Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,445,5490AG100.0% 218.9 / NA 62T480A (ACC→GCC) tamBtranslocation and assembly module subunit TamB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/37);  total (25/37)

CTGGCAACAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCAAA  >  NC_000913/4445422‑4445685
                                                                                                                               |                                                                                                                                        
cTGGCAACAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATgg                                                                                                                              <  1:203824/140‑1 (MQ=255)
 tGGCAACAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGa                                                                                                                             <  1:112836/140‑1 (MQ=255)
   gCAACAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACg                                                                                                                           >  1:958348/1‑140 (MQ=255)
     aaCAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTg                                                                                                                         >  1:258831/1‑140 (MQ=255)
          gCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGa                                                                                                                             >  1:346989/1‑131 (MQ=255)
          gCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGa                                                                                                                             <  2:346989/131‑1 (MQ=255)
          gCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGa                                                                                                                    >  2:395679/1‑140 (MQ=255)
          gCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGa                                                                                                                    >  2:454800/1‑140 (MQ=255)
             aTTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGtt                                                                                                                 >  1:834718/1‑140 (MQ=255)
                   tGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCt                                                                                                           <  2:35425/140‑1 (MQ=255)
                   tGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCt                                                                                                           <  2:190401/140‑1 (MQ=255)
                    ggCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTg                                                                                                          >  1:902989/1‑140 (MQ=255)
                    ggCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTg                                                                                                          >  1:858144/1‑140 (MQ=255)
                      cgcgGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGAc                                                                                                        >  1:614059/1‑140 (MQ=255)
                         ggTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAATACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCgg                                                                                                     <  1:423755/140‑1 (MQ=255)
                         ggTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCgg                                                                                                     <  2:958348/140‑1 (MQ=255)
                         ggTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCgg                                                                                                     <  2:834718/140‑1 (MQ=255)
                           tGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTa                                                                                                   >  2:175502/1‑140 (MQ=255)
                              gCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACg                                                                                                <  1:402761/140‑1 (MQ=255)
                                       tAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGCTGAAGCTGACCGGTAACg                                                                                                <  2:756158/131‑1 (MQ=255)
                                       tAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGCTGAAGCTGACCGGTAACg                                                                                                >  1:756158/1‑131 (MQ=255)
                                            gCATTAACACCGCCAAAGAGTTCCCGGACTGACCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACaaa                                                                                  >  1:672351/1‑140 (MQ=255)
                                             cATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAg                                                                                 <  2:858144/140‑1 (MQ=255)
                                                     ccGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGtt                                                                         <  1:4104/140‑1 (MQ=255)
                                                       gCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTGACGTTAAACAGAACAAAGTGAACGTTGa                                                                       >  2:152616/1‑140 (MQ=255)
                                                       gCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGa                                                                       >  2:274481/1‑140 (MQ=255)
                                                       gCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGa                                                                       >  2:195709/1‑140 (MQ=255)
                                                           aaGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGc                                                                   >  1:105974/1‑140 (MQ=255)
                                                            agagTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCa                                                                  <  2:902989/140‑1 (MQ=255)
                                                            agagTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCa                                                                  <  2:420312/140‑1 (MQ=255)
                                                              agTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACg                                                                >  1:650809/1‑140 (MQ=255)
                                                                ttCCCGGACTGG‑CGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTg                                                             >  2:267508/1‑140 (MQ=255)
                                                                     ggACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAg                                                         >  2:604928/1‑140 (MQ=255)
                                                                     ggACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGCAACGTTAAACAGAACAAAGAGAACGCTGACGGCACGCTGAAAg                                                         >  2:952899/1‑140 (MQ=255)
                                                                       aCTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGc                                                       <  2:490363/140‑1 (MQ=255)
                                                                         tGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                     <  2:105166/140‑1 (MQ=255)
                                                                         tGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                     <  1:714302/140‑1 (MQ=255)
                                                                         tGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                     <  1:395679/140‑1 (MQ=255)
                                                                         gggCCGTCGAAACTCAATGGCTTGATTAAATCCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCaa                                                     <  1:952899/139‑1 (MQ=255)
                                                                           gCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGc                                                       <  1:147290/136‑1 (MQ=255)
                                                                           gCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGc                                                       >  2:147290/1‑136 (MQ=255)
                                                                              gTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGtt                                                <  2:650809/140‑1 (MQ=255)
                                                                              gTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTAAAACAGAACAAAGTGAACCTTGACGGCACGCTGAAAGGCAACAGtt                                                <  2:848090/140‑1 (MQ=255)
                                                                                cGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTtat                                              <  2:614059/140‑1 (MQ=255)
                                                                                 gAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTtata                                             <  1:175502/140‑1 (MQ=255)
                                                                                      tCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAg                                        <  1:955687/140‑1 (MQ=255)
                                                                                      tCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAg                                        <  1:402180/140‑1 (MQ=255)
                                                                                          tGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGga                                    >  1:203153/1‑140 (MQ=255)
                                                                                          tGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGga                                    <  2:203153/140‑1 (MQ=255)
                                                                                           ggCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGgat                                   <  1:267508/140‑1 (MQ=255)
                                                                                             cTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGgatga                                 <  2:29073/140‑1 (MQ=255)
                                                                                                      aaCCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAggg                         <  1:775870/139‑1 (MQ=255)
                                                                                                      aaCCCGCGGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAggg                         >  2:775870/1‑139 (MQ=255)
                                                                                                            cgGTAGCCTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATc                  <  2:232811/140‑1 (MQ=255)
                                                                                                                  ccTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAAc            <  2:258831/140‑1 (MQ=255)
                                                                                                                  ccTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAAc            <  1:454800/140‑1 (MQ=255)
                                                                                                                  ccTGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAAc            <  2:105974/140‑1 (MQ=255)
                                                                                                                    tGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATc                  >  2:495243/1‑132 (MQ=255)
                                                                                                                    tGTACGGCGGCGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATc                  <  1:495243/132‑1 (MQ=255)
                                                                                                                           cggcGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAAc            >  2:730681/1‑131 (MQ=255)
                                                                                                                           cggcGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAAc            <  1:730681/131‑1 (MQ=255)
                                                                                                                            ggcGCCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCaaa  <  2:869831/140‑1 (MQ=255)
                                                                                                                               |                                                                                                                                        
CTGGCAACAGGCCATTAGTTGGCGCGGTGAGCTAACGCTTAACGGCATTAACACCGCCAAAGAGTTCCCGGACTGGCCGTCGAAACTCAATGGCTTGATTAAAACCCGCGGTAGCCTGTACGGCGGCACCTGGCAGATGGACGTGCCGGAGTTGAAGCTGACCGGTAACGTTAAACAGAACAAAGTGAACGTTGACGGCACGCTGAAAGGCAACAGTTATATGCAGTGGATGATCCCAGGGCTTCATCTGGAACTCGGGCCAAA  >  NC_000913/4445422‑4445685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑