Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	911,763	G→A	R130C (CGT→TGT)	hcr ←	NADH oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	911,763	0	G	A	100.0%	207.6 / NA	59	R130C (CGT→TGT)	hcr	NADH oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/24);  total (35/24)

CGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGAAAGCCAGAGATAATCACCGCGTTTTACATCGCGCGTCAGC  >  NC_000913/911628‑911899                                                                                                                                        |                                                                                                                                         cGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATc                                                                                                                                      >  1:336572/1‑140 (MQ=255)         ttATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAAt                                                                                                                              <  1:335444/140‑1 (MQ=255)                 tGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGa                                                                                                                      <  2:336572/140‑1 (MQ=255)                  gCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAAt                                                                                                                              <  1:345343/131‑1 (MQ=255)                  gCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAAt                                                                                                                              >  2:345343/1‑131 (MQ=255)                  gCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGAc                                                                                                                     >  1:805628/1‑140 (MQ=255)                    caccaGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGAcgc                                                                                                                   >  2:101610/1‑140 (MQ=255)                      ccaGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGAcgccg                                                                                                                 <  1:218347/140‑1 (MQ=255)                               ccGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTgcc                                                                                                        <  1:631530/140‑1 (MQ=255)                                       ttACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCaa                                                                                                >  2:841304/1‑140 (MQ=255)                                       ttACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCaa                                                                                                <  1:678143/140‑1 (MQ=255)                                       ttACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCaa                                                                                                >  2:661584/1‑140 (MQ=255)                                            ccACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGa                                                                                           >  2:584456/1‑140 (MQ=255)                                               cTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAAtt                                                                                        >  1:849885/1‑140 (MQ=255)                                                tCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAAttt                                                                                       >  1:850628/1‑140 (MQ=255)                                                tCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCCGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAAttt                                                                                       <  2:849885/140‑1 (MQ=255)                                                 cATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTa                                                                                      >  1:305674/1‑140 (MQ=255)                                                     ggCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTAtctt                                                                                  <  1:661584/140‑1 (MQ=255)                                                     ggCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTAtctt                                                                                  <  1:841304/140‑1 (MQ=255)                                                         aaaaTAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCtgctgc                                                                              >  2:884683/1‑140 (MQ=255)                                                                     tGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCa                                                                  >  2:292767/1‑140 (MQ=255)                                                                       cggcgTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAgg                                                                <  2:805628/140‑1 (MQ=255)                                                                       cggcgTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAgg                                                                >  1:780082/1‑140 (MQ=255)                                                                           gTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTaaa                                                            <  2:305674/140‑1 (MQ=255)                                                                           gTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTaaa                                                            <  1:292767/140‑1 (MQ=255)                                                                           gTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTaaa                                                            <  1:884683/140‑1 (MQ=255)                                                                             aCGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAAtt                                                          >  1:944912/1‑140 (MQ=255)                                                                                    ttGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCAt                                                   >  2:392891/1‑140 (MQ=255)                                                                                             aCCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGa                                          >  2:535885/1‑140 (MQ=255)                                                                                               ccacacaTCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGaaa                                        >  1:218836/4‑140 (MQ=255)                                                                                               ccGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGaaa                                        >  2:87540/1‑140 (MQ=255)                                                                                               ccGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGaaa                                        >  2:748461/1‑140 (MQ=255)                                                                                               ccGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGaaa                                        >  2:537964/1‑140 (MQ=255)                                                                                               ccGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGaaa                                        >  2:478030/1‑140 (MQ=255)                                                                                               ccGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGaaa                                        >  1:736145/1‑140 (MQ=255)                                                                                                 gCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGAAAGc                                      <  1:478030/140‑1 (MQ=255)                                                                                                 gCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGAAAGc                                      <  1:537964/140‑1 (MQ=255)                                                                                                      tCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGAAAGCCagag                                 <  1:748461/140‑1 (MQ=255)                                                                                                      tCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGAAAGCCagag                                 >  1:740777/1‑140 (MQ=255)                                                                                                      tCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGAAAGCCa                                    >  2:756963/1‑137 (MQ=255)                                                                                                      tCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGAAAGCCa                                    <  1:756963/137‑1 (MQ=255)                                                                                                       cGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGAAAGCCAgaga                                >  2:190847/1‑140 (MQ=255)                                                                                                         gCCTGTGGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGAAAGCCAGAGATa                              >  2:351966/1‑140 (MQ=255)                                                                                                            tgtgGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCGAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCg                                           >  2:845613/1‑124 (MQ=255)                                                                                                            tgtgGACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCg                                           <  1:845613/124‑1 (MQ=255)                                                                                                               ggACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGAAAGCCAGAGATAATCAcc                        <  1:190847/140‑1 (MQ=255)                                                                                                               ggACGGTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGAAAGCCAGAGATAATCAcc                        >  2:868498/1‑140 (MQ=255)                                                                                                                   ggTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGAAAGCCAGAGATAATCAc                         <  2:186052/135‑1 (MQ=255)                                                                                                                   ggTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGAAAGCCAGAGATAATCAc                         >  1:186052/1‑135 (MQ=255)                                                                                                                    gTTCTTCGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGAAAGCCAGAGATAATCACCGCGtt                   >  2:192597/1‑140 (MQ=255)                                                                                                                     ttcttcGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGAAAGCCAGAGATAATCAcc                        >  2:325703/1‑134 (MQ=255)                                                                                                                     ttcttcGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGAAAGCCAGAGATAATCAcc                        <  1:325703/134‑1 (MQ=255)                                                                                                                        ttcGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGAAAGCCAGAGATAATCACCGCGTTTTAc               >  2:282458/1‑140 (MQ=255)                                                                                                                        ttcGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCGTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGAAAGCCAGAGATAATCACCGCGTTTTAc               >  2:669557/1‑140 (MQ=255)                                                                                                                          cGCAAGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGAAAGCCAGAGATAATCACCGCGTTTTACAt             >  1:885577/1‑140 (MQ=255)                                                                                                                              aGCCAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGAAAGCCAGAGATAATCACCGCGTTTTACATcgcg         <  1:392891/140‑1 (MQ=255)                                                                                                                                 cAGCGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGAAAGCCAGAGATAATCACCGCGTTTTACATCGCGCGt      <  2:736145/140‑1 (MQ=255)                                                                                                                                    cGACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGAAAGCCAGAGATAATCACCGCGTTTTACATCGCGCGTCAg   >  2:543402/1‑140 (MQ=255)                                                                                                                                     gACACATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGAAAGCCAGAGATAATCACCGCGTTTTACATCGCGCGTCAGc  <  1:282458/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         CGGTAACGTTATTTTCTGCCACCAGCGTTACCGGATAGTTACGCCACTCATCGGCGAAAATAACATCCTGCGGCGTACGCACGTTGTAGATCACCCGCACATCGGCCTGTGGACGGTTCTTCGCAAGCCAGCGACGCATCGACATAATCGGCGTGACGCCGCAGCCTGCCGCCAGCAACAGGAATTTATCTTCTGCTTTATCGTCGCAGGTAAATTCCCCCATCGCGTCCGAAAGCCAGAGATAATCACCGCGTTTTACATCGCGCGTCAGC  >  NC_000913/911628‑911899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑