCTGGAAGACCTGGTGGCTGCAGCATTCAACGACGCAGCACGTCGTATTGAAGAAACGCA > NC_000913/494070‑494338 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |