Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 89,416 A→G intergenic (+384/‑218) cra → / → mraZ DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091389,4160AG100.0% 199.1 / NA 55intergenic (+384/‑218)cra/mraZDNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/28);  total (27/28)

TTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGTAACAAGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTT  >  NC_000913/89279‑89543
                                                                                                                                         |                                                                                                                               
ttGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGTAACAAGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCg                                                                                                                               <  2:208472/140‑1 (MQ=255)
         cccGCTGGCATCAAGCGCCACACAGACGTAACAAGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACg                                                                                                                      <  1:121519/140‑1 (MQ=255)
         cccGCTGGCATCAAGCGCCACACAGACGTAACAAGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACg                                                                                                                      <  1:775467/140‑1 (MQ=255)
             cTGGCATCAAGCGCCACACAGACGTAACAAGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGa                                                                                                                  >  2:128026/1‑140 (MQ=255)
                         gcCACACAGACGTAACAAGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCt                                                                                                      <  1:121481/140‑1 (MQ=255)
                         gcCACACAGACGTAACAAGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCt                                                                                                      <  1:243652/140‑1 (MQ=255)
                                  aCGTAACAAGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTAt                                                                                             >  1:178972/1‑140 (MQ=255)
                                     tAACAAGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGc                                                                                                <  2:690328/134‑1 (MQ=255)
                                     tAACAAGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGc                                                                                                >  1:690328/1‑134 (MQ=255)
                                     tAACAAGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCa                                                                                          >  2:187070/1‑140 (MQ=255)
                                       aCAAGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCt                                                                                                      <  2:601736/126‑1 (MQ=255)
                                       aCAAGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCt                                                                                                      >  1:601736/1‑126 (MQ=255)
                                            gACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGtt                                                                                   >  1:435964/1‑140 (MQ=255)
                                            gACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGtt                                                                                   >  2:641674/1‑140 (MQ=255)
                                              cTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTaa                                                                                 >  1:399485/1‑140 (MQ=255)
                                                gTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAAtt                                                                               <  2:171038/140‑1 (MQ=255)
                                                   aaCCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGc                                                                            >  1:395092/1‑140 (MQ=255)
                                                                    ccTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTa                                                           <  1:187070/140‑1 (MQ=255)
                                                                     cTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTaa                                                          >  2:568724/1‑140 (MQ=255)
                                                                        aaaTGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAAt                                                       >  1:507560/1‑140 (MQ=255)
                                                                        aaaTGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAAt                                                       >  1:822447/1‑140 (MQ=255)
                                                                            gCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAaac                                                   <  2:674196/140‑1 (MQ=255)
                                                                            gCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAaac                                                   <  1:783449/140‑1 (MQ=255)
                                                                             ccGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAaaca                                                  >  2:896702/1‑140 (MQ=255)
                                                                             ccGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAaac                                                   <  2:419466/139‑1 (MQ=255)
                                                                             ccGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAaac                                                   >  1:419466/1‑139 (MQ=255)
                                                                              cGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAaacaa                                                 <  1:879535/140‑1 (MQ=255)
                                                                              cGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAaacaa                                                 <  2:178972/140‑1 (MQ=255)
                                                                                cTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATaaa                                                    <  2:962533/135‑1 (MQ=255)
                                                                                cTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATaaa                                                    >  1:962533/1‑135 (MQ=255)
                                                                                cTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACg                                               >  1:346112/1‑140 (MQ=255)
                                                                                     gTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGat                                          >  2:751908/1‑140 (MQ=255)
                                                                                       cGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGatat                                        <  2:768781/140‑1 (MQ=255)
                                                                                                    tttATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACAt                           >  2:23114/1‑140 (MQ=255)
                                                                                                      tataTTTGCTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCg                         >  1:881818/1‑140 (MQ=255)
                                                                                                              cTGTGGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGtttttt                 >  1:197201/1‑140 (MQ=255)
                                                                                                               tgtgGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTa                <  1:128026/140‑1 (MQ=255)
                                                                                                                gtgGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTAc               >  2:91575/1‑140 (MQ=255)
                                                                                                                gtgGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTAc               <  2:507560/140‑1 (MQ=255)
                                                                                                                gtgGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTAc               <  1:641674/140‑1 (MQ=255)
                                                                                                                gtgGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTAc               >  1:828669/1‑140 (MQ=255)
                                                                                                                gtgGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTAc               <  2:881818/140‑1 (MQ=255)
                                                                                                                gtgGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTAc               <  2:822447/140‑1 (MQ=255)
                                                                                                                 tgGAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTAcc              <  2:828669/140‑1 (MQ=255)
                                                                                                                  ggAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTAcc              <  1:697289/139‑1 (MQ=255)
                                                                                                                  ggAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTAcc              >  2:697289/1‑139 (MQ=255)
                                                                                                                  ggAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCt             >  2:453833/1‑140 (MQ=255)
                                                                                                                   gAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTc            <  2:119549/140‑1 (MQ=255)
                                                                                                                   gAAAATAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTc            <  1:568724/140‑1 (MQ=255)
                                                                                                                      aaTAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGt         <  2:395092/140‑1 (MQ=255)
                                                                                                                      aaTAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGt         <  1:23114/140‑1 (MQ=255)
                                                                                                                       aTAGTGAGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTa        <  2:399485/140‑1 (MQ=255)
                                                                                                                             aGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCtt  >  1:502899/1‑140 (MQ=255)
                                                                                                                             aGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCtt  >  2:48651/1‑140 (MQ=255)
                                                                                                                             aGTCATTTTAAAGCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCtt  >  2:474120/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                               
TTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGTAACAAGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTT  >  NC_000913/89279‑89543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑