Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,304,978	A→G	G540G (GGT→GGC)	yjcS ←	alkyl sulfatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,304,978	0	A	G	100.0%	209.4 / NA	59	G540G (GGT→GGC)	yjcS	alkyl sulfatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/30);  total (29/30)

GTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCG  >  NC_000913/4304846‑4305113                                                                                                                                     |                                                                                                                                        gTTGAGGCTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCCTTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCggtgg                                                                                                                                  >  2:734304/1‑140 (MQ=255)    gAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGc                                                                                                                               <  1:429140/140‑1 (MQ=255)     aGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGcc                                                                                                                              >  1:906933/1‑140 (MQ=255)         tATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTgg                                                                                                                          <  2:835335/140‑1 (MQ=255)          aTCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGc                                                                                                                         <  2:128737/140‑1 (MQ=255)           tCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTgg                                                                                                                          <  1:673426/138‑1 (MQ=255)           tCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTgg                                                                                                                          >  2:673426/1‑138 (MQ=255)           tCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGc                                                                                                                         <  1:245029/139‑1 (MQ=255)           tCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGc                                                                                                                         >  2:245029/1‑139 (MQ=255)            cGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTg                                                                                                                       <  2:906933/140‑1 (MQ=255)               cGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAAc                                                                                                                    >  2:674873/1‑140 (MQ=255)                   gCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTAt                                                                                                                <  1:273678/140‑1 (MQ=255)                    cTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATg                                                                                                               <  1:674873/140‑1 (MQ=255)                           tGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCt                                                                                                        <  1:316648/140‑1 (MQ=255)                           tGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCt                                                                                                        >  1:954720/1‑140 (MQ=255)                              aGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAAc                                                                                                                    <  2:775456/125‑1 (MQ=255)                              aGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAAc                                                                                                                    >  1:775456/1‑125 (MQ=255)                                 tCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCgcgc                                                                                                  >  2:449925/1‑140 (MQ=255)                                  cAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCa                                                                                                 >  2:717955/1‑140 (MQ=255)                                   aGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAg                                                                                                >  1:618635/1‑140 (MQ=255)                                         atatTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCttt                                                                                          >  2:395019/1‑140 (MQ=255)                                          tatTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTc                                                                                         >  1:172739/1‑140 (MQ=255)                                          tatTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTc                                                                                         >  1:37208/1‑140 (MQ=255)                                              tttACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCgcgc                                                                                     <  1:717955/140‑1 (MQ=255)                                              tttACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCgcgc                                                                                     <  2:80970/140‑1 (MQ=255)                                                tACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCg                                                                                   <  2:245946/140‑1 (MQ=255)                                                      cAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAgg                                                                             >  1:192460/1‑140 (MQ=255)                                                      cAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAgg                                                                             >  1:289246/1‑140 (MQ=255)                                                         cTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAg                                                                          >  2:42977/1‑140 (MQ=255)                                                                 cTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACcgcg                                                                  <  2:192460/140‑1 (MQ=255)                                                                   aTCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGcc                                                                <  1:754890/140‑1 (MQ=255)                                                                         gCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTgg                                                          >  1:716671/1‑140 (MQ=255)                                                                            aaCGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCgg                                                       >  2:198367/1‑140 (MQ=255)                                                                              cGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGAc                                                     >  2:654893/1‑140 (MQ=255)                                                                              cGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGAc                                                     >  1:18659/1‑140 (MQ=255)                                                                              cGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGAc                                                     >  1:93934/1‑140 (MQ=255)                                                                               ggCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACt                                                    <  1:395019/140‑1 (MQ=255)                                                                                            aaCAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGcc                                       >  1:901433/1‑140 (MQ=255)                                                                                              cAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCCCCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCa                                     <  2:69895/140‑1 (MQ=255)                                                                                               aGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAg                                    <  2:289246/140‑1 (MQ=255)                                                                                               aGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAg                                    <  1:654893/140‑1 (MQ=255)                                                                                                 cATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCt                                  <  2:18659/140‑1 (MQ=255)                                                                                                 cATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCt                                  <  1:523398/140‑1 (MQ=255)                                                                                                 cATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCt                                  >  1:651433/1‑140 (MQ=255)                                                                                                             cATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAg                                    <  1:513381/126‑1 (MQ=255)                                                                                                             cATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAg                                    >  2:513381/1‑126 (MQ=255)                                                                                                             cATCCCGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTAt                      >  2:933920/1‑140 (MQ=255)                                                                                                                cccGCGAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCg                   <  2:954720/140‑1 (MQ=255)                                                                                                                   gcgAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGt                                <  2:258955/124‑1 (MQ=255)                                                                                                                   gcgAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGt                                >  1:258955/1‑124 (MQ=255)                                                                                                                    cgAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTg               <  2:716671/140‑1 (MQ=255)                                                                                                                     gAATGGTGTCCGGGGAGCCGGTGGTGCCGTGGCTTAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGc              >  2:717694/1‑140 (MQ=255)                                                                                                                           tgtCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTa                       <  2:50980/125‑1 (MQ=255)                                                                                                                           tgtCCGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTa                       >  1:50980/1‑125 (MQ=255)                                                                                                                              ccGGGGAGCCGGTGGTGCCGTGGCTGATCTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAACCCGCGCCATGTGGCGGTCTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttcttc     <  1:734304/140‑1 (MQ=255)                                                                                                                              ccGGGGAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGGAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGAttcttc     <  2:618635/140‑1 (MQ=255)                                                                                                                                 gggAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTcgcg  >  2:506427/1‑140 (MQ=255)                                                                                                                                 gggAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTcgcg  >  1:146435/1‑140 (MQ=255)                                                                                                                                 gggAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTcgcg  <  1:449925/140‑1 (MQ=255)                                                                                                                                 gggAGCCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTcgcg  <  2:37208/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        GTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGCTCTTTCGCGCCGGTCAGGTAGAAACCGCGCCATGTGGCGGACTCGGCCTGATAGCCCAGCTGTTCAAAGTTATTCGCTTGCAGATTCTTCGCG  >  NC_000913/4304846‑4305113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑