Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	589,810	A→G	L382P (CTG→CCG)	nfrA ←	bacteriophage N4 receptor, outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	589,810	0	A	G	100.0%	165.3 / NA	46	L382P (CTG→CCG)	nfrA	bacteriophage N4 receptor, outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/24);  total (22/24)

TCCAGCAGAGACGCCAGTCGCGCCATTAAAGTCTGGCTGACACGCGCATCGCCCTGGAAAGGATAGCGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCA  >  NC_000913/589675‑589942                                                                                                                                        |                                                                                                                                     tCCAGCAGAGACGCCAGTCGCGCCATTAAAGTCTGGCTGACACGCGCATCGCCCTGGAAAGGATAGCGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCg                                                                                                                                  >  2:9026/1‑140 (MQ=255) tCCAGCAGAGACGCCAGTCGCGCCATTAAAGTCTGGCTGACACGCGCATCGCCCTGGAAAGGATAGCGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCg                                                                                                                                  >  1:174136/1‑140 (MQ=255)   cagcagAGACGCCAGTCGCGCCATTAAAGTCTGGCTGACACGCGCATCGCCCTGGAAAGGATAGCGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCcgggcggg                                                                                                                                >  2:507917/1‑140 (MQ=255)         agaCGCCAGTCGCGCCATTAAAGTCTGGCTGACACGCGCATCGCCCTGGAAAGGATAGCGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGAt                                                                                                                          >  2:79406/1‑140 (MQ=255)           aCGCCAGTCGCGCCATTAAAGTCTGGCTGACACGCGCATCGCCCTGGAAAGGATAGCGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGAttt                                                                                                                        >  2:164823/1‑140 (MQ=255)              ccAGTCGCGCCATTAAAGTCTGGCTGACACGCGCATCGCCCTGGAAAGGATAGCGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGcc                                                                                                                     <  2:174136/140‑1 (MQ=255)                  tCGCGCCATTAAAGTCTGGCTGACACGCGCATCGCCCTGGAAAGGATAGCGTTGCAGCAATAAATCGGCAGCTTCGCGTGCCTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGtt                                                                                                                 <  2:665472/140‑1 (MQ=255)                  tCGCGCCATTAAAGTCTGGCTGACACGCGCATCGCCCTGGAAAGGATAGCGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGtt                                                                                                                 <  2:167819/140‑1 (MQ=255)                  tCGCGCCATTAAAGTCTGGCTGACACGCGCATCGCCCTGGAAAGGATAGCGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGtt                                                                                                                 <  2:516775/140‑1 (MQ=255)                   cgcgCCATTAAAGTCTGGCTGACACGCGCATCGCCCCGGAAAGGATAGCGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTc                                                                                                                >  2:799572/1‑140 (MQ=255)                    gcgcCATTAAAGTCTGGCTGACACGCGCATCGCCCTGGAAAGGATAGCGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCt                                                                                                               <  1:164823/140‑1 (MQ=255)                        cATTAAAGTCTGGCTGACACGCGCATCGCCCTGGAAAGGATAGCGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCt                                                                                                           <  1:79406/140‑1 (MQ=255)                             aaGTCTGGCTGACACGCGCATCGCCCTGGAAAGGATAGCGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATAc                                                                                                      <  1:698463/140‑1 (MQ=255)                               gTCTGGCTGACACGCGCATCGCCCTGGAAAGGATAGCGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAg                                                                                                    <  1:621248/140‑1 (MQ=255)                                    gCTGACACGCGCATCGCCCTGGAAAGGATAGCGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTg                                                                                                          <  1:162417/129‑1 (MQ=255)                                    gCTGACACGCGCATCGCCCTGGAAAGGATAGCGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTg                                                                                                          >  2:162417/1‑129 (MQ=255)                                    gCTGACACGCGCATCGCCCTGGAAAGGATAGCGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATc                                                                                               >  1:163404/1‑140 (MQ=255)                                    gCTGACACGCGCATCGCCCTGGAAAGGATAGCGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATc                                                                                               >  2:638497/1‑140 (MQ=255)                                               cATCGCCCTGGAAAGGATAGCGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGc                                                                                    <  2:32155/140‑1 (MQ=255)                                                aTCGCCCTGGAAAGGATAGCGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCa                                                                                   <  1:9026/140‑1 (MQ=255)                                                          aaaGGATAGCGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGc                                                                         <  2:196903/140‑1 (MQ=255)                                                              gATAGCGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGc                                                                         >  1:796485/1‑136 (MQ=255)                                                              gATAGCGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGc                                                                         <  2:796485/136‑1 (MQ=255)                                                                   cGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAg                                                                          <  2:790781/130‑1 (MQ=255)                                                                   cGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAg                                                                          >  1:790781/1‑130 (MQ=255)                                                                   cGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACg                                                                >  2:769242/1‑140 (MQ=255)                                                                     ttGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTAcagg                                                              <  1:891601/140‑4 (MQ=255)                                                                        cagcaATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAACAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACggg                                                              >  1:576194/1‑137 (MQ=255)                                                                        cagcaATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAACAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACggg                                                              <  2:576194/137‑1 (MQ=255)                                                                           caATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCa                                                        <  1:507917/140‑1 (MQ=255)                                                                                 aTCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGa                                                  <  2:491481/140‑1 (MQ=255)                                                                                 aTCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGa                                                  <  1:187995/140‑1 (MQ=255)                                                                                     gCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGc                                              >  1:605641/1‑140 (MQ=255)                                                                                         cTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATaa                                          <  1:799572/140‑1 (MQ=255)                                                                                           tCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACg                                        <  1:769242/140‑1 (MQ=255)                                                                                           tCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACg                                        <  1:638497/140‑1 (MQ=255)                                                                                                      cTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCa                             >  1:258133/1‑140 (MQ=255)                                                                                                       tCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCAt                            >  1:559217/1‑140 (MQ=255)                                                                                                                aTCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGc                   <  2:258133/140‑1 (MQ=255)                                                                                                                 tCAGTTGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCg                  >  2:599047/1‑140 (MQ=255)                                                                                                                     ttGCCAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGTTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGa              >  2:61806/1‑140 (MQ=255)                                                                                                                         cAGGTTAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCggggggggg          >  2:51382/1‑139 (MQ=255)                                                                                                                              tAGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGa     <  1:61806/140‑1 (MQ=255)                                                                                                                               aGTTGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGGGGGGGGGGAg    >  2:563639/1‑140 (MQ=255)                                                                                                                                 ttGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCAGGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGggggggggggggggcg  >  2:838303/1‑134 (MQ=255)                                                                                                                                 ttGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGggggggggggggggga  >  2:558637/1‑134 (MQ=255)                                                                                                                                 ttGATCCGGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAAAGCTCCTCAAGCATTTCATTGGCGgggggggggggggggg  >  2:242835/1‑134 (MQ=255)                                                                                                                                        |                                                                                                                                     TCCAGCAGAGACGCCAGTCGCGCCATTAAAGTCTGGCTGACACGCGCATCGCCCTGGAAAGGATAGCGTTGCAGCAATAAATCGGCAGCTTCGCGTGACTGCTCGTTCTGCATCAGTTGCCAGGTTAGTTGATCCAGGCGGGTAAGATTTGCCGGTTCTTGCTGATACAGCAATCGTGCCAGACGCAGAGCTTCAGCCTTGTTACGGGTCGCCACGCTGACAGCATAACGCTCCTCAAGCATTTCATTGGCGGGGAGGGTGGCGAGCA  >  NC_000913/589675‑589942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑