Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	947,956	C→T	A243V (GCG→GTG)	ycaM →	putative transporter YcaM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	947,956	0	C	T	100.0%	156.2 / NA	47	A243V (GCG→GTG)	ycaM	putative transporter YcaM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/24);  total (23/24)

CCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGCGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACA  >  NC_000913/947820‑948090                                                                                                                                         |                                                                                                                                       ccTCATATCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTggtggt                                                                                                                                     <  2:449875/140‑1 (MQ=255)     atatCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATg                                                                                                                                 >  2:145820/1‑140 (MQ=255)               tACCTACATTACCACTATTTCAATGCTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGttt                                                                                                                       <  1:532018/140‑1 (MQ=255)                 ccTACATTACCACTATTTCAATGCTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTtgt                                                                                                                     >  2:246151/1‑140 (MQ=255)                      aTTACCACTATTTCAATGCTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCAt                                                                                                                >  2:828079/1‑140 (MQ=255)                          ccACTATTTCAATGCTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTg                                                                                                            >  2:746468/1‑140 (MQ=255)                          ccACTATTTCAATGCTGGTTTTCGCGGTTGGCGGAGCAGAGAAGAATTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTAAGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTg                                                                                                            >  2:667932/1‑140 (MQ=255)                           cACTATTTCAATGCTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTgg                                                                                                           >  2:229788/1‑140 (MQ=255)                             cTATTTCAATGCTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTtgtg                                                                                                                    >  1:596321/1‑129 (MQ=255)                             cTATTTCAATGCTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTtgtg                                                                                                                    <  2:596321/129‑1 (MQ=255)                                tttCAATGCTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCg                                                                                                      >  1:399459/1‑140 (MQ=255)                                      tGCTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCg                                                                                                <  1:746468/140‑1 (MQ=255)                                       gCTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGa                                                                                               <  1:97863/140‑1 (MQ=255)                                        cTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGc                                                                                                     >  2:656219/1‑133 (MQ=255)                                        cTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGc                                                                                                     <  1:656219/133‑1 (MQ=255)                                        cTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGAt                                                                                              >  1:73624/1‑140 (MQ=255)                                        cTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGAt                                                                                              >  2:606509/1‑140 (MQ=255)                                        cTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGAt                                                                                              <  2:875368/140‑1 (MQ=255)                                          ggTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATgg                                                                                            >  1:361021/1‑140 (MQ=255)                                              ttCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGgat                                                                                        <  1:667932/140‑1 (MQ=255)                                                  cgGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGatgatg                                                                                    <  1:145820/140‑1 (MQ=255)                                                      tGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTg                                                                                >  2:895915/1‑140 (MQ=255)                                                                  gaagaTTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAata                                                                    >  1:859045/1‑140 (MQ=255)                                                                  gaagaTTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAata                                                                    >  1:33178/1‑140 (MQ=255)                                                                   aagaTTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGACTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAatat                                                                   >  2:519453/1‑140 (MQ=255)                                                                        ttCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCgg                                                              <  1:229788/140‑1 (MQ=255)                                                                           tcCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATg                                                           >  1:65463/1‑140 (MQ=255)                                                                               tACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACtt                                                       >  2:6467/1‑140 (MQ=255)                                                                                aCGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTa                                                      >  2:821374/1‑140 (MQ=255)                                                                                      aTCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGAcc                                                >  1:323190/1‑140 (MQ=255)                                                                                       tCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCa                                               <  1:821374/140‑1 (MQ=255)                                                                                       tCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCa                                               <  1:606509/140‑1 (MQ=255)                                                                                        cAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCaa                                              <  1:246151/140‑1 (MQ=255)                                                                                          aaCGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACg                                            <  2:65463/140‑1 (MQ=255)                                                                                            cgcgCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGt                                          <  2:399459/140‑1 (MQ=255)                                                                                             gcgcAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTc                                         <  1:895915/140‑1 (MQ=255)                                                                                             gcgcAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTc                                         <  1:828079/140‑1 (MQ=255)                                                                                                         aaaaGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGt                                          >  2:712474/1‑127 (MQ=255)                                                                                                         aaaaGAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGt                                          <  1:712474/127‑1 (MQ=255)                                                                                                             gAATTTCCAAAAGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCa                         >  1:279685/1‑140 (MQ=255)                                                                                                                        aGGGATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCg              <  2:279685/140‑1 (MQ=255)                                                                                                                         gggATGTTATGCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGa             <  1:6467/140‑1 (MQ=255)                                                                                                                                   gCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTacaac   <  2:33178/140‑1 (MQ=255)                                                                                                                                   gCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCAGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTacaac   <  2:859045/140‑1 (MQ=255)                                                                                                                                   gCCTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTAATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTacaac   <  2:759079/140‑1 (MQ=255)                                                                                                                                    ccTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTacaaca  <  2:536569/140‑1 (MQ=255)                                                                                                                                     cTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGa             <  2:618981/128‑1 (MQ=255)                                                                                                                                     cTGGTGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGa             >  1:618981/1‑128 (MQ=255)                                                                                                                                         |                                                                                                                                       CCTCATATCGACTTTACCTACATTACCACTATTTCAATGCTGGTTTTCGCGGTTGGCGGAGCAGAGAAGATTTCTCCTTACGTTAATCAAACGCGCAACCCAGGAAAAGAATTTCCAAAAGGGATGTTATGCCTGGCGGTGATGGTTGCGGTTTGTGCCATTCTGGGCTCGCTGGCGATGGGGATGATGTTTGATTCGCGTAATATCCCGGATGACTTAATGACCAACGGTCAGTATTACGCCTTTCAGAAGCTGGGCGAGTATTACAACA  >  NC_000913/947820‑948090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑