Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,596,841	C→T	S284S (AGC→AGT)	lgoD →	L‑galactonate oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,596,841	0	C	T	100.0%	154.6 / NA	45	S284S (AGC→AGT)	lgoD	L‑galactonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/22);  total (23/22)

GTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTACGAGCGCGATGTGATTAA  >  NC_000913/4596705‑4596975                                                                                                                                         |                                                                                                                                       gTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGc                                                                                                                                     <  1:934225/140‑1 (MQ=255)        aCATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAacgcca                                                                                                                              >  2:674344/1‑140 (MQ=255)        aCATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAacgcca                                                                                                                              <  2:653928/140‑1 (MQ=255)           tGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAacgccacgc                                                                                                                           >  2:164665/1‑140 (MQ=255)           tGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAacgccacgc                                                                                                                           >  1:51017/1‑140 (MQ=255)           tGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAacgccacgc                                                                                                                           >  1:912135/1‑140 (MQ=255)              gATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCgg                                                                                                                        <  2:594318/140‑1 (MQ=255)                   aTAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGAt                                                                                                                   >  2:85942/1‑140 (MQ=255)                    tAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGAtt                                                                                                                  >  2:608515/1‑140 (MQ=255)                          cGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTa                                                                                                            >  2:45250/1‑140 (MQ=255)                               aTTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAgtc                                                                                                       >  1:575028/1‑140 (MQ=255)                                tttGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAgtcg                                                                                                      <  1:164665/140‑1 (MQ=255)                                 ttGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAgtcgg                                                                                                     <  1:14421/140‑1 (MQ=255)                                  tGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAgtcggt                                                                                                    <  2:928372/140‑1 (MQ=255)                                  tGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAgtcggt                                                                                                    <  2:51017/140‑1 (MQ=255)                                                                 cGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAAt                                                                             >  2:844615/1‑132 (MQ=255)                                                                 cGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAAt                                                                             <  1:844615/132‑1 (MQ=255)                                                                 cGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCActgctg                                                                     >  2:120585/1‑140 (MQ=255)                                                                   gCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGCTGGCGGAAGGAAAAATCACTGCTGAc                                                                   >  1:99507/1‑140 (MQ=255)                                                                   gCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGAc                                                                   >  1:560856/1‑140 (MQ=255)                                                                    ccTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACa                                                                  <  1:85942/140‑1 (MQ=255)                                                                     cTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACat                                                                 <  1:674344/140‑1 (MQ=255)                                                                        tttAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACa                                                                  >  2:300684/1‑136 (MQ=255)                                                                        tttAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACa                                                                  <  1:300684/136‑1 (MQ=255)                                                                               gTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAAcc                                                       <  1:45250/140‑1 (MQ=255)                                                                                  aGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACa                                                                  <  2:130143/126‑1 (MQ=255)                                                                                  aGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACa                                                                  >  1:130143/1‑126 (MQ=255)                                                                                  aGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAAc                                                        <  1:501734/136‑1 (MQ=255)                                                                                  aGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAAc                                                        >  2:501734/1‑136 (MQ=255)                                                                                  aGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCAt                                                    >  1:829212/1‑140 (MQ=255)                                                                                  aGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCAt                                                    >  1:285695/1‑140 (MQ=255)                                                                                  aGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCAt                                                    >  1:441691/1‑140 (MQ=255)                                                                                  aGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCAt                                                    >  2:299424/1‑140 (MQ=255)                                                                                      gCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCt                                                >  2:624808/1‑140 (MQ=255)                                                                                      gCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCt                                                <  2:135401/140‑1 (MQ=255)                                                                                                gATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGc                                      <  1:608515/140‑1 (MQ=255)                                                                                                     ggAATTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGc                                 <  1:299424/140‑1 (MQ=255)                                                                                                        aTTCCATAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTgg                              <  1:624808/140‑1 (MQ=255)                                                                                                              tAAAAAAGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGaaa                        >  1:476344/1‑140 (MQ=255)                                                                                                                aaaaaGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAAcc                      >  2:374723/1‑140 (MQ=255)                                                                                                                  aaaGAAACGACGATGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTa                    <  2:329865/140‑1 (MQ=255)                                                                                                                            cgaTGATGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTACGAGCGCGAt          <  1:637593/140‑1 (MQ=255)                                                                                                                             gatgatGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTACGAGcgcg            >  1:708036/1‑137 (MQ=255)                                                                                                                             gatgatGGGCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTACGAGcgcg            <  2:708036/137‑1 (MQ=255)                                                                                                                                    ggCAGTCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTACGAGCGCGATGTGATTaa  <  2:829212/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       GTAATCAACATGCGATGAATAACACCGTGAATTTGATTCGTCACGGCGGCACGGTGGTATTTGTCGGCCTGTTTAAAGGTGAGTTGCAGTTCTCCGATCCGGAATTCCATAAAAAAGAAACGACGATGATGGGCAGCCGCAACGCCACGCCGGAAGATTTTGCTAAAGTCGGTCGACTGATGGCGGAAGGAAAAATCACTGCTGACATGATGTTAACCCATCGCTATCCGTTCGCCACGCTGGCAGAAACCTACGAGCGCGATGTGATTAA  >  NC_000913/4596705‑4596975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑