Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,316,944	A→G	V92A (GTA→GCA)	phnM ←	RPnTP hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,316,944	0	A	G	100.0%	196.1 / NA	55	V92A (GTA→GCA)	phnM	RPnTP hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/33);  total (22/33)

CGCAGCGCAGATGCAGACGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGC  >  NC_000913/4316807‑4317078                                                                                                                                          |                                                                                                                                       cgcagcgcagATGCAGACGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCg                                                                                                                                      >  2:722610/1‑140 (MQ=255) cgcagcgcagATGCAGACGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCg                                                                                                                                      <  2:697311/140‑1 (MQ=255)        cagATGCAGACGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGa                                                                                                                               <  2:817931/140‑1 (MQ=255)        cagATGCAGACGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGa                                                                                                                               <  2:308326/140‑1 (MQ=255)              cAGACGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGc                                                                                                                         <  2:822538/140‑1 (MQ=255)                gACGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTc                                                                                                                       <  2:279173/140‑1 (MQ=255)                  cGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGc                                                                                                                     <  2:173213/140‑1 (MQ=255)                  cGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGc                                                                                                                     <  1:319881/140‑1 (MQ=255)                     tGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGccac                                                                                                                  >  1:921779/1‑140 (MQ=255)                     tGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGccac                                                                                                                  >  1:78498/1‑140 (MQ=255)                     tGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGccac                                                                                                                  >  1:181944/1‑140 (MQ=255)                       cTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCTGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGccacca                                                                                                                >  2:69585/1‑140 (MQ=255)                       cTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGc                                                                                                                         <  1:41676/131‑1 (MQ=255)                       cTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGc                                                                                                                         >  2:41676/1‑131 (MQ=255)                           gcgcGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAg                                                                                                            <  2:402416/140‑1 (MQ=255)                                gTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAACCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGcgt                                                                                                       <  2:864841/140‑1 (MQ=255)                                  tGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGcgtcg                                                                                                     >  2:555723/1‑140 (MQ=255)                                     cgccgcGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTgg                                                                                                  <  2:285017/140‑1 (MQ=255)                                      gccgcGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCTGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGgc                                                                                                 <  1:69585/140‑1 (MQ=255)                                         gcgTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGgctgc                                                                                              <  1:863211/140‑1 (MQ=255)                                           gTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTc                                                                                            >  2:811848/1‑140 (MQ=255)                                                   gTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGa                                                                                    <  1:67327/140‑1 (MQ=255)                                                   gTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGa                                                                                    <  1:811848/140‑1 (MQ=255)                                                      tctTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGgctgc                                                                                              >  2:483150/1‑127 (MQ=255)                                                      tctTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGgctgc                                                                                              <  1:483150/127‑1 (MQ=255)                                                       ctTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATc                                                                                         <  1:609350/131‑1 (MQ=255)                                                       ctTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATc                                                                                         >  2:609350/1‑131 (MQ=255)                                                       ctTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTgg                                                                                >  2:161060/1‑140 (MQ=255)                                                       ctTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTgg                                                                                >  1:86097/1‑140 (MQ=255)                                                       ctTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTgg                                                                                >  1:298315/1‑140 (MQ=255)                                                          cGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCa                                                                             <  2:921779/140‑1 (MQ=255)                                                              ggCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGcc                                                                         >  1:628769/1‑140 (MQ=255)                                                                 gTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGt                                                                      <  2:78498/140‑1 (MQ=255)                                                                 gTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGt                                                                      <  2:86097/140‑1 (MQ=255)                                                                 gTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGt                                                                      <  2:590923/140‑1 (MQ=255)                                                                         tcttctCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTc                                                              >  1:234579/1‑140 (MQ=255)                                                                         tcttctCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTc                                                              >  1:208713/1‑140 (MQ=255)                                                                             ctcCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGgc                                                          >  1:151202/1‑140 (MQ=255)                                                                              tcCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGgcg                                                         <  2:181944/140‑1 (MQ=255)                                                                               ccAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGgcgc                                                        >  2:307216/1‑140 (MQ=255)                                                                                   aTTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCgggg                                                    >  2:589042/1‑140 (MQ=255)                                                                                     tctcCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTg                                                  <  2:571722/140‑1 (MQ=255)                                                                                      ctcCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTga                                                 <  2:234579/140‑1 (MQ=255)                                                                                       tcCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTgaa                                                >  2:481550/1‑140 (MQ=255)                                                                                            ccGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAAtt                                           >  1:174685/1‑140 (MQ=255)                                                                                                     gccgTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGAt                                  <  1:845831/140‑1 (MQ=255)                                                                                                     gccgTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGAt                                  <  1:722610/140‑1 (MQ=255)                                                                                                        gTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTAt                               <  2:208713/140‑1 (MQ=255)                                                                                                                  tCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAg                     <  2:174685/140‑1 (MQ=255)                                                                                                                  tCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAg                     <  1:307216/140‑1 (MQ=255)                                                                                                                   cGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGc                    >  1:204424/1‑140 (MQ=255)                                                                                                                      cAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCa                 <  1:555723/140‑1 (MQ=255)                                                                                                                       aaTTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCaa                <  2:38099/140‑1 (MQ=255)                                                                                                                                  cATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGgc     <  2:204424/140‑1 (MQ=255)                                                                                                                                     ccAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGgcagc  <  1:589042/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       CGCAGCGCAGATGCAGACGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGC  >  NC_000913/4316807‑4317078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑