Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 810,745 A→G K122R (AAA→AGA)  bioF → 8‑amino‑7‑oxononanoate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913810,7450AG100.0% 146.9 / NA 42K122R (AAA→AGA) bioF8‑amino‑7‑oxononanoate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/19);  total (23/19)

GTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAAAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGCGCGATTGCTTGCTTCCCCCTGTCCG  >  NC_000913/810610‑810881
                                                                                                                                       |                                                                                                                                        
gTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGagagag                                                                                                                                      <  2:380059/140‑1 (MQ=255)
   aCGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAGAGAGGAc                                                                                                                                   <  1:175298/140‑1 (MQ=255)
   aCGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAGAGAGGAc                                                                                                                                   <  2:113578/140‑1 (MQ=255)
     gTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAGAGAGGACCg                                                                                                                                 >  1:253087/1‑140 (MQ=255)
               ataGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGcc                                                                                                                       >  1:932127/1‑140 (MQ=255)
                 aGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGa                                                                                                                     <  2:253087/140‑1 (MQ=255)
                       gtgCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGa                                                                                                                     >  2:70565/1‑134 (MQ=255)
                       gtgCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGa                                                                                                                     <  1:70565/134‑1 (MQ=255)
                           aTCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGc                                                                                                           >  1:471141/1‑140 (MQ=255)
                               ggCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATg                                                                                                       >  1:42038/1‑140 (MQ=255)
                                    tGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCa                                                                                                  >  1:445961/1‑140 (MQ=255)
                                      gaagaaGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCt                                                                                                    <  2:957856/136‑1 (MQ=255)
                                      gaagaaGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCt                                                                                                    >  1:957856/1‑136 (MQ=255)
                                      gaagaaGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCAtt                                                                                                >  1:671520/1‑140 (MQ=255)
                                                 ggCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTg                                                                                     >  2:875500/1‑140 (MQ=255)
                                                  gCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGc                                                                                    <  1:254694/140‑1 (MQ=255)
                                                            ttggctATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGc                                                                          >  2:75551/1‑140 (MQ=255)
                                                            ttggctATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGc                                                                          <  2:445961/140‑1 (MQ=255)
                                                                               gctgTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGtt                                                       <  2:471141/140‑1 (MQ=255)
                                                                                        ctctGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATa                                              >  1:203667/1‑140 (MQ=255)
                                                                                         tctGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATaa                                             <  2:932127/140‑1 (MQ=255)
                                                                                             gTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTGGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGAt                                         >  2:669613/1‑140 (MQ=255)
                                                                                                              gCAGTTATTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGcgcg                        >  2:867922/1‑140 (MQ=255)
                                                                                                              gCAGTTATTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGcgcg                        >  2:748405/1‑140 (MQ=255)
                                                                                                              gCAGTTATTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGcgcg                        <  1:748405/140‑1 (MQ=255)
                                                                                                                  ttATTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGCGCGAttg                    >  2:835486/1‑140 (MQ=255)
                                                                                                                   tATTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGTCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGCGCGAttgc                   >  1:952671/1‑140 (MQ=255)
                                                                                                                   tATTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGCGCGAttgc                   <  1:669613/140‑1 (MQ=255)
                                                                                                                   tATTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGCGCGAttgc                   >  2:770765/1‑140 (MQ=255)
                                                                                                                    aTTGCCGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGCGCGAttgct                  >  1:586154/1‑140 (MQ=255)
                                                                                                                        ccGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGCGCGAttgc                   >  1:326468/1‑135 (MQ=255)
                                                                                                                        ccGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGCGCGAttgc                   <  2:326468/135‑1 (MQ=255)
                                                                                                                        ccGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGCGCGAttgc                   >  1:811118/1‑135 (MQ=255)
                                                                                                                        ccGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGCGCGAttgc                   <  2:811118/135‑1 (MQ=255)
                                                                                                                        ccGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGCGCGATtgcttgct              >  1:671529/1‑140 (MQ=255)
                                                                                                                        ccGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGCGCGATtgcttgct              >  2:652869/1‑140 (MQ=255)
                                                                                                                        ccGCGATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATATCACTCATTTGGCGCGATtgcttgct              <  1:360705/140‑1 (MQ=255)
                                                                                                                         cgcgATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGTCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGCGCGATTgcttgctt             <  2:671520/140‑1 (MQ=255)
                                                                                                                           cgATGATGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGCGCGATTGCTTGCTTcc           <  1:770765/140‑1 (MQ=255)
                                                                                                                            gatgatGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGgcg                          >  1:257148/1‑124 (MQ=255)
                                                                                                                            gatgatGGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGgcg                          <  2:257148/124‑1 (MQ=255)
                                                                                                                             atgatgGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGCGCGATTGCTTGCTTcccc         <  2:203667/140‑1 (MQ=255)
                                                                                                                             atgatgGCGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGCGCGATTGCTTGCTTcccc         <  1:685125/140‑1 (MQ=255)
                                                                                                                                    cGAGAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCCGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGCGCGATTGCTTGCTTCCCCCTGTCCg  <  2:175494/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                        
GTCACGTCAGCGGTTATAGCGTGGTGCATCAGGCACTGGAAGAAGAGCTGGCCGAGTGGCTTGGCTATTCGCGGGCACTGCTGTTTATCTCTGGTTTCGCCGCTAATCAGGCAGTTATTGCCGCGATGATGGCGAAAGAGGACCGTATTGCTGCCGACCGGCTTAGCCATGCCTCATTGCTGGAAGCTGCCAGTTTAAGCCCGTCGCAGCTTCGCCGTTTTGCTCATAACGATGTCACTCATTTGGCGCGATTGCTTGCTTCCCCCTGTCCG  >  NC_000913/810610‑810881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑