Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	626,292	A→G	T75A (ACC→GCC)	entE →	2,3‑dihydroxybenzoate‑AMP ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	626,292	0	A	G	100.0%	169.2 / NA	47	T75A (ACC→GCC)	entE	2,3‑dihydroxybenzoate‑AMP ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/26);  total (21/26)

ATTCTGACGCGACATGCTGCGAGTGACAGCATCGCGGTTATCGACGGCGAGCGACAGTTGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAACCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGCGTAGTGAACTGAACGCCTATGCCAGCCAGAT  >  NC_000913/626157‑626425                                                                                                                                        |                                                                                                                                      aTTCTGACGCGACATGCTGCGAGTGACAGCATCGCGGTTATCGACGGCGAGCGACAGTTGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCcgc                                                                                                                                   <  1:43849/140‑1 (MQ=255)  ttCTGACGCGACATGCTGCGAGTGACAGCATCGCGGTTATCGACGGCGAGCGACAGTTGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCcgcg                                                                                                                                  <  2:638873/140‑1 (MQ=255)   tCTGACGCGACATGCTGCGAGTGACAGCATCGCGGTTATCGACGGCGAGCGACAGTTGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCgcgc                                                                                                                                 <  2:450055/140‑1 (MQ=255)      gACGCGACATGCTGCGAGTGACAGCATCGCGGTTATCGACGGCGAGCGACAGTTGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTgg                                                                                                                              <  1:548310/140‑1 (MQ=255)        cgcgACATGCTGCGAGTGACAGCATCGCGGTTATCGACGGCGAGCGACAGTTGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTa                                                                                                                            <  2:837228/140‑1 (MQ=255)        cgcgACATGCTGCGAGTGACAGCATCGCGGTTATCGACGGCGAGCGACAGTTGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTa                                                                                                                            <  2:694655/140‑1 (MQ=255)          cgACATGCTGCGAGTGACAGCATCGCGGTTATCGACGGCGAGCGACAGTTGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACa                                                                                                                          >  2:451686/1‑140 (MQ=255)              aTGCTGCGAGTGACAGCATCGCGGTTATCGACGGCGAGCGACAGTTGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCcgc                                                                                                                                   >  1:772192/1‑127 (MQ=255)              aTGCTGCGAGTGACAGCATCGCGGTTATCGACGGCGAGCGACAGTTGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCcgc                                                                                                                                   <  2:772192/127‑1 (MQ=255)                 ctgcGAGTGACAGCATCGCGGTTATCGACGGCGAGCGACAGTTGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGt                                                                                                                   >  2:555024/1‑140 (MQ=255)                   gcGAGTGACAGCATCGCGGTTATCGACGGCGAGCGACAGTTGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTaa                                                                                                                 <  1:877167/140‑1 (MQ=255)                     gAGTGACAGCATCGCGGTTATCGACGGCGAGCGACAGTTGAGTTATCGGTAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACg                                                                                                               <  2:799320/140‑1 (MQ=255)                      aGTGACAGCATCGCGGTTATCGACGGCGAGCGACAGTTGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGt                                                                                                              >  2:812533/1‑140 (MQ=255)                          aCAGCATCGCGGTTATCGACGGCGAGCGACAGTTGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCt                                                                                                          <  2:750667/140‑1 (MQ=255)                             gCATCGCGGTTATCGACGGCGAGCGACAGTTGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGaa                                                                                                       <  1:37754/140‑1 (MQ=255)                                     gTTATCGACGGCGAGCGACAGTTGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTatat                                                                                               >  1:12494/1‑140 (MQ=255)                                      ttATCGACGGCGAGCGACAGTTGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATAtt                                                                                              <  1:499039/140‑1 (MQ=255)                                      ttATCGACGGCGAGCGACAGTTGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATAtt                                                                                              >  2:524673/1‑140 (MQ=255)                                                cgagcgaCAGTTGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTcg                                                                                    >  2:429213/1‑140 (MQ=255)                                                 gagcgaCAGTTGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTcgc                                                                                   <  1:524673/140‑1 (MQ=255)                                                 gagcgaCAGTTGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTcgc                                                                                   <  1:451686/140‑1 (MQ=255)                                                  agcgaCAGTTGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTcgcg                                                                                  <  2:211861/140‑1 (MQ=255)                                                  agcgaCAGTTGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTcgcg                                                                                  >  2:385244/1‑140 (MQ=255)                                                       cAGTTGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCgctgct                                                                             <  1:812533/140‑1 (MQ=255)                                                          ttGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGaa                                                                          >  2:642999/1‑140 (MQ=255)                                                                   cGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGt                                                                 <  1:642999/140‑1 (MQ=255)                                                                    gggAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGtt                                                                >  2:98497/1‑140 (MQ=255)                                                                       aGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTgcg                                                             >  2:902957/1‑140 (MQ=255)                                                                            aaTCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGt                                                        >  2:114221/1‑140 (MQ=255)                                                                                 ggcggcGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTgg                                                   >  2:167238/1‑140 (MQ=255)                                                                                   cggcggATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCg                                                 >  2:760166/1‑140 (MQ=255)                                                                                      cggATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCgttg                                              >  1:608592/1‑140 (MQ=255)                                                                                      cggATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCgttg                                              >  1:732137/1‑140 (MQ=255)                                                                                      cggATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCgttg                                              >  2:154711/1‑140 (MQ=255)                                                                                            aCCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGc                                        >  1:112329/1‑140 (MQ=255)                                                                                                  cgTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAg                                  >  2:457005/1‑140 (MQ=255)                                                                                                   gtgtAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGc                                 >  2:23009/1‑140 (MQ=255)                                                                                                    tgtAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGCg                                <  2:732137/140‑1 (MQ=255)                                                                                                      tAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGCGTa                              <  2:156304/140‑1 (MQ=255)                                                                                                           tACGCCGTCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGCGTAGtgaa                         <  1:167238/140‑1 (MQ=255)                                                                                                                  tCAGGGCATTAAACCTGGTGAAGCCGCGCTGGTATAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCAGGCGTTGTTCAGCCATCAGCGTAGTGAACTGAACg                  >  2:664666/1‑140 (MQ=255)                                                                                                                   cAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGCGTAGTGAACTGAACGc                 <  1:98497/140‑1 (MQ=255)                                                                                                                   cAGGGCATTAAACCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGCGTAGTGAACTGAACGc                 <  2:608592/140‑1 (MQ=255)                                                                                                                             aaCCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGCGTAGTGAACTGAACGCCTATgccagc       <  1:183456/140‑1 (MQ=255)                                                                                                                             aaCCTGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGCGTAGTGAACTGAACGCCTATgccagc       <  1:154711/140‑1 (MQ=255)                                                                                                                                 tGGTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGCGTAGTGAACTGAACGCCTATGCCAGCCAGa   <  1:114221/140‑1 (MQ=255)                                                                                                                                  ggTGAAGCCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGCGTAGTGAACTGAACGCCTATGCCAGCCAGAt  <  1:760166/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      ATTCTGACGCGACATGCTGCGAGTGACAGCATCGCGGTTATCGACGGCGAGCGACAGTTGAGTTATCGGGAGCTGAATCAGGCGGCGGATAACCTCGCGTGTAGTTTACGCCGTCAGGGCATTAAACCTGGTGAAACCGCGCTGGTACAACTGGGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCATCAGCGTAGTGAACTGAACGCCTATGCCAGCCAGAT  >  NC_000913/626157‑626425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑