Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	742,127	A→G	Y144H (TAT→CAT)	dtpD ←	dipeptide:H(+) symporter DtpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	742,127	0	A	G	100.0%	189.6 / NA	53	Y144H (TAT→CAT)	dtpD	dipeptide:H(+) symporter DtpD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/22);  total (31/22)

ATTGCCACATAAGAAAATGACCAGACCCGCGATCATGCCAACCGCCGCCAGGCCAAAGCCCATCGCCCAGCTGTACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGAT  >  NC_000913/741990‑742261                                                                                                                                          |                                                                                                                                       aTTGCCACATAAGAAAATGACCAGACCCGCGATCATGCCAACCGCCGCCAGGCCAAAGCCCATCGCCCAGCTGTACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATGCa                                                                                                                                      >  1:857298/1‑140 (MQ=255) aTTGCCACATAAGAAAATGACCAGACCCGCGATCATGCCAACCGCCGCCAGGCCAAAGCCCATCGCCCAGCTGTACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATGCa                                                                                                                                      >  1:65820/1‑140 (MQ=255)            aGAAAATGACCAGACCCGCGATCATGCCAACCGCCGCCAGGCCAAAGCTCATCGCCCAGCTGTACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATGCATCAGCGAGaaa                                                                                                                           >  1:717409/1‑140 (MQ=255)                        cccccGCGATAAGCCCACCCGCCGCCAGGCCAAAGCCCACCGCCCAGCTGTCCTCTCCCGGGGCGAACCCACAGGCGATAGGTGCGATAATAGCCCCCCCGTTCCCCGCCGCATGCATCAGCGAGAAACCGCCATCacga                                                                                                               <  2:571431/138‑1 (MQ=255)                                         cccgccgCCAGGCCAAAGCCCACCGCCCAGCTGTACTTTCCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGCCCCCCCTTTCCCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTc                                                                                              <  2:920586/139‑1 (MQ=255)                                         ccccccgccgGGCAAACGCTCACCGCCCAGGTGTCCTCTCCGGGGGCGTAACCACGGGGGAGGGGTGCGATAAAAGCCCCCCCGTTGCCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTc                                                                                              <  2:717409/136‑1 (MQ=255)                                         aCCGCCGCCAGGCCAAAGCCCATCGCCCAGCTGTACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTc                                                                                              >  1:323916/1‑140 (MQ=255)                                             ccgccAGGCCAAAGCCCATCGCCCAGCTGTACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATAc                                                                                          >  2:801273/1‑140 (MQ=255)                                             ccgccAGGCCAAAGCCCATCGCCCAGCTGTACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATAc                                                                                          >  1:178271/1‑140 (MQ=255)                                                     ccAAAGCCCATCGCCCAGCTGTACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCTGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGcc                                                                                  >  1:839420/1‑140 (MQ=255)                                                        aaGCCCATCGCCCAGCTGTACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                                               >  1:140968/1‑140 (MQ=255)                                                        aaGCCCATCGCCCAGCTGTACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                                               >  1:810868/1‑140 (MQ=255)                                                            ccATCGCCCAGCTGTCCTCTTCCTGGGCGTACCCACGGGCGATAGGTGGGATAATAGCCCCCACGTTCCCCGCCGCAAGCATCAGCGAGAAACCGCTATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGa                                                                           <  2:857298/140‑1 (MQ=255)                                                                 ccccAGCGTTCCTCTCCCTGGGCGAAACCACAGGCGATGGGTGCAATAATAGCCCCCCCGTTCCCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                                      <  2:654002/139‑1 (MQ=255)                                                                           tctctTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGCCCCCACGTTCCCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                                                            <  1:45055/139‑1 (MQ=255)                                                                                tCCTGGGCGTACCCACAGGCGATGGGGGCGATAATAGCCCCCACTTTACCCGCCGCATGCATCAGCGAGAACCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                                       <  2:178271/140‑1 (MQ=255)                                                                                cccTGGGCGAACCCACGGGCGATAGGTGCGATAATAGCCCCCACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                                       <  2:323916/139‑1 (MQ=255)                                                                                cccTGGCCGTACCCACAGGCGATAGGTGGGATAAAAGCCCCCGCTTTCCCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                                       <  2:140968/139‑1 (MQ=255)                                                                                 ccTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTCCCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                                         <  1:269464/137‑1 (MQ=255)                                                                                 ccTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                                         >  2:269464/1‑137 (MQ=255)                                                                                 ccTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAAc                                                      >  1:524208/1‑140 (MQ=255)                                                                                 ccTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAAc                                                      >  1:101688/1‑140 (MQ=255)                                                                                                     aTAGGTGCGATAATAGACCCCACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGAt                                  >  2:795434/1‑140 (MQ=255)                                                                                                      tAGGTGCGATAATAGACCCCACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATa                                 >  1:539614/1‑140 (MQ=255)                                                                                                      tAGGTGCGATAATAGACCCCACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATa                                 >  1:318348/1‑140 (MQ=255)                                                                                                           gCGATAATAGACCCCACGTTACCCGCTGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc                            <  2:839420/140‑1 (MQ=255)                                                                                                           gCGATAATAGACCCCACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc                            <  1:801273/140‑1 (MQ=255)                                                                                                            gaataataGCCCCCCCTTTCCCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGcc                           <  2:318348/138‑1 (MQ=255)                                                                                                              ataataGACCCCACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAg                         >  1:383428/1‑140 (MQ=255)                                                                                                                aataGCCCCCCCTTTCCCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAggg                       <  2:524723/140‑1 (MQ=255)                                                                                                                 aaagcccccACTTTCCCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGa                      <  2:465890/135‑1 (MQ=255)                                                                                                                    gACCCCACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAg                   >  1:465890/1‑140 (MQ=255)                                                                                                                     aCCCCACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAggg                       <  1:760693/135‑1 (MQ=255)                                                                                                                     aCCCCACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAggg                       >  2:760693/1‑135 (MQ=255)                                                                                                                      ccccACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGAt                 >  1:374575/1‑140 (MQ=255)                                                                                                                       ccccCGTTCCCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATa                <  1:271574/140‑1 (MQ=255)                                                                                                                       cccACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATa                >  1:879406/1‑140 (MQ=255)                                                                                                                        ccACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATag               >  1:524723/1‑140 (MQ=255)                                                                                                                        ccACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATag               <  2:539614/140‑1 (MQ=255)                                                                                                                         cACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAAt                               <  1:576609/123‑1 (MQ=255)                                                                                                                         cACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAAt                               >  2:576609/1‑123 (MQ=255)                                                                                                                         cACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGcc                           >  1:639621/1‑127 (MQ=255)                                                                                                                         cACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGcc                           <  2:639621/127‑1 (MQ=255)                                                                                                                         cACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGAc                     <  2:163493/133‑1 (MQ=255)                                                                                                                         cACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGAc                     >  1:163493/1‑133 (MQ=255)                                                                                                                         cACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATaga              >  2:775840/1‑140 (MQ=255)                                                                                                                         cACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATaga              >  1:754196/1‑140 (MQ=255)                                                                                                                         cACGTTACCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATaga              >  1:283112/1‑140 (MQ=255)                                                                                                                               aCCCGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATg        >  2:558481/1‑140 (MQ=255)                                                                                                                                cccGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGa       >  2:68545/1‑140 (MQ=255)                                                                                                                                cccGCCGCATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGa       >  2:228111/1‑140 (MQ=255)                                                                                                                                    ccgcATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACgga   <  1:566326/140‑1 (MQ=255)                                                                                                                                     cgcATGCATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACggat  >  2:407790/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       ATTGCCACATAAGAAAATGACCAGACCCGCGATCATGCCAACCGCCGCCAGGCCAAAGCCCATCGCCCAGCTGTACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGAT  >  NC_000913/741990‑742261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑