Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	114,650	C→T	A359T (GCG→ACG)	hofC ←	inner membrane protein HofC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	114,650	0	C	T	100.0%	195.0 / NA	56	A359T (GCG→ACG)	hofC	inner membrane protein HofC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/25);  total (31/25)

CCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGCCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGC  >  NC_000913/114516‑114783                                                                                                                                       |                                                                                                                                      ccAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAgcg                                                                                                                                  <  2:949129/140‑1 (MQ=255)   aGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGcc                                                                                                                                >  2:857802/1‑140 (MQ=255)               tcccaCTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTcc                                                                                                                    >  1:684698/1‑140 (MQ=255)                    cTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCgatg                                                                                                               >  1:228026/1‑140 (MQ=255)                    cTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCgatg                                                                                                               >  1:604491/1‑140 (MQ=255)                     tCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCAtt                                                                                                                             <  2:467578/125‑1 (MQ=255)                     tCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCAtt                                                                                                                             >  1:467578/1‑125 (MQ=255)                         cgcatcgcCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCgatgatgat                                                                                                          >  2:162122/1‑140 (MQ=255)                         cgcatcgcCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCgatgatgat                                                                                                          >  2:220765/1‑140 (MQ=255)                         cgcatcgcCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGATATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCgatgatgat                                                                                                          >  1:696483/1‑140 (MQ=255)                            atcgcCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATggg                                                                                                       <  2:228026/140‑1 (MQ=255)                            atcgcCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATggg                                                                                                       >  1:754832/1‑140 (MQ=255)                                  tAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGt                                                                                                 <  2:128272/140‑1 (MQ=255)                                  tAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGt                                                                                                 <  1:770727/140‑1 (MQ=255)                                        gAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTa                                                                                           <  1:220765/140‑1 (MQ=255)                                            aTTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACAt                                                                                       <  2:295054/140‑1 (MQ=255)                                                        aTTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCagaga                                                                           >  1:112405/1‑140 (MQ=255)                                                          tGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGc                                                                         >  1:394735/1‑140 (MQ=255)                                                            ccaccaccaGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCg                                                                       <  2:696483/140‑1 (MQ=255)                                                              accaccaGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCg                                                                       <  1:962121/138‑1 (MQ=255)                                                              accaccaGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCg                                                                       >  2:962121/1‑138 (MQ=255)                                                               ccaccaGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGAt                                                                    >  1:703604/1‑140 (MQ=255)                                                                 accaGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGc                                                                  <  1:400875/140‑1 (MQ=255)                                                                      cGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGa                                                                     <  1:411889/132‑1 (MQ=255)                                                                      cGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGa                                                                     >  2:411889/1‑132 (MQ=255)                                                                      cGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCtctc                                                             >  1:844457/1‑140 (MQ=255)                                                                                atTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCAc                                                   <  2:684698/140‑1 (MQ=255)                                                                                   cctccCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTaa                                                >  2:586434/1‑140 (MQ=255)                                                                                       ccGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGc                                            >  1:795249/1‑140 (MQ=255)                                                                                        cGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCAc                                                   >  1:148419/1‑132 (MQ=255)                                                                                        cGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCAc                                                   <  2:148419/132‑1 (MQ=255)                                                                                         gTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCaa                                          <  2:112405/140‑1 (MQ=255)                                                                                           tATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAAc                                        >  1:355151/1‑140 (MQ=255)                                                                                               aTCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACagag                                    <  1:168006/140‑1 (MQ=255)                                                                                               aTCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACagag                                    <  2:703604/140‑1 (MQ=255)                                                                                                tCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGc                                   <  2:43311/140‑1 (MQ=255)                                                                                                tCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGc                                   <  2:394735/140‑1 (MQ=255)                                                                                                  aGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCgg                                 >  1:99670/1‑140 (MQ=255)                                                                                                  aGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCgg                                 <  2:443209/140‑1 (MQ=255)                                                                                                  aGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCgg                                 >  2:923609/1‑140 (MQ=255)                                                                                                  aGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCgg                                 >  1:419782/1‑140 (MQ=255)                                                                                                    cAACGCCGGTTCCAGTAAGGCTGCGAGGATATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGc                               >  2:699680/1‑140 (MQ=255)                                                                                                       cGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTaa                            >  1:536360/1‑140 (MQ=255)                                                                                                        gCCGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTaaa                           <  2:910083/140‑1 (MQ=255)                                                                                                         ccGGTTCCAGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAAc                          >  1:890757/1‑140 (MQ=255)                                                                                                                 aGTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGt                  <  1:461880/140‑1 (MQ=255)                                                                                                                  gTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTa                 >  2:698833/1‑140 (MQ=255)                                                                                                                  gTAAGGCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTa                 >  2:910983/1‑140 (MQ=255)                                                                                                                      ggCTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTAtttt             >  2:522988/1‑140 (MQ=255)                                                                                                                        cTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTaaa                           >  2:846443/1‑124 (MQ=255)                                                                                                                        cTGCGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTaaa                           <  1:846443/124‑1 (MQ=255)                                                                                                                           cGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAgc        >  1:54002/1‑140 (MQ=255)                                                                                                                           cGAGGTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAgc        >  2:939255/1‑140 (MQ=255)                                                                                                                               gTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCTGTATTTTTTAGCgcca    <  1:699680/140‑1 (MQ=255)                                                                                                                               gTTATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCgcca    <  1:923609/140‑1 (MQ=255)                                                                                                                                 tATCCGTCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCgccagc  <  2:604491/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      CCAGCGTTATCCCATCCCACTCATCGCATCGCCTAAATGGAAAATTGGCAGATACATTGCCACCACCAGCGTACCAATAATTCCTCCCGTTATGATCAGCAACGCCGGTTCCAGTAAGGCTGCGAGGTTATCCGCCAGCGCCATTGTGTTTTCCCGATGATGATGGGCGAGGTTGTCTAACATGAGATCCAGAGAGCCGGATGCCTCTCCTGTTCTCACTAATTGCAAACAGAGCGGGCTAAACTCACCGGTATTTTTTAGCGCCAGC  >  NC_000913/114516‑114783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑