Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	994,316	T→A	I173F (ATC→TTC)	ssuC ←	aliphatic sulfonate ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	994,316	0	T	A	100.0%	166.2 / NA	48	I173F (ATC→TTC)	ssuC	aliphatic sulfonate ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (21/27);  total (21/27)

GCATTCATCGCCAGATAACCAATGCCTGAATTGGCAGAAATGGTTTCGGCAACAATCAGCGTCAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGATCACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGAT  >  NC_000913/994189‑994451                                                                                                                                |                                                                                                                                        gcATTCATCGCCAGATAACCAATGCCTGAATTGGCAGAAATGGTTTCGGCAACAATCAGCGTCAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATaaa                                                                                                                             <  2:785836/140‑1 (MQ=255)     tCATCGCCAGATAACCAATGCCTGAATTGGCAGAAATGGTTTCGGCAACAATCAGCGTCAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAAcaca                                                                                                                                     <  2:255182/128‑1 (MQ=255)     tCATCGCCAGATAACCAATGCCTGAATTGGCAGAAATGGTTTCGGCAACAATCAGCGTCAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAAcaca                                                                                                                                     >  1:255182/1‑128 (MQ=255)       aTCGCCAGATAACCAATGCCTGAATTGGCAGAAATGGTTTCGGCAACAATCAGCGTCAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCg                                                                                                                        <  1:572736/139‑1 (MQ=255)       aTCGCCAGATAACCAATGCCTGAATTGGCAGAAATGGTTTCGGCAACAATCAGCGTCAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCg                                                                                                                        >  2:572736/1‑139 (MQ=255)           ccAGATAACCAATGCCTGAATTGGCAGAAATGGTTTCGGCAACAATCAGCGTCAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATg                                                                                                                   <  1:73641/140‑1 (MQ=255)               aTAACCAATGCCTGAATTGGCAGAAATGGTTTCGGCAACAATCAGCGTCAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATg                                                                                                                   <  2:396319/136‑1 (MQ=255)               aTAACCAATGCCTGAATTGGCAGAAATGGTTTCGGCAACAATCAGCGTCAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATg                                                                                                                   >  1:396319/1‑136 (MQ=255)               aTAACCAATGCCTGAATTGGCAGAAATGGTTTCGGCAACAATCAGCGTCAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCgg                                                                                                               >  2:212485/1‑140 (MQ=255)               aTAACCAATGCCTGAATTGGCAGAAATGGTTTCGGCAACAATCAGCGTCAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCgg                                                                                                               >  1:956810/1‑140 (MQ=255)               aTAACCAATGCCTGAATTGGCAGAAATGGTTTCGGCAACAATCAGCGTCAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCgg                                                                                                               >  2:334011/1‑140 (MQ=255)                tAACCAATGCCTGAATTGGCAGAAATGGTTTCGGCAACAATCAGCGTCAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGa                                                                                                              <  1:723304/140‑1 (MQ=255)                tAACCAATGCCTGAATTGGCAGAAATGGTTTCGGCAACAATCAGCGTCAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGa                                                                                                              <  1:359504/140‑1 (MQ=255)                       tGCCTGAATTGGCAGAAATGGTTTCGGCAACAATCAGCGTCAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCa                                                                                                       >  1:414167/1‑140 (MQ=255)                        gCCTGAATTGGCAGAAATGGTTTCGGCAACAATCAGCGTCAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCAt                                                                                                      >  2:454289/1‑140 (MQ=255)                                 ggCAGAAATGGTTTCGGCAACAATCAGCGTCAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTAcgcg                                                                                             <  2:956810/140‑1 (MQ=255)                                  gCAGAAATGGTTTCGGCAACAATCAGCGTCAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACgcgc                                                                                            <  1:742484/140‑1 (MQ=255)                                   cAGAAATGGTTTCGGCAACAATCAGCGTCAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGcc                                                                                           <  1:337452/140‑1 (MQ=255)                                     aacaTGGTTCCGGGAACAATCAGCGTCAGCCACCTCAGGCCCAACGCAAAACGCTCGCCGATCATAATTGAGCTCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCAt                                                                                         <  2:636459/137‑1 (MQ=255)                                                    acaaTCAGCGTCAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCcgcg                                                                          <  2:348084/140‑1 (MQ=255)                                                     caaTCAGCGTCAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGcga                                                                         <  1:334011/140‑1 (MQ=255)                                                      aaTCAGCGTCAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCCCCAGCCCGcgat                                                                        <  2:850282/140‑1 (MQ=255)                                                      aaTCAGCGTCAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGcgat                                                                        <  2:557698/140‑1 (MQ=255)                                                      aaTCAGCGTCAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGcgat                                                                        <  1:212485/140‑1 (MQ=255)                                                      aaTCAGCGACAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGcgat                                                                        <  2:361367/140‑1 (MQ=255)                                                                aGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACg                                                              >  2:607962/1‑140 (MQ=255)                                                                aGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACg                                                              <  2:414167/140‑1 (MQ=255)                                                                aGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACg                                                              <  1:474108/140‑1 (MQ=255)                                                                          ggCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACGGatgccatgc                                                    >  1:936596/1‑140 (MQ=255)                                                                           gCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACGGatgccatgcc                                                   <  2:171352/140‑1 (MQ=255)                                                                           gCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACGGatgccatgcc                                                   <  2:920143/140‑1 (MQ=255)                                                                            cccAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACGGAtgccatgcca                                                  >  1:730708/1‑140 (MQ=255)                                                                               aaCGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACGGATGCCATGCCAGgt                                               <  2:532127/140‑1 (MQ=255)                                                                               aaCGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACGGATGCCATGCCAGgt                                               >  1:380933/1‑140 (MQ=255)                                                                                acacAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACGGATGCCATGCCAGgtg                                              >  2:242814/4‑140 (MQ=255)                                                                                aCGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACGGatgc                                                         >  1:725250/1‑129 (MQ=255)                                                                                aCGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACGGatgc                                                         <  2:725250/129‑1 (MQ=255)                                                                                aCGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACGGATGCCATGCCAGgtg                                              <  1:454289/140‑1 (MQ=255)                                                                                          cacgcCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACGGATGCCATGCCAGGTGTTGat                                         <  1:639838/135‑1 (MQ=255)                                                                                          cacgcCGACCATAATTGAGGGCAGGGCACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACGGATGCCATGCCAGGTGTTGat                                         >  2:639838/1‑135 (MQ=255)                                                                                                           agggcagggcACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCg                        >  1:866382/1‑135 (MQ=255)                                                                                                           agggcagggcACCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCg                        <  2:866382/135‑1 (MQ=255)                                                                                                             ggcagggcaCCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACGGATGCCATGCCAGGTGTTGATATAATTGGGGAATAGCGTACCGAg                 <  2:627792/140‑1 (MQ=255)                                                                                                             ggcagggcaCCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACGGATGCCATGCCAGGTGTTGATATAAATggg                                <  2:248854/125‑1 (MQ=255)                                                                                                             ggcagggcaCCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACGGATGCCATGCCAGGTGTTGATATAAATggg                                >  1:248854/1‑125 (MQ=255)                                                                                                             ggcagggcaCCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAg                 >  2:489716/1‑140 (MQ=255)                                                                                                                agggcaCCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAgcgc              <  2:730708/140‑1 (MQ=255)                                                                                                                 gggcaCCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGcc             >  2:723688/1‑140 (MQ=255)                                                                                                                  ggcaCCAGGCAGGAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGcca            >  1:175036/1‑140 (MQ=255)                                                                                                                            aggAACACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGAt  >  2:838448/1‑140 (MQ=255)                                                                                                                                |                                                                                                                                        GCATTCATCGCCAGATAACCAATGCCTGAATTGGCAGAAATGGTTTCGGCAACAATCAGCGTCAGCCACATCAGGCCCAACGCAAAACGCACGCCGACCATAATTGAGGGCAGGGCACCAGGCAGGATCACATGGATAAACAGCGGTATGCCGGATAATCCATAGCTACGCGCCATCTCCACCAACCCGCGATCGATATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGAT  >  NC_000913/994189‑994451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑