Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	892,129	T→C	L55L (TTA→CTA)	rimK →	ribosomal protein S6 modification protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	892,129	0	T	C	100.0%	106.6 / NA	31	L55L (TTA→CTA)	rimK	ribosomal protein S6 modification protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/10);  total (21/10)

TATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGAC  >  NC_000913/892003‑892263                                                                                                                               |                                                                                                                                       tATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGCTACCCCATTTTGa                                                                                                                           <  2:497541/140‑1 (MQ=255)      gtgtAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGCTACCCCATTTTGACGCAg                                                                                                                      <  2:759742/140‑1 (MQ=255)       tgtAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGCTACCCCATTTTGa                                                                                                                           >  2:581708/1‑134 (MQ=255)       tgtAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGCTACCCCATTTTGa                                                                                                                           <  1:581708/134‑1 (MQ=255)         tAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGCTAccc                                                                                                                                   <  2:383025/124‑1 (MQ=255)         tAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGCTAccc                                                                                                                                   >  1:383025/1‑124 (MQ=255)             cGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGCTACCCCATTTTGACGCAGTGATccc                                                                                                               >  1:579895/1‑140 (MQ=255)              ggCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGCTACCCCATTTTGACGCAGTGATCCcg                                                                                                              <  1:486940/140‑1 (MQ=255)                         gCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGCTCCCCCATTTTGACGCAGTGATCCCGCGTATTGGCAc                                                                                                   <  2:667033/140‑1 (MQ=255)                          ccGCTATACAGCGCGGTCACCTGGATGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGCTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCAcc                                                                                                  >  2:697713/1‑140 (MQ=255)                             cTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGCTACCCCATTTTGACGCAGTGATCCcg                                                                                                              <  1:153490/125‑1 (MQ=255)                             cTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGCTACCCCATTTTGACGCAGTGATCCcg                                                                                                              >  2:153490/1‑125 (MQ=255)                                taCAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGATTTTTCTATTCACTACAAAGGCCGCAAGCTCCCCCATTTTGACGCAGTGACCCCGCGTATTGGCACCGCCAtt                                                                                            <  2:935689/140‑1 (MQ=255)                                  cAGCGCGGTCCCCTGGTTAAATTTTTTGTTCCGTTTTCTTGCTACATAAACATAAATCCTGCGGCGTCTTCTATTCTCTACAAAGCCCGCAACCTCCCCCATTTTGACGCAGTGCTCCCGCTTATTGGCACCGCCATTAc                                                                                          <  2:317546/140‑1 (MQ=255)                                     cgcgGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGCTACCCGATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCtt                                                                                       >  1:318851/1‑140 (MQ=255)                                     cgcgGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGCTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCtt                                                                                       >  2:601656/1‑140 (MQ=255)                                          tCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGCTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCTTTTATg                                                                                  >  1:52938/1‑140 (MQ=255)                                          tCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGCTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCTTTTATg                                                                                  >  2:619105/1‑140 (MQ=255)                                                 gTTGAATTTCTTGCCCCGCTTTCTTGCTACATAAACATAAATCCTGCGGCGTCTTCTATTCACTACAAGGGCCGCAAGCTCCCCGTTTTTGACGCAGTGACCCCGCGTATTGGCACCGCCATTACCTTTTATGGGAcggc                                                                           <  2:318851/140‑1 (MQ=255)                                                                  gCTTTCTTGCTACATGAACATAAATCCTGCGGTGTCTTCTATTCACTAAAAAGGCCGCAAGCTCCCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCTTTTATGGAACGGCGGCACTGCGCCAGTTCg                                                          <  1:259151/140‑1 (MQ=255)                                                                     ttCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGCTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCgaga                                                       <  1:619105/140‑1 (MQ=255)                                                                     ttCTTACTACATAAAAATAAACCCTGCGGCTTCTTCTATTCACAAAAACGGCCGCAGGCTCCCCCTTTTTGCCGCAGGGCCCCCGCGTATTGGCACCGCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCgaga                                                       <  2:77408/140‑1 (MQ=255)                                                                              acaaaaCAAAAATCCTGCGGCGTCTTATATTCACTACAAAGGCCGCAACCTCCCCCATTTTGACGGGGTGCCCCCGAGTATGGGCACCGCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGa                                              <  2:160924/136‑1 (MQ=255)                                                                                      tAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGCTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCAATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCg                                      <  2:579895/140‑1 (MQ=255)                                                                                        aaTCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGCTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCt                                    >  1:954354/1‑140 (MQ=255)                                                                                             tGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGCTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCTcctgt                               >  1:160924/1‑136 (MQ=255)                                                                                               aaacagcttcttTTCACTACAAGGGCCGCAAGCTCCCCCTTTTTGACGCGGTGATCCCGCTTATTGGCACCGCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCTCAATGAg                             <  2:155780/134‑1 (MQ=255)                                                                                                    tcttctTTTCACAAAAAGGCCCGCAAGCTCCCCCATTTTGACGCAGTGACCCCGCGTATGGGCACCGCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCTCAATGAgtcggt                        <  2:954354/140‑1 (MQ=255)                                                                                                    tcttctATTCACTACAAAGGCCGCAAGCTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCTCAATGAgtcggt                        >  2:448010/1‑140 (MQ=255)                                                                                                               cTACAAAGGCCGCAAGCTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGccc             >  2:155477/1‑140 (MQ=255)                                                                                                                tACAAAGGCCGCAAGCTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCAtctgtct            >  1:155780/1‑134 (MQ=255)                                                                                                                tACAAAGGCCGCAAGCTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCg            >  2:108328/1‑140 (MQ=255)                                                                                                                tACAAAGGCCGCAAGCTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCg            >  1:724617/1‑140 (MQ=255)                                                                                                                  cAAAGGCCGCAAGCTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCggg          >  1:37678/1‑140 (MQ=255)                                                                                                                   aaaGGCCGCAAGCTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGgc         <  1:448010/140‑1 (MQ=255)                                                                                                                     aGGCCGCAAGCTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGccctgtctc       >  1:960206/1‑134 (MQ=255)                                                                                                                       gCCGCAAGCTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGc                     <  2:260918/124‑1 (MQ=255)                                                                                                                       gCCGCAAGCTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGc                     >  1:260918/1‑124 (MQ=255)                                                                                                                       gCCGCAAGCTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGt     >  1:502270/1‑140 (MQ=255)                                                                                                                       gCCGCAAGCTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCTTTTATGGGACGGCGACACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGt     >  2:802675/1‑140 (MQ=255)                                                                                                                          gCAAGCTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGAc  <  2:52938/140‑1 (MQ=255)                                                                                                                          gCAAGCTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGAc  >  2:582495/1‑140 (MQ=255)                                                                                                                               |                                                                                                                                       TATTCGTGTAAGCGGCTGCGTGAAGCCGCTATACAGCGCGGTCACCTGGTTGAAATTCTTGATCCGCTTTCTTGCTACATGAACATAAATCCTGCGGCGTCTTCTATTCACTACAAAGGCCGCAAGTTACCCCATTTTGACGCAGTGATCCCGCGTATTGGCACCGCCATTACCTTTTATGGGACGGCGGCACTGCGCCAGTTCGAGATGCTGGGGAGCTATCCGCTCAATGAGTCGGTCGCCATTGCCCGGGCGCGTGAC  >  NC_000913/892003‑892263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑